Stk11 (NM_011492) Mouse Tagged ORF Clone

CAT#: MG206953

Stk11 (tGFP-tagged) - Mouse serine/threonine kinase 11 (Stk11)

Product Images

Circular map for MG206953

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Stk11
• Synonyms: AA408040; Lkb1; Par-4; R75140
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206953 representing NM_011492
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGTGGCGGACCCCGAGCCGTTGGGCCTTTTCTCCGAGGGCGAGCTGATGTCGGTGGGCATGGACA
  CCTTCATCCACCGCATCGACTCCACCGAGGTAATCTACCAGCCGCGCCGCAAACGCGCCAAGCTCATCGG
  CAAGTACCTGATGGGGGACCTGCTCGGGGAGGGCTCGTACGGCAAGGTGAAGGAGGTGCTGGACTCCGAG
  ACCTTATGCCGCAGGGCGGTCAAGATCCTCAAGAAGAAAAAGCTGCGCAGGATCCCCAATGGAGAGGCCA
  ACGTCAAGAAGGAGATCCAGCTGCTGCGGCGGCTGCGGCATCGGAATGTGATCCAGCTTGTGGACGTGCT
  GTACAATGAGGAGAAGCAGAAGATGTATATGGTGATGGAGTACTGCGTATGTGGCATGCAGGAGATGCTG
  GACAGTGTGCCGGAGAAGCGCTTCCCTGTGTGCCAAGCTCATGGGTACTTCCGCCAGCTGATTGACGGCC
  TGGAATACCTACACAGCCAGGGCATTGTTCACAAGGACATCAAGCCGGGCAACCTGCTACTCACCACCAA
  TGGCACACTCAAGATCTCCGACCTCGGTGTTGCCGAGGCCCTGCACCCTTTCGCTGTGGATGACACCTGC
  CGGACAAGCCAGGGCTCCCCGGCCTTCCAGCCTCCTGAGATTGCCAATGGACTGGACACCTTTTCAGGTT
  TCAAGGTGGACATCTGGTCAGCTGGGGTCACACTTTACAACATCACCACGGGCCTGTACCCATTTGAGGG
  GGACAATATCTACAAGCTCTTTGAGAACATTGGGAGAGGAGACTTCACCATCCCTTGTGACTGCGGCCCA
  CCACTCTCTGACCTACTCCGAGGGATGTTGGAGTATGAGCCGGCCAAGAGGTTCTCCATCCGACAGATTA
  GGCAGCACAGCTGGTTCCGGAAGAAACACCCTCTGGCTGAGGCGCTCGTACCTATCCCACCAAGCCCAGA
  CACTAAGGACCGCTGGCGCAGTATGACTGTAGTGCCCTACCTGGAGGACCTGCATGGCCGTGCGGAGGAG
  GAGGAGGAGGAAGACTTGTTTGACATTGAGGACGGCATTATCTACACCCAGGACTTCACAGTGCCTGGAC
  AGGTCCTGGAAGAGGAAGTGGGTCAGAATGGACAGAGCCACAGTTTGCCCAAGGCTGTTTGTGTGAATGG
  CACAGAGCCCCAGCTCAGCAGCAAGGTGAAGCCAGAAGGCCGACCTGGCACCGCCAACCCTGCGCGCAAG
  GTGTGCTCCAGCAACAAGATCCGCCGGCTCTCGGCCTGCAAGCAGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206953 representing NM_011492
  Red=Cloning site Green=Tags(s)
  
  MDVADPEPLGLFSEGELMSVGMDTFIHRIDSTEVIYQPRRKRAKLIGKYLMGDLLGEGSYGKVKEVLDSE
  TLCRRAVKILKKKKLRRIPNGEANVKKEIQLLRRLRHRNVIQLVDVLYNEEKQKMYMVMEYCVCGMQEML
  DSVPEKRFPVCQAHGYFRQLIDGLEYLHSQGIVHKDIKPGNLLLTTNGTLKISDLGVAEALHPFAVDDTC
  RTSQGSPAFQPPEIANGLDTFSGFKVDIWSAGVTLYNITTGLYPFEGDNIYKLFENIGRGDFTIPCDCGP
  PLSDLLRGMLEYEPAKRFSIRQIRQHSWFRKKHPLAEALVPIPPSPDTKDRWRSMTVVPYLEDLHGRAEE
  EEEEDLFDIEDGIIYTQDFTVPGQVLEEEVGQNGQSHSLPKAVCVNGTEPQLSSKVKPEGRPGTANPARK
  VCSSNKIRRLSACKQQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011492
• ORF Size: 1308 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011492.5
• RefSeq Size: 1643 bp
• RefSeq ORF: 1311 bp
• Locus ID: 20869
• UniProt ID: Q9WTK7
• Cytogenetics: 10 C1
• Gene Summary: This gene encodes a member of the serine/threonine kinase family. The encoded protein, a known tumor suppressor, activates (via phosphorylation) adenine monophosphate-activated protein kinase (AMPK) and AMPK-related kinase proteins. This upstream regulation of the AMPK pathway is thought to regulate a number of different processes, including cell metabolism, cell polarity, apoptosis and DNA damage response. Mutations in a similar gene in human have been associated with Peutz-Jeghers syndrome. Alternative splicing results in multiple transcript variants, including the S isoform which plays a potential role in spermiogenesis. [provided by RefSeq, Sep 2014]