Chia (BC011134) Mouse Tagged ORF Clone

CAT#: MG205616

Chia (tGFP-tagged) - Mouse chitinase, acidic (cDNA clone MGC:18771 IMAGE:4165150)

Product Images

Circular map for MG205616

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Chia
• Synonyms: YNL, AMCase
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205616 representing BC011134
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTCCACTTCTCAGAACCGCCAGACCTTCATTACCTCAGTCATCAAATTTCTGCGTCAGTATGGGT
  TTGATGGACTGGACCTGGACTGGGAATACCCAGGCTCACGTGGGAGCCCTCCTCAGGACAAGCATCTCTT
  CACTGTCCTGGTGAAGGAAATGCGTGAAGCTTTTGAGCAGGAGGCTATTGAGAGCAACAGGCCCAGACTG
  ATGGTTACTGCTGCTGTAGCTGGTGGGATTTCCAACATCCAGGCTGGCTATGAGATCCCTGAACTTTCTA
  AGTACCTGGATTTCATCCATGTCATGACATATGACCTCCATGGCTCCTGGGAGGGCTACACTGGGGAGAA
  TAGTCCTCTTTACAAATACCCTACTGAGACTGGTAGCAATGCCTACCTCAATGTGGATTATGTCATGAAC
  TATTGGAAGAACAATGGAGCCCCAGCTGAGAAGCTCATTGTTGGATTCCCAGAGTATGGACACACCTTCA
  TCCTGAGAAACCCCTCTGATAATGGAATTGGTGCCCCTACCTCTGGTGATGGCCCTGCTGGGCCCTATAC
  CAGACAGGCTGGGTTCTGGGCCTACTATGAGATTTGCACCTTTCTGAGAAGTGGAGCCACTGAGGTCTGG
  GATGCCTCCCAAGAAGTGCCCTATGCCTATAAGGCCAACGAGTGGCTTGGCTATGACAATATCAAGAGCT
  TCAGTGTTAAGGCTCAGTGGCTTAAGCAGAACAATTTTGGAGGTGCCATGATCTGGGCCATTGACCTTGA
  TGACTTCACTGGCTCTTTCTGTGATCAGGGAAAATTTCCTCTGACTTCTACTTTGAACAAAGCCCTTGGC
  ATATCCACTGAAGGTTGCACAGCTCCTGACGTGCCTTCCGAGCCAGTGACTACTCCTCCAGGAAGTGGGA
  GTGGGGGTGGAAGCTCCGGAGGAAGCTCTGGAGGCAGTGGATTCTGTGCCGACAAAGCAGATGGCCTCTA
  CCCTGTGGCAGATGACAGAAATGCTTTTTGGCAGTGCATCAATGGAATCACATACCAGCAGCATTGTCAA
  GCAGGGCTTGTTTTTGATACCAGCTGTAATTGCTGCAACTGGCCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205616 representing BC011134
  Red=Cloning site Green=Tags(s)
  
  MVSTSQNRQTFITSVIKFLRQYGFDGLDLDWEYPGSRGSPPQDKHLFTVLVKEMREAFEQEAIESNRPRL
  MVTAAVAGGISNIQAGYEIPELSKYLDFIHVMTYDLHGSWEGYTGENSPLYKYPTETGSNAYLNVDYVMN
  YWKNNGAPAEKLIVGFPEYGHTFILRNPSDNGIGAPTSGDGPAGPYTRQAGFWAYYEICTFLRSGATEVW
  DASQEVPYAYKANEWLGYDNIKSFSVKAQWLKQNNFGGAMIWAIDLDDFTGSFCDQGKFPLTSTLNKALG
  ISTEGCTAPDVPSEPVTTPPGSGSGGGSSGGSSGGSGFCADKADGLYPVADDRNAFWQCINGITYQQHCQ
  AGLVFDTSCNCCNWP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC011134
• ORF Size: 1097 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC011134, AAH11134
• RefSeq Size: 1538 bp
• RefSeq ORF: 1097 bp
• Locus ID: 81600
• Cytogenetics: 3 F2.2
• Gene Summary: Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding. Presence in saliva and gastric juice suggests a function as a digestive enzyme.[UniProtKB/Swiss-Prot Function]