Tpk1 (NM_013861) Mouse Tagged ORF Clone
CAT#: MG203016
- TrueORF®
Tpk1 (tGFP-tagged) - Mouse thiamine pyrophosphokinase (Tpk1)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_013861" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Tpk1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG203016 representing NM_013861
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCATGCCTTTACCCCGTTGGAACCCCTGCTACCTACGGGGAACTTGAAATACTGCCTTGTGGTTC TTAATCAGCCTTTGGATGCACGATTTCGCCATCTTTGGAAAAAAGCTCTCTTAAGAGCCTGTGCTGATGG GGGTGCCAACCACTTATATGATCTCACTGAAGGAGAGAGAGAAAGCTTCTTGCCTGAATTCGTCAGTGGG GACTTTGATTCTATTAGGCCTGAAGTCAAAGAGTACTACACCAAGAAGGGCTGTGATCTTATTTCAACTC CTGACCAAGACCACACTGACTTTACCAAGTGTCTTCAAGTGCTCCAAAGGAAGATAGAGGAAAAGGAACT GCAGGTTGACGTGATTGTGACACTGGGAGGTCTCGGTGGGCGTTTTGACCAAATCATGGCCTCTGTGAAT ACCCTTTTCCAAGCCACTCACATCACTCCTGTGCCGATTATAATAATCCAAAAGGACTCTCTCATCTACC TCCTCCAACCCGGGAAGCACAGGCTCCATGTAGACACTGGAATGGAAGGGAGCTGGTGTGGCCTGATTCC TGTTGGACAGCCTTGCAACCAGGTGACGACAACAGGCCTGAAATGGAACCTCACAAATGATGTTCTTGGC TTTGGAACACTGGTCAGTACTTCTAACACCTACGATGGGTCCGGCCTTGTCACTGTGGAAACTGACCACC CACTCCTCTGGACCATGGCCATCAAGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203016 representing NM_013861
Red=Cloning site Green=Tags(s) MEHAFTPLEPLLPTGNLKYCLVVLNQPLDARFRHLWKKALLRACADGGANHLYDLTEGERESFLPEFVSG DFDSIRPEVKEYYTKKGCDLISTPDQDHTDFTKCLQVLQRKIEEKELQVDVIVTLGGLGGRFDQIMASVN TLFQATHITPVPIIIIQKDSLIYLLQPGKHRLHVDTGMEGSWCGLIPVGQPCNQVTTTGLKWNLTNDVLG FGTLVSTSNTYDGSGLVTVETDHPLLWTMAIKS TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_013861 |
ORF Size | 729 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013861.4 |
RefSeq Size | 2563 bp |
RefSeq ORF | 732 bp |
Locus ID | 29807 |
UniProt ID | Q9R0M5 |
Cytogenetics | 6 B2.1 |
Gene Summary | Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201189 | Tpk1 (untagged) - Mouse thiamine pyrophosphokinase (Tpk1), (10ug) |
USD 300.00 |
|
MR203016 | Tpk1 (Myc-DDK-tagged) - Mouse thiamine pyrophosphokinase (Tpk1) |
USD 300.00 |
|
MR203016L3 | Lenti ORF clone of Tpk1 (Myc-DDK-tagged) - Mouse thiamine pyrophosphokinase (Tpk1) |
USD 600.00 |
|
MR203016L4 | Lenti ORF clone of Tpk1 (mGFP-tagged) - Mouse thiamine pyrophosphokinase (Tpk1) |
USD 600.00 |
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