Emcn (BC003706) Mouse Tagged ORF Clone

CAT#: MG203408

Emcn (tGFP-tagged) - Mouse endomucin (cDNA clone MGC:5673 IMAGE:3485788)

Product Images

Circular map for MG203408

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Emcn
• Synonyms: 0610012K22Rik; AI315669; Muc14
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203408 representing BC003706
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCTGCTTCAAGCGACTGTTCTTTTCTTCCTTTTGTCCAACAGTCTCTGCCACAGTGAGGACGGCA
  AAGATGTTCAGAATGACTCTATTCCAACACCGGCAGAAACTTCCACAACAAAAGCCTCTGTCACAATACC
  AGGCATCGTGTCAGTGACAAATCCTAACAAACCAGCAGATGGAACACCTCCCGAAGGAACGACCAAAAGT
  GACGTATCCCAAACATCTTTGGTGACAACAATTAATTCACTGACAACACCAAAACATGAAGTGGGAACAA
  CAACTGAAGGTCCCCTAAGGAATGAAAGTTCCACTATGAAAATAACAGTGCCAAATACTCCAACTTCAAA
  TGCTAATTCAACGCTACCAGGTTCCCAGAACAAGACTGAGAATCAGAGTTCAATCAGAACAACGGAAATA
  TCAGTTACTACTCAACTACTGGATGCCTTGCCGAAAATCACTGCAACATCCTCTGCATCTCTAACAACTG
  CACACACCATGTCACTGCTTCAAGATACTGAGGATAGGAAGATTGCAACCACTCCATCAACCACCCCCTC
  CTATTCCAGTATCATTTTGCCTGTGGTTATCGCGCTGGTTGTTATAACACTCTTGGTATTTACTCTGGTG
  GGTTTGTATCGAATATGCTGGAAGAGAGACCCAGGCACTCCAGAAAACGGAAATGATCAGCCTCAGTCCG
  ATAAAGAGAGTGTGAAGCTGCTCACTGTTAAGACAATCTCTCATGAGTCTGGCGAACACTCTGCACAGGG
  GAAAACCAAGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203408 representing BC003706
  Red=Cloning site Green=Tags(s)
  
  MRLLQATVLFFLLSNSLCHSEDGKDVQNDSIPTPAETSTTKASVTIPGIVSVTNPNKPADGTPPEGTTKS
  DVSQTSLVTTINSLTTPKHEVGTTTEGPLRNESSTMKITVPNTPTSNANSTLPGSQNKTENQSSIRTTEI
  SVTTQLLDALPKITATSSASLTTAHTMSLLQDTEDRKIATTPSTTPSYSSIILPVVIALVVITLLVFTLV
  GLYRICWKRDPGTPENGNDQPQSDKESVKLLTVKTISHESGEHSAQGKTKN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC003706
• ORF Size: 785 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003706, AAH03706
• RefSeq Size: 1437 bp
• RefSeq ORF: 785 bp
• Locus ID: 59308
• Cytogenetics: 3 G3
• Gene Summary: Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.[UniProtKB/Swiss-Prot Function]