Kcnab2 (NM_010598) Mouse Tagged ORF Clone

CAT#: MG205641

Kcnab2 (tGFP-tagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, beta member 2 (Kcnab2)

Product Images

Circular map for MG205641

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kcnab2
• Synonyms: F5; I2rf5; Kcnb3; kv-beta-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205641 representing NM_010598
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATCCGGAATCAACCACGGGGTCCCCAGCTCGACTCTCCCTGCGGCAGACAGGCTCCCCCGGGATGA
  TCTACAGTACTCGTTATGGGAGTCCCAAAAGACAGCTCCAGTTTTACAGGAATCTGGGCAAATCTGGCCT
  TCGGGTCTCCTGCCTGGGGCTTGGAACATGGGTGACCTTCGGGGGCCAGATCACGGATGAGATGGCAGAG
  CACCTAATGACCTTGGCCTACGATAATGGCATCAACCTGTTCGATACGGCGGAGGTCTACGCTGCTGGAA
  AAGCTGAAGTGGTATTAGGGAACATCATTAAGAAGAAGGGATGGAGACGGTCCAGCCTTGTCATCACCAC
  CAAGATCTTCTGGGGTGGAAAAGCGGAGACTGAGAGAGGCCTTTCCAGGAAGCACATAATTGAAGGACTG
  AAAGCGTCCCTGGAGCGGCTGCAGCTGGAGTACGTGGATGTGGTTTTTGCCAACCGCCCAGACCCCAACA
  CGCCCATGGAAGAGACCGTGCGGGCCATGACCCATGTCATCAACCAGGGGATGGCCATGTACTGGGGCAC
  ATCACGCTGGAGCTCCATGGAGATCATGGAGGCCTACTCGGTGGCTCGGCAGTTCAACCTGATCCCGCCC
  ATCTGCGAGCAAGCGGAATATCACATGTTCCAGAGGGAGAAGGTGGAGGTCCAGCTGCCAGAGCTGTTCC
  ACAAGATAGGAGTAGGTGCCATGACCTGGTCCCCTCTGGCGTGCGGCATCGTCTCAGGGAAGTATGACAG
  CGGGATCCCACCCTACTCCAGAGCCTCCCTGAAGGGCTACCAGTGGTTGAAGGACAAGATCCTGAGTGAG
  GAGGGTCGCCGCCAGCAGGCCAAGCTGAAGGAACTGCAGGCCATTGCCGAACGCCTGGGCTGCACCCTAC
  CCCAGCTGGCCATAGCCTGGTGCCTGAGGAATGAGGGTGTCAGCTCCGTGCTTCTGGGTGCTTCCAATGC
  AGAACAACTTATGGAGAACATTGGAGCAATACAGGTCCTTCCAAAATTGTCGTCTTCCATCGTCCACGAG
  ATCGACAGCATTCTGGGCAATAAACCCTACAGCAAAAAGGACTATAGATCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205641 representing NM_010598
  Red=Cloning site Green=Tags(s)
  
  MYPESTTGSPARLSLRQTGSPGMIYSTRYGSPKRQLQFYRNLGKSGLRVSCLGLGTWVTFGGQITDEMAE
  HLMTLAYDNGINLFDTAEVYAAGKAEVVLGNIIKKKGWRRSSLVITTKIFWGGKAETERGLSRKHIIEGL
  KASLERLQLEYVDVVFANRPDPNTPMEETVRAMTHVINQGMAMYWGTSRWSSMEIMEAYSVARQFNLIPP
  ICEQAEYHMFQREKVEVQLPELFHKIGVGAMTWSPLACGIVSGKYDSGIPPYSRASLKGYQWLKDKILSE
  EGRRQQAKLKELQAIAERLGCTLPQLAIAWCLRNEGVSSVLLGASNAEQLMENIGAIQVLPKLSSSIVHE
  IDSILGNKPYSKKDYRS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010598
• ORF Size: 1101 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010598.4
• RefSeq Size: 3571 bp
• RefSeq ORF: 1104 bp
• Locus ID: 16498
• UniProt ID: P62482
• Cytogenetics: 4 83.08 cM
• Gene Summary: Cytoplasmic potassium channel subunit that modulates the characteristics of the channel-forming alpha-subunits (PubMed:8576199). Contributes to the regulation of nerve signaling, and prevents neuronal hyperexcitability (PubMed:11825900, PubMed:21209188). Promotes expression of the pore-forming alpha subunits at the cell membrane, and thereby increases channel activity (By similarity). Promotes potassium channel closure via a mechanism that does not involve physical obstruction of the channel pore (PubMed:8576199). Modulates the functional properties of KCNA4 (By similarity). Modulates the functional properties of KCNA5 (PubMed:8576199). Enhances KCNB2 channel activity (PubMed:8824288). Modulates the functional properties of KCNA5 (PubMed:8576199). Binds NADPH and has NADPH-dependent aldoketoreductase activity (By similarity). Has broad substrate specificity and can catalyze the reduction of methylglyoxal, 9,10-phenanthrenequinone, prostaglandin J2, 4-nitrobenzaldehyde, 4-nitroacetophenone and 4-oxo-trans-2-nonenal (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function]