Il1rl1 (NM_010743) Mouse Tagged ORF Clone

CAT#: MG205016

Il1rl1 (tGFP-tagged) - Mouse interleukin 1 receptor-like 1 (Il1rl1), transcript variant 2

Product Images

Circular map for MG205016

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Il1rl1
• Synonyms: DER4; Fit-1; Ly84; St2; St2-rs1; ST2L; T1; T1/ST2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205016 representing NM_010743
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGACAGACAGAGAATGGGACTTTGGGCTTTGGCAATTCTGACACTTCCCATGTATTTGACAGTTA
  CGGAGGGCAGTAAATCGTCCTGGGGTCTGGAAAATGAGGCTTTAATTGTGAGATGCCCCCAAAGAGGACG
  CTCGACTTATCCTGTGGAATGGTATTACTCAGATACAAATGAAAGTATTCCTACTCAAAAAAGAAATCGG
  ATCTTTGTCTCAAGAGATCGTCTGAAGTTTCTACCAGCCAGAGTGGAAGACTCTGGGATTTATGCTTGTG
  TTATCAGAAGCCCCAACTTGAATAAGACTGGATACTTGAATGTCACCATACATAAAAAGCCGCCAAGCTG
  CAATATCCCTGATTATTTGATGTACTCGACAGTACGTGGATCAGATAAAAATTTCAAGATAACGTGTCCA
  ACAATTGACCTGTATAATTGGACAGCACCTGTTCAGTGGTTTAAGAACTGCAAAGCTCTCCAAGAGCCAA
  GGTTCAGGGCACACAGGTCCTACTTGTTCATTGACAACGTGACTCATGATGATGAAGGTGACTACACTTG
  TCAATTCACACACGCGGAGAATGGAACCAACTACATCGTGACGGCCACCAGATCATTCACAGTTGAAGAA
  AAAGGCTTTTCTATGTTTCCAGTAATTACAAATCCTCCATACAACCACACAATGGAAGTGGAAATAGGAA
  AACCAGCAAGTATTGCCTGTTCAGCTTGCTTTGGCAAAGGCTCTCACTTCTTGGCTGATGTCCTGTGGCA
  GATTAACAAAACAGTAGTTGGAAATTTTGGTGAAGCAAGAATTCAAGAAGAGGAAGGTCGAAATGAAAGT
  TCCAGCAATGACATGGATTGTTTAACCTCAGTGTTAAGGATAACTGGTGTGACAGAAAAGGACCTGTCCC
  TGGAATATGACTGTCTGGCCCTGAACCTTCATGGCATGATAAGGCACACCATAAGGCTGAGAAGGAAACA
  ACCAAGTAAGGAGTGTCCCTCACACATTGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205016 representing NM_010743
  Red=Cloning site Green=Tags(s)
  
  MIDRQRMGLWALAILTLPMYLTVTEGSKSSWGLENEALIVRCPQRGRSTYPVEWYYSDTNESIPTQKRNR
  IFVSRDRLKFLPARVEDSGIYACVIRSPNLNKTGYLNVTIHKKPPSCNIPDYLMYSTVRGSDKNFKITCP
  TIDLYNWTAPVQWFKNCKALQEPRFRAHRSYLFIDNVTHDDEGDYTCQFTHAENGTNYIVTATRSFTVEE
  KGFSMFPVITNPPYNHTMEVEIGKPASIACSACFGKGSHFLADVLWQINKTVVGNFGEARIQEEEGRNES
  SSNDMDCLTSVLRITGVTEKDLSLEYDCLALNLHGMIRHTIRLRRKQPSKECPSHIA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010743
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010743.3
• RefSeq Size: 2667 bp
• RefSeq ORF: 1014 bp
• Locus ID: 17082
• UniProt ID: P14719
• Cytogenetics: 1 19.19 cM
• Gene Summary: Receptor for interleukin-33 (IL-33); signaling requires association of the coreceptor IL1RAP (PubMed:18450470, PubMed:17675517). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8 (By similarity). Possibly involved in helper T-cell function.[UniProtKB/Swiss-Prot Function]