Cbx6 (BC048240) Mouse Tagged ORF Clone

CAT#: MG206529

Cbx6 (tGFP-tagged) - Mouse chromobox homolog 6 (cDNA clone MGC:54981 IMAGE:6493113)

Product Images

Circular map for MG206529

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cbx6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206529 representing BC048240
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGTCTGCAGTAGGCGAGCGGGTCTTCGCGGCCGAATCCATCATTAAACGCCGGATCCGCAAGG
  GACGCATCGAGTACCTGGTGAAATGGAAAGGGTGGGCGATCAAGTACAGCACTTGGGAGCCAGAGGAGAA
  CATTCTGGATTCGAGGCTCATCGCAGCCTTCGAGCAGAAGGAAAGGGAACGTGAGCTGTATGGGCCCAAG
  AAGAGGGGACCTAAACCCAAAACTTTCCTCCTAAAGGCCCGGGCCCAGGCGGAGGCCCTCCGCATCAGTG
  ATGTGCATTTCTCTGTCAAGCCGAGCGCCAGCGCCTCCTCGCCCAAGCTGCACTCCAGCGCTGCGGTGCA
  CCGGCTCAAGAAAGACATCCGCCGCTGCCACCGTATGTCTCGCCGTCCCCTGCCACGACCAGACCCACAA
  GGGGGTAGCCCTGGCCTACGCCCACCCATCTCGCCCTTTTCTGAGACCGTGCGCATCATCAACCGTAAGG
  TGAAGCCCCGGGAGCCCAAGCGGAACCGCATCATCCTGAACCTGAAGGTGATTGACAAGGGTCCGGGAGG
  TGGCAGCACTGCGCAGGGCACCGGGGCACTGGCCCGCCCGAAAGTCCCCTCGCGGAACCGGGTCATTGGG
  AAGAGCAAGAAGTTCAGCGAGAGCATGCTCCGTACCCAGATCCGCCACATGAAGTTTGGCACATTTGCGC
  TCTACAAGCCCCCTCCTGCCCCTCTGGCGCCCTCTACTGCGGGCAAAGCCGATGTGGCCTCCTCGGGGCC
  TGGGCTGCTCCTGGCCACCCCAGCTGCTGCCCCCTTTGACGCACACAGCTCCAGCTCTTCCGGCTGCCCC
  TCACCCACACTGCAGTCCTCTGACCCAGACGATGCACCACCCAAGCTACTCCCGGAGACCCTGAGCCGAT
  CTGTGCCCAACTGGCGAGAGTCAGAGGTGCTCGATCTGTCCATCCCTCCTGAGGCGGCTGCCACCGGCCA
  GCGGGTGCCTCCTGATGTCACTGGTGCTGCGGACCAGGCGCTACATACAGCTCTGGAGCCCACAGGTGCT
  GGCTCCTCCGAGCCTGAGGCTGGGGACTGGCGCCCCGAGATGTCGCCATGCTCCAATGTAGTTGTCACAG
  ATGTCACCAGCAACCTCCTGACCGTGACCATCAAGGAATTCTGCAGCCCGGAGGATTTTGAGAAGGTGGC
  CGCTGGGGTGGCAGGTGCTACAGGGGGCGGGGGTGGCACTGGGCCAAGTAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206529 representing BC048240
  Red=Cloning site Green=Tags(s)
  
  MELSAVGERVFAAESIIKRRIRKGRIEYLVKWKGWAIKYSTWEPEENILDSRLIAAFEQKERERELYGPK
  KRGPKPKTFLLKARAQAEALRISDVHFSVKPSASASSPKLHSSAAVHRLKKDIRRCHRMSRRPLPRPDPQ
  GGSPGLRPPISPFSETVRIINRKVKPREPKRNRIILNLKVIDKGPGGGSTAQGTGALARPKVPSRNRVIG
  KSKKFSESMLRTQIRHMKFGTFALYKPPPAPLAPSTAGKADVASSGPGLLLATPAAAPFDAHSSSSSGCP
  SPTLQSSDPDDAPPKLLPETLSRSVPNWRESEVLDLSIPPEAAATGQRVPPDVTGAADQALHTALEPTGA
  GSSEPEAGDWRPEMSPCSNVVVTDVTSNLLTVTIKEFCSPEDFEKVAAGVAGATGGGGGTGPSK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC048240
• ORF Size: 1244 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC048240, AAH48240
• RefSeq Size: 3124 bp
• RefSeq ORF: 1244 bp
• Locus ID: 494448
• Cytogenetics: 15 E1
• Gene Summary: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Possibly contributes to the target selectivity of the PRC1 complex by binding specific regions of chromatin (By similarity). Recruitment to chromatin might occur in an H3K27me3-independent fashion (PubMed:22226355, PubMed:16537902). May have a PRC1-independent function in embryonic stem cells (PubMed:22226355).[UniProtKB/Swiss-Prot Function]