2410091C18Rik (BC108350) Mouse Tagged ORF Clone

CAT#: MG205976

2410091C18Rik (tGFP-tagged) - Mouse RIKEN cDNA 2410091C18 gene (cDNA clone MGC:117993 IMAGE:4220568)

Product Images

Circular map for MG205976

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: 2410091C18Rik
• Synonyms: 2410091C18Rik; AL033374; PRO1853
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205976 representing BC108350
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCTTTGGTGAGACGCTGCGTGGCCCGCGCGGGCCTTCCCTGTATTTGGAGAGGGAAATGCTACA
  GCTCCGGGAATGAGCCGGCAGAAAGCAACCAGGTGACACCGATGCTGCGGCATCTTATGTACAAAATAAA
  GTCTACAGGTCCCATCACTGTGGCCGAGTACATGAAGGAGGTTTTGACTAACCCAGCCAAGGGATATTAT
  GTGCATCAGGATATGCTAGGAGAAAAAGGAGACTTTATTACTTCACCTGAAATAAGCCAAATCTTCGGGG
  AGCTACTAGGGGTGTGGTTTGTTAGTGAATGGATAGCCTCTGGAAAAAGCCCAGCATTCCAGCTGGTGGA
  ACTTGGCCCTGGTCGGGGCACCCTCACAGCAGATATTTTGAGGGTGTTCAGTCAGCTTGGGTCTGTGCTA
  AAAACCTGTGCCATCTCAATACACCTTGTAGAGGTGAGCCAGAAACTAAGTGAGATCCAAGCACTGACAC
  TGGCTGAGGAGAAGGTCCCATTGGAGAGAGACGCTGAATCCCTGGTGTACATGAAAGGTGTCACAAAGTC
  TGGGATTCCAATCTCCTGGTACCGAGACCTGAAGGATGTTCCTGAAGGATACAGCTTGTACCTCGCCCAT
  GAGTTTTTTGATGTTCTTCCTGTGCATAAATTTCAGAAAACACCACGCGGATGGAGAGAAGTGTTTGTTG
  ATGTTGATCCACAAGCTTCTGATAAACTGAGGTTCGTCTTGGCGCCTTGTGCGACCCCTGCAGAAGCCTT
  CATTCAACGTGATGAAAGGAGGGAACATGTGGAAGTATGTCCCGATGCTGGGGTTATCATCCAGGAACTG
  TCCCAGCGCATTGCGTCAACTGGAGGTGCAGCCCTGATTGCGGATTACGGTCACGATGGCACAAAGACAG
  ACACCTTGAGAGGGTTTTATGGACACCAGCTTCACGATGTCTTAATCGCTCCTGGAACAGCAGACCTGAC
  AGCTGATGTGGACTTCAGTTACCTGCACAGAATGGCACAAGGAAAAGTAGCCTCTCTGGGTCCAGTGGAA
  CAGCGAACATTTTTAAAAAACATGGGCATTGATGTCCGGCTGAAGGTAGTGGTTTATCTCAGTAACACTA
  AACCTCTTAATTTCGGAAATTATTTAAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205976 representing BC108350
  Red=Cloning site Green=Tags(s)
  
  MNALVRRCVARAGLPCIWRGKCYSSGNEPAESNQVTPMLRHLMYKIKSTGPITVAEYMKEVLTNPAKGYY
  VHQDMLGEKGDFITSPEISQIFGELLGVWFVSEWIASGKSPAFQLVELGPGRGTLTADILRVFSQLGSVL
  KTCAISIHLVEVSQKLSEIQALTLAEEKVPLERDAESLVYMKGVTKSGIPISWYRDLKDVPEGYSLYLAH
  EFFDVLPVHKFQKTPRGWREVFVDVDPQASDKLRFVLAPCATPAEAFIQRDERREHVEVCPDAGVIIQEL
  SQRIASTGGAALIADYGHDGTKTDTLRGFYGHQLHDVLIAPGTADLTADVDFSYLHRMAQGKVASLGPVE
  QRTFLKNMGIDVRLKVVVYLSNTKPLNFGNYLN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC108350
• ORF Size: 1151 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC108350, AAI08351
• RefSeq Size: 2640 bp
• RefSeq ORF: 1151 bp
• Locus ID: 73694
• Cytogenetics: 17 E3
• Gene Summary: Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). Acts by mediating symmetric dimethylation of 'Arg-118' of NDUFS2 after it assembles into the complex I, stabilizing the early intermediate complex.[UniProtKB/Swiss-Prot Function]