Ttc4 (NM_028209) Mouse Tagged ORF Clone

CAT#: MG206039

Ttc4 (tGFP-tagged) - Mouse tetratricopeptide repeat domain 4 (Ttc4)

Product Images

Circular map for MG206039

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ttc4
• Synonyms: 2810002P21Rik; AF007005; L62
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206039 representing NM_028209
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGTTCTGAGCCTGAGCCTACAGAGGACGCTTCCATGGACGCGTTCCTGGAAAAGTTTCAGAGCC
  AGCCTTACCGCGGCGGCTTCCGCGAGGATCAGTGGGAGGAGGAATTTGACAAGATTCCCCTATTTATGAA
  AAAAGCGCCATCAGAAATTGACCCCGAGGAGTTTCCTGACTTGGCCTGCCTCCAGTCGATGATTTTTGAT
  GATGATCGATATCCAGAAGAGCAAGCCAAGACCTATAAAGATGAGGGCAATGACTACTTTAAAGAAAAAG
  ACTATAAGAAAGCTGTGCTTTCATACAGTGAAGGGTTGAAGAAGAAGTGTGCAGACCCTGATTTGAATGC
  TGTCCTGTATACCAACCGGGCAGCAGCACAGTACTATCTCGGCAATGTTCGTTCTTCCCTCAATGATGTG
  CTGGCTGCCAAGAAGCTAAAGCCCGGTCACCTGAAAGCCATCATAAGAGGTGCCCTGTGCCATCTTGAAC
  TGAAACACTTTGCTGAGGCTGTGAACTGGTGTGATGAGGGACTACAAATTGATGCCAAAGAGAAGAAACT
  TCTAGAAATAAGGGCAAAAGCAGACAAGCTAAAGAGGATGGAAGAGAGGGATCTGAGGAAGGCAAAGTTG
  AAAGAAAAGAAGGAACAGCATCAGAATGAGGCTCTGCTCCAGGCCATCAAGGCTAGGAACATCAGGCTTG
  TCTCTGAATCAGCCGGTGAGGATGAAGATTCAGCCTCCAATGGTCCAGCTGAGATACTCCTGGATGGGCT
  CAGCTCTGAGAACCCCTATGGAGCCAGGCTAAGTATAGATGACCAGGGCAGGCTGAGCTGGCCTGTGCTG
  TTCCTGTACCCGGAGTACGCGCAATCAGACTTCATCTCTGCTTTTCATGAGGACACCAGGTTTATTGACC
  ATCTAATGGCGATGTTTAGTGAAGCACCCTCCTGGGACTCAGAGCACAAGTACCACCCAGAGAATCTGGA
  GGTCTACTTCGAGGATGAGGACAGGGCGGAACTATACCAGGTGTCTCCCGATAGCACCCTGTTGCAGGTG
  CTGCAGCACCCCAGGTGCTGCGTGAAGGCCCTTACCCCGGCATTCCTGGTCTGTGTAGGATCCTCGCCTT
  TCTGTAGGAATTACCTCCAGGGGAAGAAGGTGCACAGG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206039 representing NM_028209
  Red=Cloning site Green=Tags(s)
  
  MESSEPEPTEDASMDAFLEKFQSQPYRGGFREDQWEEEFDKIPLFMKKAPSEIDPEEFPDLACLQSMIFD
  DDRYPEEQAKTYKDEGNDYFKEKDYKKAVLSYSEGLKKKCADPDLNAVLYTNRAAAQYYLGNVRSSLNDV
  LAAKKLKPGHLKAIIRGALCHLELKHFAEAVNWCDEGLQIDAKEKKLLEIRAKADKLKRMEERDLRKAKL
  KEKKEQHQNEALLQAIKARNIRLVSESAGEDEDSASNGPAEILLDGLSSENPYGARLSIDDQGRLSWPVL
  FLYPEYAQSDFISAFHEDTRFIDHLMAMFSEAPSWDSEHKYHPENLEVYFEDEDRAELYQVSPDSTLLQV
  LQHPRCCVKALTPAFLVCVGSSPFCRNYLQGKKVHR
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028209
• ORF Size: 2566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028209.2, NP_082485.1
• RefSeq Size: 2085 bp
• RefSeq ORF: 1161 bp
• Locus ID: 72354
• UniProt ID: Q8R3H9
• Cytogenetics: 4 C7
• Gene Summary: May act as a co-chaperone for HSP90AB1 (By similarity).[UniProtKB/Swiss-Prot Function]