Spats2 (BC023432) Mouse Tagged ORF Clone

CAT#: MG207631

Spats2 (tGFP-tagged) - Mouse spermatogenesis associated, serine-rich 2 (cDNA clone MGC:32347 IMAGE:5029821)

Product Images

Circular map for MG207631

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Spats2
• Synonyms: Scr59, p59, 59kDa
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207631 representing BC023432
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGTAGTGCCAGTGAAGTGCTCAAAGAATGGATAGTAACTGGCAAAAAAAAGAACAAAAAGAAGA
  AGAGCAAGCCAAAGCCTGCATCAGAAGCAAGTGGCAGTGCCCCAGACTCCAGTAAGTCGGCCCCCATTCA
  AGAGGAGCAGCCTGCATCTTCAGAGAAAGGCAGCATCAATGGTTACCACGTAAACGGTGCCATCAACGAT
  GCCGAGTCTGTGGACTCGCTCAGTGAAGGGCTGGAGACGCTGTCAATAGATGCCAGAGAGCTGGAGGATC
  CCGAGTTTGCCGCAGCAGAGACGCTGGACAGAACTGGATCTGTGCTGGAGAATGGTGTCTCTGACTTTGA
  GCCCAAATCTTTGACTGCGCACTCTATCTCGAATGTCCAGCAATCCCGGAACGCTGCCAAATCTCTGTCC
  AGAACCACCCCAGGAGCACAGGTTTCAAATCTGGGAATGGAGAACGTTCCACTCTCTTCCACCAATAAAA
  AGCTAGGTTCTAATATTGAGAAATCGGTGAAAGACCTCCAGCGCTGCACTGTTTCTCTTGCACGGTACCG
  AGTTGTCGTTAAAGAAGAGATGGATGCGTCCATTAAGAAAATGAAACAAGCGTTTGCCGAATTGCAAAGC
  TGTTTAATGGATCGAGAAGTGGCACTGCTTGCTGAGATGGATAAAGTGAAAGCTGAAGCAATGGAAATCT
  TGCTCAGCCGGCAGAAGAAAGCGGAGCTTCTAAAGAAGATGACAGATGTGGCCGTTCGCATGTCAGAGGA
  GCAGTTGGTTGAACTCAGGGCTGATATCAAGCACTTTGTCAGTGAGCGGAAATATGATGAAGACCTTGGA
  AGGGTAGCCCGCTTCACCTGTGATGTGGAGACTCTGAAACAGAGTATCGATTCTTTCGGACAAGTGTCAC
  ATCCAAAGAACAGCTACTCAACCCGCTCTCGATGCAGCTTGGTGGCACCTGTGTCCTTGAGTGGCCCAAG
  TGATGGCTCTGCTGCTTCTTCCTCCCCTGATGCCTCTGTTCCCAGCCTCCCAGGTGCTAACAAGAGAAAC
  TGTGCCCCAAGAGAGGCCTCTGCAGCCATGACGAACTCCAGTGACCGGCCCTGCCAGGCTCATCGGGAGG
  TATTTCCAGGCAACAGGAGAGGAGGACAAGGCTACCGGGCACAAAGCCAAAAGACCGCAGACCCCTCAAA
  CCCAGGGCGCCATGACAGTGTGGGCCGCTACAGAAACAGCTCATGGTATTCTTCCGGGCCCAGGTATCAG
  GGTGTTCCGCCACAAGCCCCAGGAAACGCCGGCGAGCGGAGTCGCCCTTACTCTGCAGGGACCAATGGAA
  CTGGAGCCATCTCAGAGCCTAGCCCACCCAAGCCTTCGTTCAAAAAGGGGCTCCCACAGCGCAAACCAAG
  GGCCTCTCAGGCTGAAGCTGCGAACTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207631 representing BC023432
  Red=Cloning site Green=Tags(s)
  
  MEGSASEVLKEWIVTGKKKNKKKKSKPKPASEASGSAPDSSKSAPIQEEQPASSEKGSINGYHVNGAIND
  AESVDSLSEGLETLSIDARELEDPEFAAAETLDRTGSVLENGVSDFEPKSLTAHSISNVQQSRNAAKSLS
  RTTPGAQVSNLGMENVPLSSTNKKLGSNIEKSVKDLQRCTVSLARYRVVVKEEMDASIKKMKQAFAELQS
  CLMDREVALLAEMDKVKAEAMEILLSRQKKAELLKKMTDVAVRMSEEQLVELRADIKHFVSERKYDEDLG
  RVARFTCDVETLKQSIDSFGQVSHPKNSYSTRSRCSLVAPVSLSGPSDGSAASSSPDASVPSLPGANKRN
  CAPREASAAMTNSSDRPCQAHREVFPGNRRGGQGYRAQSQKTADPSNPGRHDSVGRYRNSSWYSSGPRYQ
  GVPPQAPGNAGERSRPYSAGTNGTGAISEPSPPKPSFKKGLPQRKPRASQAEAANS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC023432
• ORF Size: 1430 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023432, AAH23432
• RefSeq Size: 2462 bp
• RefSeq ORF: 1430 bp
• Locus ID: 72572
• Cytogenetics: 15 F1