Bpifa1 (NM_011126) Mouse Tagged ORF Clone

CAT#: MG203753

Bpifa1 (tGFP-tagged) - Mouse palate, lung, and nasal epithelium carcinoma associated (Plunc)

Product Images

Circular map for MG203753

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bpifa1
• Synonyms: LUNX; NASG; Plunc; SPLUNC1; SPURT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203753 representing NM_011126
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCTAGTTGGGAGCCTCGTTGTCCTCTGTGGGCTGCTGGCCCACAGCACAGCACAGCTGGCAGGCT
  TGCCATTGCCCCTGGGCCAGGGTCCACCCTTGCCACTGAACCAGGGCCCACCGTTGCCACTGAACCAGGG
  CCAGCTGTTGCCCCTGGCTCAGGGTCTGCCTTTGGCTGTAAGCCCAGCACTGCCTTCAAATCCCACAGAT
  CTTCTTGCTGGAAAATTCACAGATGCTCTCAGCGGTGGCCTGCTGTCTGGGGGGCTGCTGGGCATTTTGG
  AAAATATTCCACTCCTGGATGTTATAAAGTCTGGAGGAGGCAATTCTAATGGCCTTGTTGGGGGCCTGCT
  GGGAAAACTGACGTCATCAGTTCCTCTCCTGAACAACATCCTCGACATAAAAATCACTGATCCGCAGCTG
  CTAGAACTTGGTCTTGTGCAGAGTCCTGATGGCCATCGTCTCTATGTCACCATCCCTCTGGGCTTGACAC
  TCAACGTAAATATGCCCGTAGTTGGAAGTCTTTTGCAATTGGCTGTGAAGCTGAACATTACTGCAGAAGT
  CTTAGCCGTGAAAGACAATCAGGGGAGGATTCATCTGGTTCTTGGTGACTGCACCCACTCCCCTGGCAGC
  CTGAAAATCAGCTTGCTCAATGGAGTCACTCCTGTTCAAAGCTTTTTAGACAACCTCACAGGGATATTGA
  CTAAAGTCCTTCCTGAGCTGATCCAGGGCAAGGTATGTCCTCTGGTCAATGGGATTCTCAGCGGTTTGGA
  TGTCACCCTGGTGCACAACATTGCTGAATTACTGATCCATGGACTACAGTTTGTCATCAAAGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203753 representing NM_011126
  Red=Cloning site Green=Tags(s)
  
  MFLVGSLVVLCGLLAHSTAQLAGLPLPLGQGPPLPLNQGPPLPLNQGQLLPLAQGLPLAVSPALPSNPTD
  LLAGKFTDALSGGLLSGGLLGILENIPLLDVIKSGGGNSNGLVGGLLGKLTSSVPLLNNILDIKITDPQL
  LELGLVQSPDGHRLYVTIPLGLTLNVNMPVVGSLLQLAVKLNITAEVLAVKDNQGRIHLVLGDCTHSPGS
  LKISLLNGVTPVQSFLDNLTGILTKVLPELIQGKVCPLVNGILSGLDVTLVHNIAELLIHGLQFVIKV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011126
• ORF Size: 834 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011126.3
• RefSeq Size: 1113 bp
• RefSeq ORF: 837 bp
• Locus ID: 18843
• UniProt ID: P97361
• Cytogenetics: 2 H1
• Gene Summary: Lipid-binding protein which shows high specificity for the surfactant phospholipid dipalmitoylphosphatidylcholine (DPPC) (By similarity). Plays a role in the innate immune responses of the upper airways (PubMed:23499554). Reduces the surface tension in secretions from airway epithelia and inhibits the formation of biofilm by pathogenic Gram-negative bacteria, such as P.aeruginosa and K.pneumoniae (PubMed:23499554). Negatively regulates proteolytic cleavage of SCNN1G, an event that is required for activation of the epithelial sodium channel (ENaC), and thereby contributes to airway surface liquid homeostasis and proper clearance of mucus (By similarity). Plays a role in the airway inflammatory response after exposure to irritants (By similarity). May attract macrophages and neutrophils (By similarity).[UniProtKB/Swiss-Prot Function]