Fam49b (NM_144846) Mouse Untagged Clone

CAT#: MC201015

Fam49b (untagged) - Mouse family with sequence similarity 49, member B (Fam49b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fam49b
• Synonyms: 0910001A06Rik; AW122079
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC011343 sequence for NM_144846
  GGCTCCCCCTAGCGCCGTGACGAGCCGCCGGCGGCCGAGAGAAGCGCGGCCGAGTGCCCGAGCTCGCGGC GGATAGAAGGACAAGGACAGAATCACCAGCACTGGCTGAAGGTACCTTAACATGGGGAATCTTCTTAAAG TTTTGACATGCACAGACCTTGAGCAGGGGCCAAATTTTTTCCTTGATTTTGAAAATGCCCAGCCTACAGA GTCTGAGAAGGAAATATATAATCAGGTGAATGTAGTGTTAAAAGATGCAGAAGGCATCTTGGAGGATTTG CAGTCATACAGAGGAGCTGGCCACGAAATACGAGAGGCAATCCAGCATCCAGCCGATGAGAAGTTGCAAG AGAAGGCGTGGGGTGCAGTCGTGCCACTAGTAGGCAAATTAAAGAAGTTTTACGAATTTTCTCAGAGACT AGAAGCAGCACTAAGAGGCCTTCTGGGAGCCTTGACTAGTACGCCATACTCGCCCACCCAACATCTAGAG CGAGAGCAGGCTCTCGCTAAACAGTTTGCAGAGATTCTTCACTTCACACTCCGGTTTGATGAGCTCAAGA TGACAAATCCTGCCATACAAAATGACTTCAGCTACTACAGAAGAACATTGAGTCGTATGAGAATTAATAA TGTCCCGGCAGAAGGAGAAAATGAAGTAAATAATGAATTGGCAAATCGAATGTCATTGTTTTACGCTGAG GCCACCCCAATGCTGAAAACCTTAAGTGATGCAACAACAAAATTTGTATCGGAGAATAAAAATTTGCCAA TAGAAAATACCACAGATTGTTTAAGCACCATGGCAAGTGTATGCAGAGTCATGCTGGAGACACCGGAATA CAGAAGCAGATTTACCAATGAAGAGACAGTATCATTCTGCTTGAGGGTAATGGTGGGTGTCATAATACTC TATGACCACGTCCATCCAGTGGGAGCATTTGCCAAAACTTCTAAGATTGATATGAAAGGTTGTATCAAAG TTCTTAAGGACCAACCTCCTAATAGTGTAGAAGGTCTTCTCAATGCTCTCAGGTACACAACAAAACATTT GAATGATGAGACTACCTCCAAGCAAATTAGGTCCATGCTGCAGTAAGAGCTGTGGAGCAAGCCCCTACTG CAGATGGAGCACACAGTGTTCTTCAGTGACCGAGAATGCACAGTCTTAGTGAATGCCATCAAATCTCCTT ATTCTTGCTTTGGTTTCTTTCCTCTGTTCCTCTTCCCACTTTTTAAATCATGTTCTTGTTCTTAAGACTT CTTTTCTGTGCCAAAATCAGTAAAGTTATACTTTGAAAGGGATTTTGTCCTTTCAAAACAGGCCATCTAA GGCAGCTAATTACGCATTGCATTGAGGTCTCTACTGAGAAAAGTTCTGTGACTTGAACTAAATATTTTTA AATGTGGATTTTTTTTGAAAACTAATATTTAATATTGCTTCTCCTGCATGGCGAAACTGCCTATTCTGCT ATTTAAAACCCTCATAAATTTATTTTCTACTGCCGCTTTTTCATGTGCAGCCAAAATGGAAATGTTTAAA TTAACTGTGTTGTACAAATGGTACCCAACACAAAACCTTTTTTAAATTAGTAAGACTTTGTTTAAAGTTT TAAGTTTGCAGGTTGACTTTTTTTTAAGGTTGTATGTTGTGTGTTTAACATTTATTAACTAACGTTAAAA ACTGTGATGTGTGCGTAGAATATTACGTATGCATGTTCATGTTTAAGAATGGCTGTTGATAAAATAAAAA TCAGCTTTCCTTTTTCTAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_144846
• Insert Size: 975 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC011343, AAH11343
• RefSeq Size: 1786 bp
• RefSeq ORF: 975 bp
• Locus ID: 223601
• UniProt ID: Q921M7
• Cytogenetics: 15 D1
• Gene Summary: Negatively regulates RAC1 signaling and RAC1-driven cytoskeletal remodeling (PubMed:31285585). Regulates chemotaxis, cell migration and epithelial polarization by controlling the polarity, plasticity, duration and extent of protrusions. Limits Rac1 mediated activation of the Scar/WAVE complex, focuses protrusion signals and regulates pseudopod complexity by inhibiting Scar/WAVE-induced actin polymerization (By similarity). Protects against Salmonella bacterial infection. Attenuates processes such as macropinocytosis, phagocytosis and cell migration and restrict sopE-mediated bacterial entry (PubMed:31285585). Restricts also infection mediated by Mycobacterium tuberculosis and Listeria monocytogenes (PubMed:31285585). Involved in the regulation of mitochondrial dynamics and oxidative stress (PubMed:29059164).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1. 2, 3, and 4 encode the same protein.