Ahsa1 (NM_146036) Mouse Tagged ORF Clone

CAT#: MG205046

Ahsa1 (tGFP-tagged) - Mouse AHA1, activator of heat shock protein ATPase homolog 1 (yeast) (Ahsa1)

Product Images

Circular map for MG205046

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ahsa1
• Synonyms: BC023857; p38
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205046 representing NM_146036
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGTGGGGTGAGGGAGACCCACGCTGGATCGTGGAGGAGCGGGCGGACGCCACCAACGTCAACA
  ACTGGCACTGGACAGAGAGAGATGCTTCAAATTGGTCCACAGAGAAACTGAAAACCCTGTTCCTGGCCGT
  TCGAGTAGAAAATGAAGAGGGCAAGTGCGAGGTGACCGAAGTGAACAAGCTTGATGGGGAGGCATCCATC
  AACAACAGGAAAGGCAAACTTATCTTCTTCTATGAGTGGACCATCAAACTGAACTGGACAGGTACCTCTA
  AGTCAGGAGTGCAGTACAAAGGACATGTGGAAATCCCCAATTTGTCTGATGAAAATAGCGTGGATGAAGT
  GGAGATTAGTGTGAGCCTTGCCAAAGATGAGCCTGACACAAATCTCGTGGCCTTAATGAAGGAAGACGGG
  GTGAAACTTCTGAGAGAAGCAGTGGGAATTTACATCAGCACCCTCAAGACAGAGTTTACTCAGGGCATGA
  TCTTGCCCACAGTGAATGGAGAGTCAGTAGATCCAGTGGGCCAGCCAGCACTAAAGACTGAGACTTGCAA
  GGCTAAGTCTGCTCCTTCAAAAAGCCAGGCCAAACCTGTTGGTGTCAAAATCCCCACTTGTAAGATCACC
  CTTAAAGAAACCTTCCTGACCTCCCCAGAGGAGCTCTATAGAGTGTTTACCACGCAGGAGCTGGTCCAGG
  CCTTTACCCATGCCCCCGCTGCCTTGGAAGCCGACAGAGGTGGCAAGTTTCACATGGTCGATGGCAACGT
  CACCGGGGAGTTTACTGACCTGGTCCCCGAGAAACACATTGCTATGAAGTGGAGGTTTAAGTCATGGCCA
  GAGGGGCACTTTGCCACCATCACCTTGACCTTCATTGACAAGAATGGAGAGACAGAGCTGTGCATGGAAG
  GCCGTGGCATCCCTGCTCCTGAGGAAGAGCGGACGCGGCAAGGCTGGCAGCGGTACTACTTTGAGGGCAT
  CAAACAGACCTTTGGCTATGGTGCACGCTTGTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205046 representing NM_146036
  Red=Cloning site Green=Tags(s)
  
  MAKWGEGDPRWIVEERADATNVNNWHWTERDASNWSTEKLKTLFLAVRVENEEGKCEVTEVNKLDGEASI
  NNRKGKLIFFYEWTIKLNWTGTSKSGVQYKGHVEIPNLSDENSVDEVEISVSLAKDEPDTNLVALMKEDG
  VKLLREAVGIYISTLKTEFTQGMILPTVNGESVDPVGQPALKTETCKAKSAPSKSQAKPVGVKIPTCKIT
  LKETFLTSPEELYRVFTTQELVQAFTHAPAALEADRGGKFHMVDGNVTGEFTDLVPEKHIAMKWRFKSWP
  EGHFATITLTFIDKNGETELCMEGRGIPAPEEERTRQGWQRYYFEGIKQTFGYGARLF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146036
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146036.2
• RefSeq Size: 1330 bp
• RefSeq ORF: 1017 bp
• Locus ID: 217737
• UniProt ID: Q8BK64
• Cytogenetics: 12 D2
• Gene Summary: Acts as a co-chaperone of HSP90AA1 (PubMed:29127155). Activates the ATPase activity of HSP90AA1 leading to increase in its chaperone activity (PubMed:29127155). Competes with the inhibitory co-chaperone FNIP1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (By similarity). Competes with the inhibitory co-chaperone TSC1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:29127155).[UniProtKB/Swiss-Prot Function]