Dpagt1 (NM_007875) Mouse Tagged ORF Clone

CAT#: MG206451

Dpagt1 (tGFP-tagged) - Mouse dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)

Product Images

Circular map for MG206451

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dpagt1
• Synonyms: AU021132; Dpagt2; Gnpta; GPT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206451 representing NM_007875
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGCCTTCCCGGAGTTGCCCCTGCCGCTGCCGCTGCTGGTGAATTTGATCGGCTCGCTGTTGGGAT
  TCGTGGCTACAGTCACCCTCATCCCTGCCTTCCGTAGCCACTTTATCGCCGCGCGCCTCTGTGGCCAGGA
  CCTCAACAAGCTCAGCCAGCAGCAGATCCCAGAGTCCCAGGGAGTGATCAGCGGTGCTGTTTTCCTTATC
  ATCCTCTTCTGCTTCATCCCTTTCCCCTTCCTGAACTGCTTCGTGGAGGAGCAGTGTAAGGCATTCCCCC
  ACCATGAATTTGTGGCCCTAATAGGTGCCCTCCTTGCCATCTGCTGCATGATCTTCCTGGGGTTTGCTGA
  TGATGTCCTCAATCTCCGCTGGCGCCACAAGCTGCTGCTGCCCACAGCTGCCTCACTACCTCTCCTCATG
  GTCTACTTCACAAACTTTGGCAATACAACCATCGTGGTGCCCAAGCCCTTCCGCTGGATTCTGGGCCTGC
  ATTTGGACTTGGGGATCCTGTACTACGTCTACATGGGGCTGCTTGCAGTGTTCTGTACCAATGCCATCAA
  CATCCTGGCGGGCATTAATGGCCTAGAGGCCGGTCAGTCACTAGTCATCTCTGCTTCTATCATTGTCTTC
  AACCTGGTGGAACTGGAAGGTGATTATCGAGATGATCATATCTTTTCCCTTTACTTCATGATACCATTTT
  TCTTTACCACCTTGGGACTGCTTTACCACAACTGGTACCCGTCCCGCGTGTTTGTGGGAGACACCTTCTG
  TTACTTTGCGGGCATGACTTTTGCCGTGGTGGGGATCTTGGGACACTTCAGCAAGACCATGCTGCTCTTC
  TTTATGCCACAAGTATTCAATTTCCTCTACTCACTGCCTCAGCTCTTCCATATCATCCCCTGCCCTCGAC
  ACCGGATGCCCAGACTCAACGCAAAGACAGGCAAACTGGAAATGAGCTATTCCAAGTTCAAGACCAAGAA
  CCTCTCTTTCCTGGGCACCTTTATTTTAAAGGTAGCAGAGAACCTCCGGTTAGTGACAGTTCACCAAGGT
  GAGAGTGAGGACGGTGCCTTCACTGAGTGTAACAACATGACCCTCATCAACTTGCTACTTAAAGTCTTTG
  GGCCTATACATGAGAGAAACCTCACCCTGCTCCTGCTGCTCCTGCAGGTCCTAAGCAGCGCCGCCACCTT
  CTCCATTCGTTACCAACTCGTCCGACTCTTCTATGATGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206451 representing NM_007875
  Red=Cloning site Green=Tags(s)
  
  MWAFPELPLPLPLLVNLIGSLLGFVATVTLIPAFRSHFIAARLCGQDLNKLSQQQIPESQGVISGAVFLI
  ILFCFIPFPFLNCFVEEQCKAFPHHEFVALIGALLAICCMIFLGFADDVLNLRWRHKLLLPTAASLPLLM
  VYFTNFGNTTIVVPKPFRWILGLHLDLGILYYVYMGLLAVFCTNAINILAGINGLEAGQSLVISASIIVF
  NLVELEGDYRDDHIFSLYFMIPFFFTTLGLLYHNWYPSRVFVGDTFCYFAGMTFAVVGILGHFSKTMLLF
  FMPQVFNFLYSLPQLFHIIPCPRHRMPRLNAKTGKLEMSYSKFKTKNLSFLGTFILKVAENLRLVTVHQG
  ESEDGAFTECNNMTLINLLLKVFGPIHERNLTLLLLLLQVLSSAATFSIRYQLVRLFYDV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007875
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007875.3
• RefSeq Size: 1920 bp
• RefSeq ORF: 1233 bp
• Locus ID: 13478
• UniProt ID: P42867
• Cytogenetics: 9 24.84 cM
• Gene Summary: Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis in N-linked protein glycosylation pathway: transfers GlcNAc-1-P from UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol.[UniProtKB/Swiss-Prot Function]