Mboat7 (NM_029934) Mouse Tagged ORF Clone

CAT#: MG207548

Mboat7 (tGFP-tagged) - Mouse leukocyte receptor cluster (LRC) member 4 (Leng4)

Product Images

Circular map for MG207548

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mboat7
• Synonyms: 5730589L02Rik; BB1; Leng4; Lpiat; Lpiat1; mBB1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207548 representing NM_029934
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACACCCGAAGAATGGACATATCTAATGGTCCTTCTTATCTCCATCCCTGTTGGCTTCCTCTTTAAGA
  AAGCTGGACCTGGGCTGAAGAGATGGGGGGCAGCAGCTGTGGGCCTGGGGCTCACCTTATTCACCTGTGG
  CCCCCACAGTTTGCATTCTCTGATCACCATCTTGGGAACCTGGGCCCTCATTCAGGCCCAGCCCTGCTCC
  TGCCATGCCCTGGCTCTTGCCTGGACCTTCTCCTATCTCCTCTTCTTCCGAGCCCTCAGCCTGCTGGGCC
  TGCCCACTCCCACGCCCTTCACCAATGCTGTCCAGCTGCTGTTGACACTGAAGTTGGTGAGTCTAGCTAG
  TGAAGTCCAGGATCTGCATCTGGCTCAGAGAAAGGAAATAGCCTCCGGCTTCCACAAGGAGCCTACGCTG
  GGCCTCCTCCCTGAGGTCCCCTCTTTGATGGAGACACTCAGCTATAGCTACTGTTACGTGGGAATCATGA
  CAGGCCCATTCTTCCGCTACCGCACCTACCTGGATTGGCTGGAACAGCCCTTCCCGGAAGCCGTGCCCAG
  CCTGAGGCCCCTGCTGCGCCGCGCCTGGCCAGCCCCGCTCTTTGGCCTGCTCTTCCTGCTGTCCTCCCAT
  CTCTTCCCACTGGAAGCTGTGCGTGAGGACGCCTTCTACGCCCGCCCGCTGCCCACCCGCCTCTTCTACA
  TGATCCCGGTCTTCTTCGCCTTCCGCATGCGCTTCTACGTTGCCTGGATTGCGGCCGAGTGCGGTTGCAT
  TGCCGCGGGCTTCGGGGCCTACCCTGTGGCTGCCAAAGCCCGGGCCGGGGGCGGCCCCACCCTCCAATGC
  CCACCCCCTAGCAGTCCGGAGATTGCAGCTTCCCTGGAGTATGACTATGAGACCATCCGTAACATCGACT
  GCTATGGCACAGACTTCTGCGTGCGTGTGCGGGATGGCATGCGATACTGGAACATGACCGTGCAGTGGTG
  GCTGGCACAGTACATCTACAAGAGCGCACCTTTCTGCTCCTACGTTTTGAGGAGTGCCTGGACCATGCTG
  TTGAGTGCCTACTGGCATGGCCTCCACCCTGGTTACTACCTAAGCTTCATGACCATCCCGCTGTGCCTGG
  CTGCTGAGGGCTATTTGGAGTCAGCCTTGCGGAGACACCTGAGCCCCGGGGGCCAGAAAGCCTGGGACTG
  GGTCCACTGGTTCCTGAAGATGCGTGCCTACGACTACATGTGCATGGGCTTTGTGCTCCTTTCCATGGCT
  GACACACTCCGGTACTGGGCCTCCATCTACTTCTGGGTCCACTTTCTAGCCCTGGCCTGCTTGGGGCTGG
  GGCTGGTTTTGGGTGGGGGCAGCCCCAGCAAGAGGAAGACACCATCCCAGGCTACCAGCAGCCAAGCGAA
  GGAAAAGCTCCGGGAAGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207548 representing NM_029934
  Red=Cloning site Green=Tags(s)
  
  MTPEEWTYLMVLLISIPVGFLFKKAGPGLKRWGAAAVGLGLTLFTCGPHSLHSLITILGTWALIQAQPCS
  CHALALAWTFSYLLFFRALSLLGLPTPTPFTNAVQLLLTLKLVSLASEVQDLHLAQRKEIASGFHKEPTL
  GLLPEVPSLMETLSYSYCYVGIMTGPFFRYRTYLDWLEQPFPEAVPSLRPLLRRAWPAPLFGLLFLLSSH
  LFPLEAVREDAFYARPLPTRLFYMIPVFFAFRMRFYVAWIAAECGCIAAGFGAYPVAAKARAGGGPTLQC
  PPPSSPEIAASLEYDYETIRNIDCYGTDFCVRVRDGMRYWNMTVQWWLAQYIYKSAPFCSYVLRSAWTML
  LSAYWHGLHPGYYLSFMTIPLCLAAEGYLESALRRHLSPGGQKAWDWVHWFLKMRAYDYMCMGFVLLSMA
  DTLRYWASIYFWVHFLALACLGLGLVLGGGSPSKRKTPSQATSSQAKEKLREE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029934
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029934.3, NP_084210.2
• RefSeq Size: 2880 bp
• RefSeq ORF: 1422 bp
• Locus ID: 77582
• UniProt ID: Q8CHK3
• Cytogenetics: 7 A1
• Gene Summary: Acyltransferase which contributes to the regulation of free arachidonic acid (AA) in the cell through the remodeling of phospholipids. Mediates the conversion of lysophosphatidylinositol (1-acylglycerophosphatidylinositol or LPI) into phosphatidylinositol (1,2-diacyl-sn-glycero-3-phosphoinositol or PI) (LPIAT activity). Prefers arachidonoyl-CoA as the acyl donor (PubMed:23097495). Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle (By similarity). Required for cortical lamination during brain development (PubMed:23097495).[UniProtKB/Swiss-Prot Function]