Ier5 (NM_010500) Mouse Untagged Clone

CAT#: MC207308

Ier5 (untagged) - Mouse immediate early response 5 (Ier5), (10ug)


  "NM_010500" in other vectors (4)

Reconstitution Protocol

USD 330.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Ier5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC207308 representing NM_010500
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTTCAAGCTGGAGGCTCACCGCATCGTCAGCATCTCCCTGGGCAAGATCTACAACTCGCGGGTCC
AGCGCGGCGGCATCAAGCTGCACAAGAACCTCTTGGTGTCGTTGGTGCTGCGCAGCGCCCGCCAAGTCTA
CCTGAGTGACCCCTGCCCTGGCCTCTACCTGGCTGGCCCTGCGGGGACCCCGGCGGTGCCGCCGCCTCAG
CAGCCTGGAGAGCCTGTAGCCGGGCCACCCTCCGGCTGGGGAGAGCCGCCCCCGCCGGTCGCCCGTGCCG
CCTGGCCGGAGCCCGAGCCTCAGCCGCAGCCTCAGCCTCAGCGTCCCTCCGTGTGCCACACGCCCGGAGC
GGGAAGCTCAGAACCGGTGGCCGCGGTGGCCGGATCGGGGGAAGCTCTCCGGGGCGGAGAGGAGGACTCG
GCGGCAGCTGCTTGGGGCCGCGTGGAGCGTCCGCGCGCGGCGTCTTCAGGAGGTGGCTCGGACGCGTGCC
CCGAGGGGCCCCGGGCAGTGCGCCGCCCGTGCGGCTGTCCCCCGGCCGTGGAGGAGCGGTCCTCCGAGGA
CGGGTCCCCCGCGCCACCCGCCCCGTGTCCCAGGAAGCGTGGCGCAGCGGGAGTGGGTGGCGGCCGCGCG
AGCTGCTCGGCACCCGGCTCTACCCCTCTCAAGAAGCCGCGCCGGAACTCGGAGGAGCAGCCAGTCACCG
GCGAGGACGACACCGACGAGGAGATGGAGACCGGGAACGTGGCAAACCTCATCAGCATCTTCGGGTCTAG
CTTCTCGGGACTCCTACGGAAAAGCCCCGCAGGCGGCCGAGAGGAAGAGGAGGCGGAGGAGGGCGGCCCG
GAAGCCGCCGAGCCGGGGCAGATCTGCTGCGACAAGCCGGTGCTGAGAGACATGAGCCCCTGGAGCACAG
CCATCGTGGCCTTCTGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_010500
Insert Size 927 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_010500.2, NP_034630.1
RefSeq Size 3272 bp
RefSeq ORF 927 bp
Locus ID 15939
UniProt ID O89113
Cytogenetics 1 66.45 cM
Gene Summary Plays a role as a transcription factor. Mediates positive transcriptional regulation of several chaperone gene during the heat shock response in a HSF1-dependent manner. Mediates negative transcriptional regulation of CDC25B expression. Plays a role in the dephosphorylation of the heat shock factor HSF1 and ribosomal protein S6 kinase (S6K) by the protein phosphatase PP2A. Involved in the regulation of cell proliferation and resistance to thermal stress. Involved in the cell cycle checkpoint and survival in response to ionizing radiation. Associates with chromatin to the CDC25B promoter.[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.