Prr5l (NM_175181) Mouse Tagged ORF Clone
CAT#: MG205410
- TrueORF®
Prr5l (tGFP-tagged) - Mouse RIKEN cDNA 2600010E01 gene (2600010E01Rik)
"NM_175181" in other vectors (1)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Prr5l |
Synonyms | 2600010E01Rik; 4833411O04Rik; Protor-2; Protor2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205410 representing NM_175181
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCCTTCCGCCGGCCTAGACCACGCTTTATGAGCTCCCCTGTGCTCAGCGAGCTGCCCCGCTTCC AGGCTGCCCGGCAGGCTCTGCAGCTAAGTTCCAACTCTGCCTGGAACAGTGTGCAGACAGCTGTGATCAA CGTTTTCAAAGGGGGCGGCTTGCAGAGCAATGAGCTGTATGCACTGAACGAAAGCATCAGGCGGCTGCTG AAGAGTGAACTTGGATCATTCATTACTGACTATTTCCAGAACCAGCTTCTTGCAAAAGGACTGTCCTTTG TGGAGGAGAAGATCAAGCTGTGTGAAGGTGACAATCGGATTGAGGTTCTGGCTGAAGTCTGGGACCACTT CTTCACCGAGACTCTCCCTACCCTGCAGGCAATATTTTATCCGGTTCAGGGACAGGAGCTGACCATCCGC CAGATCTCCCTGCTGGGCTTCCGGGACCTGGTCTTGCTGAAGGTGAAGCTGGGTGACGTGCTGCTGTTGG CACAGTCCAAGTTGCCTTCTTCTGTTATACAAATGCTGCTCATTCTGCAGAGTGTTCACGAGCCCACAGG CCCAAGTGAGGGCTACTTGCAGCTGGAGGAGCTGGTGAAGCAAGTGGTGTCTCCCTTTCTCAGCATCAGC GGGGACCGCAGCTGCTCAGGCCCCACGTACTCACTGGCCAGGAGGCATTCCAGGGTCCGGCCCAAGGTGA CTGTCCTGAACTATGCCTCTCTGATGACCACGGTTGGCCGGCCACTGAACGAGATGGTCTTGACCCCTCT AACAGAACAGGAAGGGGAGGCATACCTGGAGAAGTGTGGCAGTGTCCGGCGGCACACAGTGGCCAACGCC CACTCAGACATCCAGCTGCTGGCGATGGCCACCATGATGCACTCAGGGTTGGGGGAGGAGGCTGGCGGCG AGGACAAACACCTGCTCTTGCCACCCAGCTTCCCCCCACCACACCGCCAGTGCTCCAGTGAACCCAGCAT CCTCGACAGCCCCGATGAACTGGAACTAGAAGACGTGGCAAGTGGCAGCCAGGAGGACTCAGAGCTGAAC TGTGCTTCCCTCAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205410 representing NM_175181
Red=Cloning site Green=Tags(s) MGSFRRPRPRFMSSPVLSELPRFQAARQALQLSSNSAWNSVQTAVINVFKGGGLQSNELYALNESIRRLL KSELGSFITDYFQNQLLAKGLSFVEEKIKLCEGDNRIEVLAEVWDHFFTETLPTLQAIFYPVQGQELTIR QISLLGFRDLVLLKVKLGDVLLLAQSKLPSSVIQMLLILQSVHEPTGPSEGYLQLEELVKQVVSPFLSIS GDRSCSGPTYSLARRHSRVRPKVTVLNYASLMTTVGRPLNEMVLTPLTEQEGEAYLEKCGSVRRHTVANA HSDIQLLAMATMMHSGLGEEAGGEDKHLLLPPSFPPPHRQCSSEPSILDSPDELELEDVASGSQEDSELN CASLS TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_175181 |
ORF Size | 4242 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175181.3 |
RefSeq Size | 2207 bp |
RefSeq ORF | 1113 bp |
Locus ID | 72446 |
UniProt ID | A2AVJ5 |
Cytogenetics | 2 E2 |
Gene Summary | Associates with the mTORC2 complex that regulates cellular processes including survival and organization of the cytoskeleton (By similarity). Regulates the activity of the mTORC2 complex in a substrate-specific manner preventing for instance the specific phosphorylation of PKCs and thereby controlling cell migration (PubMed:22609986). Plays a role in the stimulation of ZFP36-mediated mRNA decay of several ZFP36-associated mRNAs, such as TNF-alpha and GM-CSF, in response to stress. Required for ZFP36 localization to cytoplasmic stress granule (SG) and P-body (PB) in response to stress.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204539 | Prr5l (untagged) - Mouse proline rich 5 like (Prr5l), transcript variant 2, (10ug) |
USD 457.00 |
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