Amotl2 (NM_019764) Mouse Tagged ORF Clone

CAT#: MG207385

Amotl2 (tGFP-tagged) - Mouse angiomotin like 2 (Amotl2)

Product Images

Circular map for MG207385

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Amotl2
• Synonyms: AW549739; C79691; Lccp; MASCOT; mKIAA0989
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207385 representing NM_019764
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGACACTGGAAGACTCCTCAGGGACAGTCCTGCACCGTCTCATCCAAGAGCAGCTGCGCTACGGCA
  ACCTGACAGAGACTCGCACTCTGCTGGCTATCCAGCAGCAGGCCCTGCGGGGTGGGGCTGGAGCTGGGGG
  CACGGGGAGCCCCCAGGCTTCCTTGGAGATCGGAGCACCCGAGGACAGTCAGGTGCTGCAGCAAGCCACC
  AGGCAGGAGCCCCAGGGCCAGGAGCATCAGGGTGGAGAGACCCACCTGGCAGAGAACAGGCTGTACCGGC
  TGTGCCCACAGCCCAGCAAAGGAGAAGAGTTGCCCACCTATGAGGAGGCCAAAGCCCATTCGCAGTACTA
  CGCAGCGCAGCAGGCAGGGTCCCGGCCGCATGTTGGGGACCGGGATCCTAGAGGAGGGGTGTCCGGAGGC
  GGCCGGCGACAGGATGAAGCTCTTCGAGAGCTGAGGCATGGCCATGTGCGCTCCTTGAGTGAACGGCTTC
  TGCAACTGTCCCTGGAAAGAAACGGTGCTCGGGTCCCCAGCCACATGAGCTCTTCCCACAGCTTCCCTCA
  GCTGGCCCGCAGCCAGCAGGGCCCCCAACCCCGAGGGCCCCCAGCTGAGGGCCCAGAGCCCCGCGGGCCA
  CCACCTCAGTACCCACACGCTGTAATGGCTCAGGAGACTGCGGCTGTCACTGACCCAAGATACCGACCCC
  GAAGCAGCCCACACTTCCAGCATGCCGAAGTCAGGATCCTGCAGGCCCAGGTACCACCGGTGTTCCTCCA
  GCAGCAGCAGTACCAGTACCTGCCGCAGCCCCAGGAGCACTCTCCACCCCTCCACCCGGCAGCTCTGGGC
  CATGGACCCCCAAGCTCCTTTGGTCCACCTGCAGTGGAGGGACCACCCAGTGCCCAGGCCACCTTGGGCA
  GTGCCCACCTGGCCCAGATGGAGACTGTACTGAGGGAGAATGCCAGGCTGCAGAGGGACAATGAGCGATT
  GCAGAGAGAGCTGGAGAGCACTTCAGAGAAGGCTGGCCGCATAGAAAAGCTGGAAAATGAAATCCAGCGG
  CTCTCTGAGGCCCACGAGAGCCTGATGAGGACCTCTTCCAAGCGTGAGGCCCTGGAGAAGACCATGAGGA
  ACAAGATGGACGGTGAGATGAGACGGTTGCAGGACTTCAACCGAGACCTTAGAGAGAGATTGGAATCGGC
  AAACCGCCACCTGGCAAGCAAGACCCAGGAAGCCCAGGCGGGCAGTCAGGACATGGTGGCGAAACTGCTT
  GCCCAGAGCTATGAGCAGCAACAGGAACAGGAGAAGCTGGAGCGGGAGATGGCACTGCTGCGTGGTGCCA
  TCGAGGACCAGCGGCGACATGCTGAACTGCTGGAGCAGGCTCTGGGCAATGCACAAAGCCGTGCCGCCCG
  GGCTGAAGAGGAGCTACGCAAAAAGCAGGCCTATGTGGAGAAGGTGGAGCGGCTGCAGCAGGCACTGGGG
  CAGTTGCAGGCTGCCTGTGAAAAGCGAGAGCAGTTGGAGCTGCGTCTGCGCACGCGCCTGGAGCAGGAAC
  TCAAAGCCTTGCGTGCACAGCAGAGGCAGACAGGCACCCTCGCAGGTGGTGGCGGCAGCCATGGTGGGTC
  CGCCGAGCTCAGTGCCCTGCGGCTGTCTGAACAGCTGCGGGAGAAGGAGGAACAGATCCTGGCTCTAGAG
  GCGGACATGACCAAGTGGGAACAGAAGTATTTGGAAGAACGGGCTATGAGGCAGTTCGCCATGGACGCGG
  CTGCCACTGCGGCTGCCCAGCGCGATACCACTCTCATCCGGCACTCCCCCCAGCCCTCGCCCAGCAGCAG
  TTTCAACGAGGGCCTGCTGCCAGGCAACCACAGGCACCAGGAGATGGAGAGCAGATTGAAGGTGCTCCAT
  GCTCAGATCCTAGAGAAGGATGCGGTGATCAAGGTCCTTCAGCAGCGCTCCAGGAAAGACCCTGGCAAGG
  CCACCCAGGGCACCCTACGGCCTGCCAAGTCGGTGCCGTCCATCTTCGCGGCTGCAGTGGGAACTCAGGG
  CTGGCAAGGGCTCGTATCCAGTGAGCGGCAAACTGATGCACGGCCAGCGGGAGACCGGGTCCCAGCAGAG
  GAGCCTCCGGCCACAGCTCCTCTCCCTGCCCACACCAAACATGGCAGCAGAGACGGGAGCACCCAGACGG
  ATGGCCCTGCAGACAACACCTCTGCCTGCTTGGCCTCAGAACCCGATGGCCTCCTGGGGTGCAACAGTAG
  CCAGAGGACACCCTCTCTGGACTCTATAGCTGCAACCAGAGTCCAGGATCTGTCAGACATGGTAGAAATA
  CTGATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207385 representing NM_019764
  Red=Cloning site Green=Tags(s)
  
  MRTLEDSSGTVLHRLIQEQLRYGNLTETRTLLAIQQQALRGGAGAGGTGSPQASLEIGAPEDSQVLQQAT
  RQEPQGQEHQGGETHLAENRLYRLCPQPSKGEELPTYEEAKAHSQYYAAQQAGSRPHVGDRDPRGGVSGG
  GRRQDEALRELRHGHVRSLSERLLQLSLERNGARVPSHMSSSHSFPQLARSQQGPQPRGPPAEGPEPRGP
  PPQYPHAVMAQETAAVTDPRYRPRSSPHFQHAEVRILQAQVPPVFLQQQQYQYLPQPQEHSPPLHPAALG
  HGPPSSFGPPAVEGPPSAQATLGSAHLAQMETVLRENARLQRDNERLQRELESTSEKAGRIEKLENEIQR
  LSEAHESLMRTSSKREALEKTMRNKMDGEMRRLQDFNRDLRERLESANRHLASKTQEAQAGSQDMVAKLL
  AQSYEQQQEQEKLEREMALLRGAIEDQRRHAELLEQALGNAQSRAARAEEELRKKQAYVEKVERLQQALG
  QLQAACEKREQLELRLRTRLEQELKALRAQQRQTGTLAGGGGSHGGSAELSALRLSEQLREKEEQILALE
  ADMTKWEQKYLEERAMRQFAMDAAATAAAQRDTTLIRHSPQPSPSSSFNEGLLPGNHRHQEMESRLKVLH
  AQILEKDAVIKVLQQRSRKDPGKATQGTLRPAKSVPSIFAAAVGTQGWQGLVSSERQTDARPAGDRVPAE
  EPPATAPLPAHTKHGSRDGSTQTDGPADNTSACLASEPDGLLGCNSSQRTPSLDSIAATRVQDLSDMVEI
  LI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019764
• ORF Size: 2316 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019764.2, NP_062738.2
• RefSeq Size: 4191 bp
• RefSeq ORF: 2319 bp
• Locus ID: 56332
• UniProt ID: Q8K371
• Cytogenetics: 9 F1
• Gene Summary: Regulates the translocation of phosphorylated SRC to peripheral cell-matrix adhesion sites. Required for proper architecture of actin filaments. Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus. Participates in angiogenesis. May play a role in the polarity, proliferation and migration of endothelial cells. Selectively promotes FGF-induced MAPK activation through SRC (By similarity).[UniProtKB/Swiss-Prot Function]