Cxcr4 (NM_009911) Mouse Tagged ORF Clone

CAT#: MG205485

Cxcr4 (tGFP-tagged) - Mouse chemokine (C-X-C motif) receptor 4 (Cxcr4)

Product Images

Circular map for MG205485

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cxcr4
• Synonyms: b2b220Clo; CD184; Cmkar4; LESTR; PB-CKR; PBSF/SDF-1; Sdf1r
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205485 representing NM_009911
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCGATCAGTGTGAGTATATACACTTCTGATAACTACTCTGAAGAAGTGGGGTCTGGAGACTATG
  ACTCCAACAAGGAACCCTGCTTCCGGGATGAAAACGTCCATTTCAATAGGATCTTCCTGCCCACCATCTA
  CTTCATCATCTTCTTGACTGGCATAGTCGGCAATGGATTGGTGATCCTGGTCATGGGTTACCAGAAGAAG
  CTAAGGAGCATGACGGACAAGTACCGGCTGCACCTGTCAGTGGCTGACCTCCTCTTTGTCATCACACTCC
  CCTTCTGGGCAGTTGATGCCATGGCTGACTGGTACTTTGGGAAATTTTTGTGTAAGGCTGTCCATATCAT
  CTACACTGTCAACCTCTACAGCAGCGTTCTCATCCTGGCCTTCATCAGCCTGGACCGGTACCTCGCCATT
  GTCCACGCCACCAACAGTCAAAGGCCAAGGAAACTGCTGGCTGAAAAGGCAGTCTATGTGGGCGTCTGGA
  TCCCAGCCCTCCTCCTGACTATACCTGACTTCATCTTTGCCGACGTCAGCCAGGGGGACATCAGTCAGGG
  GGATGACAGGTACATCTGTGACCGCCTTTACCCCGATAGCCTGTGGATGGTGGTGTTTCAATTCCAGCAT
  ATAATGGTGGGTCTCATCCTGCCCGGCATCGTCATCCTCTCCTGTTACTGCATCATCATCTCTAAGCTGT
  CACACTCCAAGGGCCACCAGAAGCGCAAGGCCCTCAAGACGACAGTCATCCTCATCCTAGCTTTCTTTGC
  CTGCTGGCTGCCATATTATGTGGGGATCAGCATCGACTCCTTCATCCTTTTGGGAGTCATCAAGCAAGGA
  TGTGACTTCGAGAGCATTGTGCACAAGTGGATCTCCATCACAGAGGCCCTCGCCTTCTTCCACTGTTGCC
  TGAACCCCATCCTCTATGCCTTCCTCGGGGCCAAGTTCAAAAGCTCTGCCCAGCATGCACTCAACTCCAT
  GAGCAGAGGCTCCAGCCTCAAGATCCTTTCCAAAGGAAAGCGGGGTGGACACTCTTCCGTCTCCACGGAG
  TCAGAATCCTCCAGTTTTCACTCCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205485 representing NM_009911
  Red=Cloning site Green=Tags(s)
  
  MEPISVSIYTSDNYSEEVGSGDYDSNKEPCFRDENVHFNRIFLPTIYFIIFLTGIVGNGLVILVMGYQKK
  LRSMTDKYRLHLSVADLLFVITLPFWAVDAMADWYFGKFLCKAVHIIYTVNLYSSVLILAFISLDRYLAI
  VHATNSQRPRKLLAEKAVYVGVWIPALLLTIPDFIFADVSQGDISQGDDRYICDRLYPDSLWMVVFQFQH
  IMVGLILPGIVILSCYCIIISKLSHSKGHQKRKALKTTVILILAFFACWLPYYVGISIDSFILLGVIKQG
  CDFESIVHKWISITEALAFFHCCLNPILYAFLGAKFKSSAQHALNSMSRGSSLKILSKGKRGGHSSVSTE
  SESSSFHSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009911
• ORF Size: 1077 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009911.2
• RefSeq Size: 1821 bp
• RefSeq ORF: 1080 bp
• Locus ID: 12767
• UniProt ID: P70658
• Cytogenetics: 1 56.43 cM
• Gene Summary: Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation (PubMed:8962122, PubMed:9295051, PubMed:9103415). Involved in the AKT signaling cascade (By similarity). Plays a role in regulation of cell migration, e.g. during wound healing. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes (By similarity). Involved in hematopoiesis and in cardiac ventricular septum formation (PubMed:9634237, PubMed:9634238, PubMed:9689100). Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells (PubMed:9634237). Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival (PubMed:9634238, PubMed:9689100).[UniProtKB/Swiss-Prot Function]