Fam58b (NM_197989) Mouse Tagged ORF Clone

CAT#: MG203196

Fam58b (tGFP-tagged) - Mouse RIKEN cDNA 1810009O10 gene (1810009O10Rik)

Product Images

Circular map for MG203196

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fam58b
• Synonyms: 1810009O10Rik; Ccnq; Fam58a
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203196 representing NM_197989
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCTGTTCGGCCAGACAGCTGCGAAAGCGGGCCGGCGGCGCCGCGAGCCGAGGAGAGGCCGGCTC
  CCGAGGCGAGGGTGCACTTCCGAGTAACCCGGTTTATCATGGAGGCAGGCGTCAAGCTAGGCATGCAGTC
  CATCCCCATCGCCACCGCTTGCACCATTTACCATAAGTTCTTCTGCGAGATCAACCTGGACGCATATGAC
  CTCTACCTGGTCGCCATGTCTTCCATTTACTTGGCCGGCAAAGTGGAGGAGCAACACTTGCGTACTCGTG
  ACATCATCAACGTGTCGCACAGGTACTTTAACCCGGGCAGTGAGCCATTGGAGCTGGATTCCCGCTTCTG
  GGAGCTACGAGACAGCATTGTACAATGTGAGCTTCTTATGCTACGAGTTCTGCGCTTCCAAGTCTCTTTC
  CAGCATCCACACAAGTACCTGCTGCACTACTTGATTTCCCTCAAGAACTGGCTGAATCGTTACAGCTGGC
  AACGAACCCCCATCTCAGTTACAGCTTGGGCTCTGCTACGAGATAGCTACCACGGGGGGCTATGCCTCCG
  CTTCCAGGCTCAGCACCTAGCCGTGGCTGTGCTCTACCTGGCCCTGCAGGTCTATGGAGTCGAGGTGCCT
  GCAGAGGGTGAGGCCGAGAAGCCTTGGTGGCAGGTATTTAGTGATGACCTTACCAAGCCAATCATTGACA
  ATATTGTGTCTGATCTCATTCAGATTTATACTATGGATACAGAGATCCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203196 representing NM_197989
  Red=Cloning site Green=Tags(s)
  
  MEAVRPDSCESGPAAPRAEERPAPEARVHFRVTRFIMEAGVKLGMQSIPIATACTIYHKFFCEINLDAYD
  LYLVAMSSIYLAGKVEEQHLRTRDIINVSHRYFNPGSEPLELDSRFWELRDSIVQCELLMLRVLRFQVSF
  QHPHKYLLHYLISLKNWLNRYSWQRTPISVTAWALLRDSYHGGLCLRFQAQHLAVAVLYLALQVYGVEVP
  AEGEAEKPWWQVFSDDLTKPIIDNIVSDLIQIYTMDTEIP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_197989
• ORF Size: 750 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_197989.1, NP_932106.1
• RefSeq Size: 1243 bp
• RefSeq ORF: 753 bp
• Locus ID: 69109
• UniProt ID: Q8QZR8
• Cytogenetics: 11 B5
• Gene Summary: Activating cyclin for the cyclin-associated kinase CDK10.[UniProtKB/Swiss-Prot Function]