Lmcd1 (NM_144799) Mouse Tagged ORF Clone

CAT#: MG205613

Lmcd1 (tGFP-tagged) - Mouse LIM and cysteine-rich domains 1 (Lmcd1)

Product Images

Circular map for MG205613

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lmcd1
• Synonyms: AW455500; AW553074; dyxin
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205613 representing NM_144799
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAAGTGGCTAAGGACCTCAACCCTGGAGTGCAAAAGATGTCCTTGGGCCAGCAGCAGTCCGCAA
  GAGGAGTGGCTTGTCTGCGGTGCAAGGGAATGTGCTCCGGCTTCGAGCCTCACTCGTGGAGGAAAATATG
  CAAGTCTTGTAAATGCAGCCAGGAGGAGCACTGCCTAAGCTCTGACCTGGACGATGATCGGAAAATTGGG
  CGCCTGCTGATGGACTCCAAGTATGCCACTCTCACCGCCAGGGTGAAAGGCGGAGACGGCATCCGCATCT
  ATAAGAGGAACCGCATGATCATGACCAACCCCATCGCCACCGGCAAAGACCCCACCTTTGACACTATCAC
  CTACGAGTGGGCTCCCCCAGGAGTCACCCAGAAACTGGGCCTGCAGTACATGGAACTCATCCCCAAGGAG
  AGGCAGCCTGTGACAGGCACTGAGGGTGCCCTCTACCGCCGACGCCAGCTCATGCATCAGCTCCCTATCT
  ATGACCAGGACCCCTCTCGATGCCGTGGACTTGTGGAGAATGAGCTGAAAGCTATGGAGGAGTTCGTCAA
  GCACTACAAGAGCGAGGCCCTTGGGGTGGGCGAGGTGGCCCTCCCAGGGCAGGGAGGCTTGCCCAAGGAA
  GAAAACAAGACACAGGAAAAGCCAGAGGGCACAGAGACCACTGCCCCAACCACCAATGGCAGCCTGGGTG
  ATCCATCCAAAGAAGTGGAATACGTCTGTGAGCTCTGCAAGGGAGCAGCCCCAGTTGACAGCCCTGTGGT
  CTACGCTGACAGGGCTGGATACAGCAAACAGTGGCACCCCACGTGCTTTCAGTGTATCAAGTGCTCAGAG
  CCACTGGTGGACCTCATCTACTTCTGGAAGGATGGTGCACCCTGGTGTGGCCGCCACTACTGTGAGAGTG
  TGCGGCCCCGGTGCTCAGGCTGTGATGAGATCATCTTCTCAGAAGACTACCAGCGTGTGGAAGACCTGGC
  CTGGCATCGGAAGCACTTCATTTGCGAGGGCTGTGAGCAGCTGCTGAGCGGCCGTGCGTACATCGTCACC
  AAGGGTCAGCTCCTGTGCCCCACATGCAGCAAGTCAAAACGCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205613 representing NM_144799
  Red=Cloning site Green=Tags(s)
  
  MAKVAKDLNPGVQKMSLGQQQSARGVACLRCKGMCSGFEPHSWRKICKSCKCSQEEHCLSSDLDDDRKIG
  RLLMDSKYATLTARVKGGDGIRIYKRNRMIMTNPIATGKDPTFDTITYEWAPPGVTQKLGLQYMELIPKE
  RQPVTGTEGALYRRRQLMHQLPIYDQDPSRCRGLVENELKAMEEFVKHYKSEALGVGEVALPGQGGLPKE
  ENKTQEKPEGTETTAPTTNGSLGDPSKEVEYVCELCKGAAPVDSPVVYADRAGYSKQWHPTCFQCIKCSE
  PLVDLIYFWKDGAPWCGRHYCESVRPRCSGCDEIIFSEDYQRVEDLAWHRKHFICEGCEQLLSGRAYIVT
  KGQLLCPTCSKSKRS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144799
• ORF Size: 1095 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144799.2
• RefSeq Size: 1695 bp
• RefSeq ORF: 1098 bp
• Locus ID: 30937
• UniProt ID: Q8VEE1
• Cytogenetics: 6 E3
• Gene Summary: Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter. Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway.[UniProtKB/Swiss-Prot Function]