St6gal1 (NM_145933) Mouse Tagged ORF Clone

CAT#: MG206325

St6gal1 (tGFP-tagged) - Mouse beta galactoside alpha 2,6 sialyltransferase 1 (St6gal1)

Product Images

Circular map for MG206325

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: St6gal1
• Synonyms: AW742324; Si; Siat1; St6G; St6gal; St6Gal-I; St6galI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206325 representing NM_145933
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCATACCAACTTGAAGAGAAAGTTCAGCTGCTTTGTCCTGGTCTTTCTCCTGTTTGCCATCATCT
  GCGTGTGGAAGAAAGGGAGCGACTATGAGGCTCTTACATTGCAAGCCAAGGTATTCCAGATGCCGAAGAG
  CCAGGAGAAAGTGGCCGTGGGGCCTGCTCCCCAGGCTGTGTTCTCAAACAGCAAACAAGACCCTAAGGAA
  GGCGTTCAGATCCTCAGTTACCCCAGGGTCACAGCCAAGGTCAAGCCACAGCCCTCCTTGCAGGTGTGGG
  ACAAGGACTCCACATACTCAAAACTTAACCCCAGGCTGCTGAAGATCTGGAGGAACTATCTGAACATGAA
  TAAATATAAAGTGTCCTACAAGGGGCCGGGACCAGGAGTCAAGTTCAGCGTAGAGGCGCTGCGCTGCCAC
  CTTCGAGACCACGTGAATGTGTCTATGATAGAGGCCACAGATTTTCCCTTCAACACCACTGAATGGGAGG
  GTTACCTGCCCAAGGAGAACTTCAGAACCAAGGCTGGGCCTTGGCATAAGTGTGCCGTCGTGTCTTCTGC
  AGGATCTCTGAAGAACTCCCAGCTGGGTCGAGAGATTGATAATCATGATGCGGTCCTGAGGTTTAATGGG
  GCACCTACAGACAACTTCCAACAGGATGTGGGCACAAAAACTACCATCCGCCTAGTGAACTCTCAGTTAG
  TCACCACAGAAAAGCGCTTCCTGAAGGACAGTTTGTACACCGAAGGAATCCTGATTCTGTGGGACCCATC
  TGTGTATCATGCAGACATTCCGCAGTGGTATCAGAAGCCAGACTACAACTTCTTCGAAACCTATAAGAGT
  TACCGAAGGCTTCACCCCAGCCAGCCTTTTTACATCCTCAAGCCCCAGATGCCATGGGAACTATGGGACA
  TCATTCAGGAAATCTCTCCAGATCTGATTCAGCCGAATCCCCCATCCTCCGGCATGCTGGGTATCATCAT
  TATGATGACGCTGTGTGACCAAGTTGATATTTACGAGTTCCTCCCATCCAAGCGCAAGACAGATGTGTGC
  TACTATCACCAGAAGTTCTTTGACAGCGCCTGCACGATGGGTGCCTACCATCCGCTCCTCTTCGAGAAGA
  ATATGGTGAAGCATCTCAATGAGGGAACAGATGAAGACATTTATTTGTTTGGGAAAGCTACCCTGTCTGG
  CTTCCGGAACAATCGCTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206325 representing NM_145933
  Red=Cloning site Green=Tags(s)
  
  MIHTNLKRKFSCFVLVFLLFAIICVWKKGSDYEALTLQAKVFQMPKSQEKVAVGPAPQAVFSNSKQDPKE
  GVQILSYPRVTAKVKPQPSLQVWDKDSTYSKLNPRLLKIWRNYLNMNKYKVSYKGPGPGVKFSVEALRCH
  LRDHVNVSMIEATDFPFNTTEWEGYLPKENFRTKAGPWHKCAVVSSAGSLKNSQLGREIDNHDAVLRFNG
  APTDNFQQDVGTKTTIRLVNSQLVTTEKRFLKDSLYTEGILILWDPSVYHADIPQWYQKPDYNFFETYKS
  YRRLHPSQPFYILKPQMPWELWDIIQEISPDLIQPNPPSSGMLGIIIMMTLCDQVDIYEFLPSKRKTDVC
  YYHQKFFDSACTMGAYHPLLFEKNMVKHLNEGTDEDIYLFGKATLSGFRNNRC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145933
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145933.4
• RefSeq Size: 4529 bp
• RefSeq ORF: 1212 bp
• Locus ID: 20440
• UniProt ID: Q64685
• Cytogenetics: 16 14.03 cM
• Gene Summary: This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on chromosome 15. [provided by RefSeq, Nov 2011]