Relt (NM_177073) Mouse Tagged ORF Clone

CAT#: MG206960

Relt (tGFP-tagged) - Mouse tumor necrosis factor receptor superfamily, member 19-like (Tnfrsf19l)

Product Images

Circular map for MG206960

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Relt
• Synonyms: E430021K24Rik; Tnfrsf19l
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206960 representing NM_177073
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACTCCAGGGCCTGATGATGAAGCGGACCTTGCTGTGCTGGCCCCTGTCTTGCCTCTTTGTGCTGC
  TGCCCTGGCCTCTGGCCACTCCAACACCAATAACTCCTTGGCTGTGTCCACCTGGCAAAGAGCCTGACCC
  AGATCCAGGACAGGGCACATTATGCAGAACTTGCCCCCCAGGAACCTTTTCAGCCTCATGGAACTCCTAT
  CCATGCCAGCCTCATTACCGATGCAGCCTTCAAAAGAGGCTGGAGGCCCAGGCTGGCACAGCAACTCATG
  ATACAATGTGTGGAGACTGCCAGCATGGGTGGTTTGGGCCACAGGGAGTTCCTCATGTTCCGTGTCAGCC
  ATGTTCCAAGGCACCTCCAAGTACTGGTGGCTGTGATGAGTCAGGGCGGCGGGGCCGGCGTGGCGTCGAA
  GTGGCAGCAGGTACCAGTAGCAACGGTGAACCTCGGCAGCCCGGGAATGGCACTCGGGCAGGCGGTCCTG
  AGGAGACGGCTGCCCAGTATGCAGTGATTGCCATCGTTCCTGTCTTTTGTCTCATGGGGCTTCTGGGCAT
  CCTGGTGTGCAACCTGCTCAAGCGGAAGGGCTACCATTGCACAGCCCAAAAGGAAGTTGGGCCCAGCCCT
  GGTGGAGGAGGCAGCGGGATTAATCCTGCCTATAGGACTGAAGATGCCAACGAGGACACCATTGGAGTCC
  TGGTGCGCCTGATCACAGAGAAGAAAGAGAATGCAGCGGCCCTGGAGGAGCTGTTGAAAGAATATCACAG
  CAAACAGCTGGTACAGACAAGTCACAGGCCTGTACCCAGGCTGCTGCCGGCCTCACCCAGCATACCCCAC
  ATCTGCCCGCATCACCACCACCTGCACACTGTGCAGGGCCTGGCCTCACTCTCTGGCCCCTGTTGCTCCC
  GTTGTAGCCAGAAGTGGCCAGAGGTGCTGCTGTCTCCTGAGGCAGCAGCTGCCACCACTCCTGCTCCCAC
  CCTTCTGCCTACTGCATCCAGGGCTCCCAAGGCTAGTGCCAAGCCAGGACGTCAGGGCGAGATTACCATC
  TTGTCTGTGGGCAGGTTCCGTGTGGCTCGTATTCCTGAGCAGCGGACCAGTTCATTGTTATCTGAGGTGA
  AGACCATCACGGAGGCTGGGCCTTCAGAGGGTGATCTCCCTGACTCCCCACAGCCTGGTCTTCCCCCCGA
  GCAGCGGGCACTGCTGGGAAGTGGTGGGAGCCATACTAAGTGGTTGAAGCCCCCAGCAGAGAACAAAGCT
  GAGGAGAACCGCTATGTGGTCCGGCTAAGTGAAAGCAACCTGGTCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206960 representing NM_177073
  Red=Cloning site Green=Tags(s)
  
  MSLQGLMMKRTLLCWPLSCLFVLLPWPLATPTPITPWLCPPGKEPDPDPGQGTLCRTCPPGTFSASWNSY
  PCQPHYRCSLQKRLEAQAGTATHDTMCGDCQHGWFGPQGVPHVPCQPCSKAPPSTGGCDESGRRGRRGVE
  VAAGTSSNGEPRQPGNGTRAGGPEETAAQYAVIAIVPVFCLMGLLGILVCNLLKRKGYHCTAQKEVGPSP
  GGGGSGINPAYRTEDANEDTIGVLVRLITEKKENAAALEELLKEYHSKQLVQTSHRPVPRLLPASPSIPH
  ICPHHHHLHTVQGLASLSGPCCSRCSQKWPEVLLSPEAAAATTPAPTLLPTASRAPKASAKPGRQGEITI
  LSVGRFRVARIPEQRTSSLLSEVKTITEAGPSEGDLPDSPQPGLPPEQRALLGSGGSHTKWLKPPAENKA
  EENRYVVRLSESNLVI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177073
• ORF Size: 1308 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177073.6, NP_796047.2
• RefSeq Size: 2796 bp
• RefSeq ORF: 1311 bp
• Locus ID: 320100
• UniProt ID: Q8BX43
• Cytogenetics: 7 E2
• Gene Summary: May play a role in apoptosis. Induces activation of MAPK14/p38 and MAPK8/JNK MAPK cascades, when overexpressed.[UniProtKB/Swiss-Prot Function]