Aptx (BC021872) Mouse Tagged ORF Clone

CAT#: MG204974

Aptx (tGFP-tagged) - Mouse aprataxin (cDNA clone MGC:28249 IMAGE:3994375)

Product Images

Circular map for MG204974

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Aptx
• Synonyms: 2410016G21Rik; AA388047; FHA-HIT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204974 representing BC021872
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAGTGTGCTGGTTGGTGAGACAGGACAGCAGGCACCAGCGAATCAAACTTCCCCATTTGGAAGCTG
  TTGTGATTGGTCGAAGCCCAGAGACCAAGATCACAGATAAGAAATGTTCCCGACAGCAAGTACAGTTGAA
  AGCAGAGTGTAACAAGGGATATGTCAAAGTACAGCAGATGGGGGTCAACCCCACCAGCATTGACTCGGGC
  GTCATCGGGAAGGACCAAGAGAAGCTGCTGCTGCCTGGTCAGGTTCTCCACATGGTGAATGGACTTTATC
  CATACATCGTAGAGTTTGAGGAAGTGGCAGAGAGCCCTAACCTAACACAGAGGAAGAGAAAGAGGTCAGA
  CTGTGATAGTGAGGAGATGGAAGCTGAGTCTGGGACAGGGCTGGCACCTGGGAGCAGCCCCAGCCAGTGC
  TCTGTGTCCCCTAAGAAGGACAAGAATGGAGCCACCAAAAAGGAATCACTGGGCCACTGGAGTCAAGGCT
  TGAAGATGTCTATGAAAGACCCCAAAATGCAGGTTTACAAAGACGACCAGGTGGTGGTGATTAAGGATAA
  ATACCCCAAGGCCCGTCACCACTGGCTGGTCTTACCGTGGGCCTCCATTTCCAGTCTGAAGGTTGTGACC
  AGTGAACACCTTGAACTTCTCAAACATATGCACGCTGTGGGGGAGAAGGTGATAGCAGAGTTTGCTGGAT
  CCAGCAAACTGCGCTTCCGATTGGGCTACCATGCCATTCCCAGCATGAGCCACGTACATCTTCATGTGAT
  CAGCCAGGATTTTGATTCTCCTTGCCTTAAAAACAAAAAGCATTGGAATTCTTTTAATACAGAATACTTT
  CTGGAATCACAAGCTGTGATCAAGATGGTTCAGGAAGCCGGCAGAGTGACTGTTAAAGATGGCACTTGTG
  AGCTCTTGAAGCTGCCTCTCCGTTGCCATGAGTGTCAGCAGCTGCTGCCTTCCATCCCGCAGCTGAAAGA
  GCACCTCAGGAAGCACTGGGGCGGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204974 representing BC021872
  Red=Cloning site Green=Tags(s)
  
  MRVCWLVRQDSRHQRIKLPHLEAVVIGRSPETKITDKKCSRQQVQLKAECNKGYVKVQQMGVNPTSIDSG
  VIGKDQEKLLLPGQVLHMVNGLYPYIVEFEEVAESPNLTQRKRKRSDCDSEEMEAESGTGLAPGSSPSQC
  SVSPKKDKNGATKKESLGHWSQGLKMSMKDPKMQVYKDDQVVVIKDKYPKARHHWLVLPWASISSLKVVT
  SEHLELLKHMHAVGEKVIAEFAGSSKLRFRLGYHAIPSMSHVHLHVISQDFDSPCLKNKKHWNSFNTEYF
  LESQAVIKMVQEAGRVTVKDGTCELLKLPLRCHECQQLLPSIPQLKEHLRKHWGG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC021872
• ORF Size: 1007 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021872, AAH21872
• RefSeq Size: 1407 bp
• RefSeq ORF: 1007 bp
• Locus ID: 66408
• Cytogenetics: 4 A5
• Gene Summary: DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined (PubMed:16964241). Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity (By similarity). Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA) (By similarity).[UniProtKB/Swiss-Prot Function]