Lgals8 (NM_018886) Mouse Tagged ORF Clone

CAT#: MG204541

Lgals8 (tGFP-tagged) - Mouse lectin, galactose binding, soluble 8 (Lgals8)

Product Images

Circular map for MG204541

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lgals8
• Synonyms: 1200015E08Rik; AI326142; D13Ertd524e; Lgals-8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204541 representing NM_018886
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTCCTTAAATAACCTACAAAATATCATCTATAACCCGATAATCCCCTATGTTGGCACCATTACTG
  AGCAATTGAAGCCTGGCTCTCTGATTGTAATCCGTGGGCATGTCCCTAAAGATTCAGAAAGATTCCAGGT
  TGACTTTCAGCTGGGCAACAGCCTGAAGCCAAGAGCAGACGTGGCCTTCCACTTTAACCCTCGGTTCAAA
  AGGTCTAGCTGCATTGTTTGTAACACACTGACACAGGAGAAGTGGGGCTGGGAGGAGATCACCTACGACA
  TGCCCTTCAGAAAAGAAAAGTCCTTTGAGATCGTGTTCATGGTGCTCAAGAACAAATTCCAGGTGGCTGT
  GAACGGAAGGCATGTTCTGCTGTACGCCCACAGGATCAGCCCGGAGCAGATCGACACAGTGGGCATCTAC
  GGCAAAGTGAACATCCACTCCATCGGGTTCAGATTCAGCTCGGATTTACAGAGTATGGAAACATCTGCTC
  TGGGACTGACACAGATAAACAGAGAGAATATACAAAAGCCAGGCAAGCTCCAGCTGAGCCTGCCATTTGA
  AGCAAGGTTGAATGCCTCCATGGGTCCTGGACGAACCGTTGTCATTAAAGGGGAAGTGAACACCAATGCC
  CGAAGCTTTAATGTTGACCTAGTGGCAGGAAAAACAAGGGATATCGCTCTGCACTTGAACCCACGCCTCA
  ATGTGAAAGCATTTGTAAGAAATTCCTTTCTTCAGGATGCCTGGGGAGAAGAGGAGAGAAATATTACCTG
  CTTCCCATTTAGTTCTGGGATGTACTTTGAGATGATAATCTACTGTGATGTCCGGGAATTCAAGGTTGCT
  ATAAATGGTGTGCACAGCCTGGAGTACAAACACAGATTTAAAGACCTAAGCAGTATTGATACACTATCAG
  TCGATGGTGATATCCGTTTGCTGGATGTAAGGAGCTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204541 representing NM_018886
  Red=Cloning site Green=Tags(s)
  
  MLSLNNLQNIIYNPIIPYVGTITEQLKPGSLIVIRGHVPKDSERFQVDFQLGNSLKPRADVAFHFNPRFK
  RSSCIVCNTLTQEKWGWEEITYDMPFRKEKSFEIVFMVLKNKFQVAVNGRHVLLYAHRISPEQIDTVGIY
  GKVNIHSIGFRFSSDLQSMETSALGLTQINRENIQKPGKLQLSLPFEARLNASMGPGRTVVIKGEVNTNA
  RSFNVDLVAGKTRDIALHLNPRLNVKAFVRNSFLQDAWGEEERNITCFPFSSGMYFEMIIYCDVREFKVA
  INGVHSLEYKHRFKDLSSIDTLSVDGDIRLLDVRSW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018886
• ORF Size: 948 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018886.5, NP_061374.1
• RefSeq Size: 2810 bp
• RefSeq ORF: 951 bp
• Locus ID: 56048
• UniProt ID: Q9JL15
• Cytogenetics: 13 4.64 cM
• Gene Summary: Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy. Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture. Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52. Required to restrict infection of bacterial invasion such as S.typhimurium. Also required to restrict infection of Picornaviridae viruses. Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.[UniProtKB/Swiss-Prot Function]