Ppp2r5c (NM_001081458) Mouse Tagged ORF Clone

CAT#: MG207212

Ppp2r5c (tGFP-tagged) - Mouse protein phosphatase 2, regulatory subunit B (B56), gamma isoform (Ppp2r5c), transcript variant 3

Product Images

Circular map for MG207212

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ppp2r5c
• Synonyms: 2610043M05Rik; 2700063L20Rik; AI060890; AW545884; C85228; D12Bwg0916e; mKIAA0044
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207212 representing NM_001081458
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGACATGTAATAAAGCGGGCAGCGGGATGGTGGTGGATGCGGCCAGCTCCAACGGGCCTTTCCAGC
  CCGTGGCCCTTCTCCACATTCGAGATGTTCCTCCTGCGGATCAAGAGAAGCTTTTTATCCAGAAGCTACG
  CCAGTGTTGTGTCCTCTTTGACTTTGTCTCTGACCCACTGAGTGACCTGAAGTGGAAGGAAGTAAAGCGC
  GCTGCGCTGAGCGAGATGGTGGAGTATATCACCCACAACCGGAACGTGATCACGGAGCCCATTTACCCCG
  AGGCCGTCCACATGTTTGCAGTTAACATGTTCCGAACCTTGCCACCTTCCTCCAATCCCACGGGAGCAGA
  ATTCGACCCAGAAGAGGATGAACCAACGTTAGAAGCAGCCTGGCCTCATCTGCAGCTTGTTTATGAATTT
  TTCTTAAGATTTTTAGAGTCTCCAGATTTCCAACCCAATATAGCAAAGAAATATATTGATCAGAAGTTTG
  TATTGCAGCTTCTAGAGCTGTTTGACAGCGAGGATCCTCGGGAGAGAGATTTTCTAAAAACCACCCTGCA
  CAGAATCTATGGGAAGTTCTTAGGCCTGCGTGCTTACATCAGGAAACAGATCAATAATATATTTTATAGG
  TTTATCTATGAGACAGAGCATCACAATGGCATAGCGGAGTTACTGGAGATCCTGGGAAGTATAATTAATG
  GATTTGCCTTACCACTGAAGGAGGAACACAAGATTTTCCTGCTGAAGGTGTTGCTGCCCTTGCACAAAGT
  GAAGTCCCTGAGTGTCTACCATCCCCAGCTGGCGTACTGTGTCGTGCAGTTTTTAGAGAAGGACAGCACC
  CTCACTGAACCAGTGGTAATGGCACTTCTCAAATACTGGCCAAAGACTCACAGTCCAAAAGAAGTAATGT
  TCTTAAATGAATTAGAAGAAATTTTAGATGTAATTGAACCATCAGAGTTTGTGAAGATCATGGAGCCTCT
  TTTCCGACAGTTAGCCAAATGTGTTTCCAGCCCTCACTTCCAGGTGGCCGAGCGGGCGCTCTATTACTGG
  AACAACGAGTACATCATGAGTTTAATCAGTGACAACGCAGCGAAGATTCTGCCCATCATGTTTCCGTCCT
  TATACCGCAACTCAAAGACCCACTGGAACAAGACAATACACGGCTTGATATACAACGCCCTGAAACTCTT
  CATGGAGATGAACCAAAAACTCTTCGATGACTGCACTCAGCAGTTCAAAGCAGAGAAACTCAAAGAGAAG
  CTAAAAATGAAAGAGCGAGAAGAAGCATGGGTTAAAATAGAAAATCTAGCCAAAGCGAATCCCCAGGTAC
  TAAAAAAGAGAGTAACTCGGGAGTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207212 representing NM_001081458
  Red=Cloning site Green=Tags(s)
  
  MLTCNKAGSGMVVDAASSNGPFQPVALLHIRDVPPADQEKLFIQKLRQCCVLFDFVSDPLSDLKWKEVKR
  AALSEMVEYITHNRNVITEPIYPEAVHMFAVNMFRTLPPSSNPTGAEFDPEEDEPTLEAAWPHLQLVYEF
  FLRFLESPDFQPNIAKKYIDQKFVLQLLELFDSEDPRERDFLKTTLHRIYGKFLGLRAYIRKQINNIFYR
  FIYETEHHNGIAELLEILGSIINGFALPLKEEHKIFLLKVLLPLHKVKSLSVYHPQLAYCVVQFLEKDST
  LTEPVVMALLKYWPKTHSPKEVMFLNELEEILDVIEPSEFVKIMEPLFRQLAKCVSSPHFQVAERALYYW
  NNEYIMSLISDNAAKILPIMFPSLYRNSKTHWNKTIHGLIYNALKLFMEMNQKLFDDCTQQFKAEKLKEK
  LKMKEREEAWVKIENLAKANPQVLKKRVTREC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081458
• ORF Size: 1356 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081458.3
• RefSeq Size: 2377 bp
• RefSeq ORF: 1359 bp
• Locus ID: 26931
• UniProt ID: Q60996
• Cytogenetics: 12 60.56 cM
• Gene Summary: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation (By similarity).[UniProtKB/Swiss-Prot Function]