Sppl3 (NM_029012) Mouse Tagged ORF Clone

CAT#: MG205992

Sppl3 (tGFP-tagged) - Mouse signal peptide peptidase 3 (Sppl3)

Product Images

Circular map for MG205992

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sppl3
• Synonyms: 4833416I09Rik; Psl4; Usmg3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205992 representing NM_029012
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAACAGACCTATTCGTGGGCCTATTCCCTGGTGGACTCCAGTCAAGTGTCGACATTTCTGATAT
  CCATTCTTCTTATAGTCTATGGTAGTTTCAGGTCTCTTAATATGGACTTTGAAAACCAAGATAAGGAGAA
  AGACAGCAACAGTTCTTCTGGCTCTTTCAATGGCAACAGCACCAATAACAGCATCCAGACCATTGATTCC
  ACCCAAGCACTGTTCCTCCCGATTGGAGCGTCTGTCTCTCTCCTCGTCATGTTCTTCTTCTTTGATTCAG
  TTCAAGTCGTTTTCACAATATGTACAGCAGTTCTTGCAACAATAGCTTTTGCTTTTCTTCTTCTCCCGAT
  GTGCCAGTATTTAACAAGGCCCTGCTCACCTCAGAACAAGATTTCCTTCGGTTGCTGTGGGCGTTTCACT
  GCTGCCGAGCTGCTGTCGTTCTCCCTGTCTGTCATGCTCGTCCTCATCTGGGTTCTCACTGGCCACTGGC
  TTCTCATGGATGCTCTGGCCATGGGTCTCTGTGTTGCCATGATCGCCTTCGTCCGCCTGCCAAGCCTCAA
  GGTTTCCTGCCTGCTTCTCTCAGGGCTTCTCATCTACGATGTCTTCTGGGTGTTCTTCTCAGCCTACATC
  TTCAACAGTAATGTCATGGTGAAAGTGGCCACACAGCCAGCTGACAATCCCCTCGACGTTCTGTCCAGGA
  AGCTCCACCTGGGACCCAATGTGGGGCGTGATGTTCCTCGCCTGTCTTTGCCTGGAAAATTGGTCTTCCC
  AAGCTCCACTGGCAGTCACTTCTCTATGCTGGGCATCGGGGACATTGTGATGCCCGGCCTCCTGTTATGC
  TTTGTTCTTCGCTATGACAACTACAAGAAACAAGCCAGTGGTGACTCGTGTGGGGCCCCTGGCCCAGCCA
  ATATCTCTGGGCGCATGCAGAAGGTCTCCTACTTCCACTGCACCCTCATCGGGTACTTTGTAGGTCTGCT
  CACTGCGACTGTGGCGTCTCGCATCCACCGAGCTGCCCAGCCAGCTCTCCTCTACTTGGTGCCATTTACC
  CTATTGCCACTCCTCACCATGGCCTACCTAAAGGGTGACTTACGGAGGATGTGGTCTCAGCCCTTCCACT
  CCAAGTCCAGCAGCTCCCGGTTCCTGGAAGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205992 representing NM_029012
  Red=Cloning site Green=Tags(s)
  
  MAEQTYSWAYSLVDSSQVSTFLISILLIVYGSFRSLNMDFENQDKEKDSNSSSGSFNGNSTNNSIQTIDS
  TQALFLPIGASVSLLVMFFFFDSVQVVFTICTAVLATIAFAFLLLPMCQYLTRPCSPQNKISFGCCGRFT
  AAELLSFSLSVMLVLIWVLTGHWLLMDALAMGLCVAMIAFVRLPSLKVSCLLLSGLLIYDVFWVFFSAYI
  FNSNVMVKVATQPADNPLDVLSRKLHLGPNVGRDVPRLSLPGKLVFPSSTGSHFSMLGIGDIVMPGLLLC
  FVLRYDNYKKQASGDSCGAPGPANISGRMQKVSYFHCTLIGYFVGLLTATVASRIHRAAQPALLYLVPFT
  LLPLLTMAYLKGDLRRMWSQPFHSKSSSSRFLEV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029012
• ORF Size: 1152 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029012.2, NP_083288.2
• RefSeq Size: 2817 bp
• RefSeq ORF: 1155 bp
• Locus ID: 74585
• UniProt ID: Q9CUS9
• Cytogenetics: 5 F
• Gene Summary: Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane protein substrates in or close to their luminal transmembrane domain boundaries. Acts like a sheddase by mediating the proteolytic release and secretion of active site-containing ectodomains of glycan-modifiying glycosidase and glycosyltransferase enzymes such as MGAT5, B4GAT1 and B4GALT1 (PubMed:25354954, PubMed:25827571). Plays a role in the regulation of cellular glycosylation processes (PubMed:25354954). Required to link T-cell antigen receptor (TCR) and calcineurin-NFAT signaling cascades in lymphocytes by promoting the association of STIM1 and ORAI1 during store-operated calcium entry (SOCE) in a protease-independent manner (PubMed:25384971).[UniProtKB/Swiss-Prot Function]