Kctd15 (NM_146188) Mouse Tagged ORF Clone

CAT#: MG203833

Kctd15 (tGFP-tagged) - Mouse potassium channel tetramerisation domain containing 15 (Kctd15)

Product Images

Circular map for MG203833

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kctd15
• Synonyms: BC031749
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203833 representing NM_146188
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCACCGCAAGGAGCGGCCGAGCGGGTCCTCGCTTAACGCACACGGCAGCAGCGGCACGGCGGAGG
  GAGGAAATATGTCCCGGCTGTCACTCACCCGGTCGCCTGTGTCTCCCCTGGCTGCCCAGGGGATCCCACT
  GCCAGCTCAGCTCACCAAAGCCAACGCACCCGTGCACATCGATGTGGGAGGTCACATGTATACGAGCAGC
  CTGGCCACACTCACCAAGTACCCTGACTCCAGAATAAGCCGTCTCTTCAACGGCACGGAGCCCATTGTCC
  TAGACAGTCTCAAGCAACATTATTTCATCGACCGCGATGGGGAGATTTTCCGCTACATACTGAGTTTCCT
  GCGGACGTCAAAACTGCTGCTTCCAGATGACTTCAAGGACTTCAACCTGCTGTATGAGGAGGCAAGGTAC
  TACCAGCTGCAGCCCATGGTGCGTGAGCTGGAGCGCTGGCAGCAAGACCAAGAGCAAAGACGTCGCAGCC
  GGGCCTGCGACTGCCTGGTGGTACGGGTCACCCCTGACTTGGGTGAACGGATCGCACTCAGTGGTGAGAA
  GGCCCTCATTGAGGAGGTCTTCCCGGAGACTGGAGACGTCATGTGCAACTCTGTCAATGCTGGCTGGAAC
  CAGGACCCCACACACGTCATCCGTTTCCCTCTCAACGGCTACTGTAGACTCAACTCAGTGCAGGTCCTAG
  AAAGGCTGTTTCAGAGAGGGTTCAGCGTGGCCGCATCCTGCGGGGGTGGTGTGGACTCTTCCCAGTTCAG
  CGAATATGTTCTTTGCCGGGAAGAGCGGCGACCACAGCCTACCCCAACTGCTGTCCGGATAAAGCAGGAG
  CCCCTGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203833 representing NM_146188
  Red=Cloning site Green=Tags(s)
  
  MPHRKERPSGSSLNAHGSSGTAEGGNMSRLSLTRSPVSPLAAQGIPLPAQLTKANAPVHIDVGGHMYTSS
  LATLTKYPDSRISRLFNGTEPIVLDSLKQHYFIDRDGEIFRYILSFLRTSKLLLPDDFKDFNLLYEEARY
  YQLQPMVRELERWQQDQEQRRRSRACDCLVVRVTPDLGERIALSGEKALIEEVFPETGDVMCNSVNAGWN
  QDPTHVIRFPLNGYCRLNSVQVLERLFQRGFSVAASCGGGVDSSQFSEYVLCREERRPQPTPTAVRIKQE
  PLD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146188
• ORF Size: 849 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146188.2
• RefSeq Size: 2489 bp
• RefSeq ORF: 852 bp
• Locus ID: 233107
• UniProt ID: Q8K0E1
• Cytogenetics: 7 B2
• Gene Summary: During embryonic development, interferes with neural crest formation. Inhibits AP2 transcriptional activity by interaction with its activation domain (By similarity).[UniProtKB/Swiss-Prot Function]