Gpr85 (NM_145066) Mouse Tagged ORF Clone

CAT#: MG205711

Gpr85 (tGFP-tagged) - Mouse G protein-coupled receptor 85 (Gpr85)

Product Images

Circular map for MG205711

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gpr85
• Synonyms: 2900026B03Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205711 representing NM_145066
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAACTATAGCCATGCAGCCGACAACATTTTGCAAAATCTCTCGCCTCTAACAGCCTTTCTGAAAC
  TGACTTCCCTGGGTTTCATAATAGGAGTCAGCGTTGTGGGCAACCTTCTGATCTCCATTTTGCTAGTGAA
  AGATAAGACCTTGCATAGAGCTCCTTACTACTTCCTGCTGGATCTGTGCTGCTCAGACATCCTCAGATCT
  GCAATTTGTTTTCCATTTGTATTCAACTCTGTCAAAAATGGCTCCACCTGGACTTACGGGACTCTGACTT
  GCAAAGTGATTGCCTTCCTGGGGGTTTTGTCCTGTTTCCACACTGCCTTCATGCTCTTCTGCATCAGCGT
  CACCAGATACTTAGCCATCGCCCATCACCGCTTCTATACAAAGAGGCTGACCTTTTGGACGTGTTTGGCT
  GTGATCTGCATGGTGTGGACTCTGTCCGTGGCCATGGCATTCCCCCCAGTTTTAGATGTAGGCACCTACT
  CCTTCATTAGGGAGGAGGATCAATGCACCTTCCAACACCGCTCCTTCAGGGCTAACGATTCCCTAGGATT
  TATGCTGCTCCTTGCTCTCATCCTCCTAGCCACACAGCTTGTCTACCTCAAGCTGATATTTTTTGTCCAC
  GATCGAAGGAAAATGAAGCCAGTCCAGTTTGTAGCAGCAGTGAGTCAGAACTGGACCTTTCATGGCCCTG
  GAGCTAGCGGCCAGGCAGCTGCCAATTGGCTAGCAGGATTTGGAAGGGGTCCCACACCACCCACCTTGCT
  GGGCATCAGGCAAAATGCGAATACCACAGGCAGAAGGCGGCTATTGGTCTTGGATGAGTTCAAAATGGAG
  AAAAGAATCAGCAGAATGTTTTATATAATGACTTTCCTCTTCCTAACCTTGTGGGGTCCCTACCTGGTGG
  CCTGCTATTGGAGAGTTTTTGCACGAGGGCCTGTAGTACCAGGGGGATTTCTAACAGCCGCTGTCTGGAT
  GAGTTTCGCCCAAGCAGGAATCAATCCCTTTGTCTGCATTTTCTCCAACAGGGAGCTGAGGCGCTGTTTC
  AGCACAACCCTTCTTTACTGCAGAAAATCCAGGTTACCAAGGGAACCTTACTGTGTTATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205711 representing NM_145066
  Red=Cloning site Green=Tags(s)
  
  MANYSHAADNILQNLSPLTAFLKLTSLGFIIGVSVVGNLLISILLVKDKTLHRAPYYFLLDLCCSDILRS
  AICFPFVFNSVKNGSTWTYGTLTCKVIAFLGVLSCFHTAFMLFCISVTRYLAIAHHRFYTKRLTFWTCLA
  VICMVWTLSVAMAFPPVLDVGTYSFIREEDQCTFQHRSFRANDSLGFMLLLALILLATQLVYLKLIFFVH
  DRRKMKPVQFVAAVSQNWTFHGPGASGQAAANWLAGFGRGPTPPTLLGIRQNANTTGRRRLLVLDEFKME
  KRISRMFYIMTFLFLTLWGPYLVACYWRVFARGPVVPGGFLTAAVWMSFAQAGINPFVCIFSNRELRRCF
  STTLLYCRKSRLPREPYCVI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145066
• ORF Size: 1110 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145066.5
• RefSeq Size: 2971 bp
• RefSeq ORF: 1113 bp
• Locus ID: 64450
• UniProt ID: P60894
• Cytogenetics: 6 A1
• Gene Summary: Orphan receptor.[UniProtKB/Swiss-Prot Function]