Apex1 (NM_009687) Mouse Tagged ORF Clone

CAT#: MG204563

Apex1 (tGFP-tagged) - Mouse apurinic/apyrimidinic endonuclease 1 (Apex1)

Product Images

Circular map for MG204563

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Apex1
• Synonyms: APE; Apex; HAP1; Ref-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204563 representing NM_009687
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAAAGCGGGGAAAGAAAGCGGCGGCCGACGACGGGGAAGAACCCAAGTCGGAGCCAGAGACCAAGA
  AGAGTAAGGGGGCAGCAAAGAAAACCGAGAAGGAGGCCGCGGGAGAGGGCCCTGTCCTGTACGAGGACCC
  TCCAGATCAGAAAACCTCACCCAGTGGCAAATCTGCCACACTCAAGATATGCTCCTGGAATGTGGATGGG
  CTTCGAGCCTGGATTAAAAAGAAAGGTTTGGATTGGGTAAAGGAAGAAGCACCAGATATCTTGTGCCTCC
  AAGAGACCAAGTGCTCGGAGAACAAACTCCCGGCTGAACTGCAAGAGCTGCCTGGACTCACCCATCAGTA
  CTGGTCAGCTCCGTCAGACAAAGAAGGATACAGTGGTGTGGGCCTACTTTCCCGCCAGTGCCCGCTAAAA
  GTCTCTTATGGCATTGGCGAGGAAGAACATGATCAAGAAGGCCGGGTGATTGTGGCTGAATTTGAGTCCT
  TTGTCCTGGTAACAGCCTATGTTCCCAATGCAGGCAGGGGTCTGGTAAGACTGGAATACCGACAGCGTTG
  GGATGAAGCCTTCCGAAAGTTTCTAAAGGACTTGGCTTCCAGAAAGCCTCTTGTGCTATGTGGGGATCTC
  AATGTGGCTCATGAAGAAATTGACCTCCGTAACCCCAAAGGAAACAAAAAGAATGCTGGCTTTACTCCCC
  AGGAGCGCCAAGGTTTTGGGGAACTGCTACAAGCTGTACCATTGGCTGACAGCTTCCGGCATCTCTACCC
  CAACACTGCTTACGCTTACACTTTCTGGACTTACATGATGAATGCCCGCTCTAAGAATGTTGGTTGGCGC
  CTTGATTACTTTTTGCTTTCCCACTCTCTTTTACCTGCATTGTGTGACAGCAAGATCCGGTCCAAGGCTC
  TTGGCAGTGACCACTGTCCCATCACCCTTTACCTAGCACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204563 representing NM_009687
  Red=Cloning site Green=Tags(s)
  
  MPKRGKKAAADDGEEPKSEPETKKSKGAAKKTEKEAAGEGPVLYEDPPDQKTSPSGKSATLKICSWNVDG
  LRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPAELQELPGLTHQYWSAPSDKEGYSGVGLLSRQCPLK
  VSYGIGEEEHDQEGRVIVAEFESFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKDLASRKPLVLCGDL
  NVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTAYAYTFWTYMMNARSKNVGWR
  LDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009687
• ORF Size: 951 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009687.2
• RefSeq Size: 1185 bp
• RefSeq ORF: 954 bp
• Locus ID: 11792
• UniProt ID: P28352
• Cytogenetics: 14 26.3 cM
• Gene Summary: Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA.[UniProtKB/Swiss-Prot Function]