Fam49b (NM_144846) Mouse Tagged ORF Clone

CAT#: MG204712

Fam49b (tGFP-tagged) - Mouse RIKEN cDNA 0910001A06 gene (0910001A06Rik)

Product Images

Circular map for MG204712

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fam49b
• Synonyms: 0910001A06Rik; AW122079
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204712 representing NM_144846
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAATCTTCTTAAAGTTTTGACATGCACAGACCTTGAGCAGGGGCCAAATTTTTTCCTTGATTTTG
  AAAATGCCCAGCCTACAGAGTCTGAGAAGGAAATATATAATCAGGTGAATGTAGTGTTAAAAGATGCAGA
  AGGCATCTTGGAGGATTTGCAGTCATACAGAGGAGCTGGCCACGAAATACGAGAGGCAATCCAGCATCCA
  GCCGATGAGAAGTTGCAAGAGAAGGCGTGGGGTGCAGTCGTGCCACTAGTAGGCAAATTAAAGAAGTTTT
  ACGAATTTTCTCAGAGACTAGAAGCAGCACTAAGAGGCCTTCTGGGAGCCTTGACTAGTACGCCATACTC
  GCCCACCCAACATCTAGAGCGAGAGCAGGCTCTCGCTAAACAGTTTGCAGAGATTCTTCACTTCACACTC
  CGGTTTGATGAGCTCAAGATGACAAATCCTGCCATACAAAATGACTTCAGCTACTACAGAAGAACATTGA
  GTCGTATGAGAATTAATAATGTCCCGGCAGAAGGAGAAAATGAAGTAAATAATGAATTGGCAAATCGAAT
  GTCATTGTTTTACGCTGAGGCCACCCCAATGCTGAAAACCTTAAGTGATGCAACAACAAAATTTGTATCG
  GAGAATAAAAATTTGCCAATAGAAAATACCACAGATTGTTTAAGCACCATGGCAAGTGTATGCAGAGTCA
  TGCTGGAGACACCGGAATACAGAAGCAGATTTACCAATGAAGAGACAGTATCATTCTGCTTGAGGGTAAT
  GGTGGGTGTCATAATACTCTATGACCACGTCCATCCAGTGGGAGCATTTGCCAAAACTTCTAAGATTGAT
  ATGAAAGGTTGTATCAAAGTTCTTAAGGACCAACCTCCTAATAGTGTAGAAGGTCTTCTCAATGCTCTCA
  GGTACACAACAAAACATTTGAATGATGAGACTACCTCCAAGCAAATTAGGTCCATGCTGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204712 representing NM_144846
  Red=Cloning site Green=Tags(s)
  
  MGNLLKVLTCTDLEQGPNFFLDFENAQPTESEKEIYNQVNVVLKDAEGILEDLQSYRGAGHEIREAIQHP
  ADEKLQEKAWGAVVPLVGKLKKFYEFSQRLEAALRGLLGALTSTPYSPTQHLEREQALAKQFAEILHFTL
  RFDELKMTNPAIQNDFSYYRRTLSRMRINNVPAEGENEVNNELANRMSLFYAEATPMLKTLSDATTKFVS
  ENKNLPIENTTDCLSTMASVCRVMLETPEYRSRFTNEETVSFCLRVMVGVIILYDHVHPVGAFAKTSKID
  MKGCIKVLKDQPPNSVEGLLNALRYTTKHLNDETTSKQIRSMLQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144846
• ORF Size: 972 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144846.5
• RefSeq Size: 3798 bp
• RefSeq ORF: 975 bp
• Locus ID: 223601
• UniProt ID: Q921M7
• Cytogenetics: 15 D1
• Gene Summary: Negatively regulates RAC1 signaling and RAC1-driven cytoskeletal remodeling (PubMed:31285585). Regulates chemotaxis, cell migration and epithelial polarization by controlling the polarity, plasticity, duration and extent of protrusions. Limits Rac1 mediated activation of the Scar/WAVE complex, focuses protrusion signals and regulates pseudopod complexity by inhibiting Scar/WAVE-induced actin polymerization (By similarity). Protects against Salmonella bacterial infection. Attenuates processes such as macropinocytosis, phagocytosis and cell migration and restrict sopE-mediated bacterial entry (PubMed:31285585). Restricts also infection mediated by Mycobacterium tuberculosis and Listeria monocytogenes (PubMed:31285585). Involved in the regulation of mitochondrial dynamics and oxidative stress (PubMed:29059164).[UniProtKB/Swiss-Prot Function]