Tmem109 (NM_134142) Mouse Tagged ORF Clone

CAT#: MG203020

Tmem109 (tGFP-tagged) - Mouse transmembrane protein 109 (Tmem109)

Product Images

Circular map for MG203020

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tmem109
• Synonyms: 1110006I15Rik; AA958958; C77652
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203020 representing NM_134142
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGCGCACACAGCACTCCGTTATGGAGCAGACATCTGCTCAAAGCTGTCCTGATGGTCCTCGTGG
  CCCTTTTCCTCGTCCACTCCGCATCAGCCCAGTCTCATCGAGAGTTTGCCTCACCTGGTCAACAGAAGAA
  GGAGACCTCAGCTGATATTTTGACCCAGATTGGCCGATCTCTGAAGGAGATGTTGGATACCTGGCTAGGG
  CCAGAGACCATGCATGTGATTTCAGAGACCCTGTTGCAGGTGATGTGGGCCATCTCATCAGCCATCTCTG
  TGGCCTGCTTTGCCCTGTCTGGGATTGCTGCCCAGTTGCTGAGTGCCCTGGGGCTGGATGGTGAACAACT
  CACCCAGGGCTTGAAGCTCAGCCCTAGCCAAGTCCAGACCCTTCTACTGTGGGGAGCAGCAGCACTGGTC
  ATCTACTGGTTGCTGTCCCTGCTCCTAGGCTTGGTCTTGGCCTTGCTAGGGAGGATCCTGGGGGGTCTGA
  AACTTGTTCTTTTTGTCGCTGGCTTTGTGGCCCTGGTGAGGTCTGTGCCCGACCCATCTACCCGGGCCTT
  GATGCTCTTGGCCTTGTTAACTCTCTTTGCCTTGCTGAGCCGGCTCACTGGCTCCAGGAGCTCAGGGAGC
  CACCTGGAAGCCAAGGTGAGAGGGCTGGAGCGCCAGATAGAAGAGCTTAGAGGGAGGCAGAGGCGAGCAG
  CCAAGATGCCCAGGAGCATGGAAGAAGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203020 representing NM_134142
  Red=Cloning site Green=Tags(s)
  
  MAGAHSTPLWSRHLLKAVLMVLVALFLVHSASAQSHREFASPGQQKKETSADILTQIGRSLKEMLDTWLG
  PETMHVISETLLQVMWAISSAISVACFALSGIAAQLLSALGLDGEQLTQGLKLSPSQVQTLLLWGAAALV
  IYWLLSLLLGLVLALLGRILGGLKLVLFVAGFVALVRSVPDPSTRALMLLALLTLFALLSRLTGSRSSGS
  HLEAKVRGLERQIEELRGRQRRAAKMPRSMEEE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134142
• ORF Size: 729 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134142.1, NP_598903.1
• RefSeq Size: 1943 bp
• RefSeq ORF: 732 bp
• Locus ID: 68539
• UniProt ID: Q3UBX0
• Cytogenetics: 19 A
• Gene Summary: May mediate cellular response to DNA damage by protecting against ultraviolet C-induced cell death (PubMed:20060811). Can form voltage-gated calcium and potassium channels in vitro (By similarity).[UniProtKB/Swiss-Prot Function]