Ier5 (NM_010500) Mouse Tagged ORF Clone

CAT#: MG204347

Ier5 (tGFP-tagged) - Mouse immediate early response 5 (Ier5)

Product Images

Circular map for MG204347

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ier5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204347 representing NM_010500
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTCAAGCTGGAGGCTCACCGCATCGTCAGCATCTCCCTGGGCAAGATCTACAACTCGCGGGTCC
  AGCGCGGCGGCATCAAGCTGCACAAGAACCTCTTGGTGTCGTTGGTGCTGCGCAGCGCCCGCCAAGTCTA
  CCTGAGTGACCCCTGCCCTGGCCTCTACCTGGCTGGCCCTGCGGGGACCCCGGCGGTGCCGCCGCCTCAG
  CAGCCTGGAGAGCCTGTAGCCGGGCCACCCTCCGGCTGGGGAGAGCCGCCCCCGCCGGTCGCCCGTGCCG
  CCTGGCCGGAGCCCGAGCCTCAGCCGCAGCCTCAGCCTCAGCGTCCCTCCGTGTGCCACACGCCCGGAGC
  GGGAAGCTCAGAACCGGTGGCCGCGGTGGCCGGATCGGGGGAAGCTCTCCGGGGCGGAGAGGAGGACTCG
  GCGGCAGCTGCTTGGGGCCGCGTGGAGCGTCCGCGCGCGGCGTCTTCAGGAGGTGGCTCGGACGCGTGCC
  CCGAGGGGCCCCGGGCAGTGCGCCGCCCGTGCGGCTGTCCCCCGGCCGTGGAGGAGCGGTCCTCCGAGGA
  CGGGTCCCCCGCGCCACCCGCCCCGTGTCCCAGGAAGCGTGGCGCAGCGGGAGTGGGTGGCGGCCGCGCG
  AGCTGCTCGGCACCCGGCTCTACCCCTCTCAAGAAGCCGCGCCGGAACTCGGAGGAGCAGCCAGTCACCG
  GCGAGGACGACACCGACGAGGAGATGGAGACCGGGAACGTGGCAAACCTCATCAGCATCTTCGGGTCTAG
  CTTCTCGGGACTCCTACGGAAAAGCCCCGCAGGCGGCCGAGAGGAAGAGGAGGCGGAGGAGGGCGGCCCG
  GAAGCCGCCGAGCCGGGGCAGATCTGCTGCGACAAGCCGGTGCTGAGAGACATGAGCCCCTGGAGCACAG
  CCATCGTGGCCTTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204347 representing NM_010500
  Red=Cloning site Green=Tags(s)
  
  MEFKLEAHRIVSISLGKIYNSRVQRGGIKLHKNLLVSLVLRSARQVYLSDPCPGLYLAGPAGTPAVPPPQ
  QPGEPVAGPPSGWGEPPPPVARAAWPEPEPQPQPQPQRPSVCHTPGAGSSEPVAAVAGSGEALRGGEEDS
  AAAAWGRVERPRAASSGGGSDACPEGPRAVRRPCGCPPAVEERSSEDGSPAPPAPCPRKRGAAGVGGGRA
  SCSAPGSTPLKKPRRNSEEQPVTGEDDTDEEMETGNVANLISIFGSSFSGLLRKSPAGGREEEEAEEGGP
  EAAEPGQICCDKPVLRDMSPWSTAIVAF
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010500
• ORF Size: 924 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010500.2, NP_034630.1
• RefSeq Size: 2123 bp
• RefSeq ORF: 927 bp
• Locus ID: 15939
• UniProt ID: O89113
• Cytogenetics: 1 66.45 cM
• Gene Summary: Plays a role as a transcription factor. Mediates positive transcriptional regulation of several chaperone gene during the heat shock response in a HSF1-dependent manner. Mediates negative transcriptional regulation of CDC25B expression. Plays a role in the dephosphorylation of the heat shock factor HSF1 and ribosomal protein S6 kinase (S6K) by the protein phosphatase PP2A. Involved in the regulation of cell proliferation and resistance to thermal stress. Involved in the cell cycle checkpoint and survival in response to ionizing radiation. Associates with chromatin to the CDC25B promoter.[UniProtKB/Swiss-Prot Function]