Pithd1 (NM_025411) Mouse Tagged ORF Clone
CAT#: MG202570
- TrueORF®
Pithd1 (tGFP-tagged) - Mouse RIKEN cDNA 1110049F12 gene (1110049F12Rik)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_025411" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Pithd1 |
Synonyms | 1110049F12Rik; AA408179; Trp26 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG202570 representing NM_025411
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCACGGCCACAGCCACGGCGGGGGCGGCTGTCGCTGCGCCGCTGAGCGCGAGGAGCCGCCCGAGC AGCGCGGCCTGGCCTACGGGCTGTACCTGCGCATCGACCTGGAGCGACTGCAGTGCCTCAACGAGAGCCG CGAGGGCAGCGGCCGCGGCGTCTTCAAGCCGTGGGAGGAGCGGACCGACCGCTCCAAGTTTGTTGAAAGT GATGCGGACGAAGAGCTCCTGTTTAATATTCCGTTTACGGGCAATGTCAAGCTGAAAGGCGTCATCATAA TGGGCGAGGATGATGACTCGCACCCCTCGGAGATGAGACTGTACAAGAACATTCCACAGATGTCATTTGA TGACACAGAAAGGGAGCCAGAGCAGACCTTCAGTCTGAACCGAGACATTACAGGAGAATTAGAATATGCT ACGAAAATCTCCAGGTTTTCAAATGTCTATCATCTTTCCATTCATATTTCAAAAAACTTTGGAGCAGATA CGACGAAGATCTTTTATATTGGCCTGCGGGGAGAGTGGACTGAGCTTCGCCGGCATGAGGTGACCATCTG CAACTATGAAGCGTCAGCCAACCCAGCAGACCACCGGGTGCATCAGGTCACCCCGCAGACACACTTCATT TCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202570 representing NM_025411
Red=Cloning site Green=Tags(s) MSHGHSHGGGGCRCAAEREEPPEQRGLAYGLYLRIDLERLQCLNESREGSGRGVFKPWEERTDRSKFVES DADEELLFNIPFTGNVKLKGVIIMGEDDDSHPSEMRLYKNIPQMSFDDTEREPEQTFSLNRDITGELEYA TKISRFSNVYHLSIHISKNFGADTTKIFYIGLRGEWTELRRHEVTICNYEASANPADHRVHQVTPQTHFI S TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_025411 |
ORF Size | 666 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025411.4, NP_079687.3 |
RefSeq Size | 1533 bp |
RefSeq ORF | 636 bp |
Locus ID | 66193 |
UniProt ID | Q8BWR2 |
Cytogenetics | 4 D3 |
Gene Summary | Promotes megakaryocyte differentiation by up-regulating RUNX1 expression (PubMed:25134913). Regulates RUNX1 expression by activating the proximal promoter of the RUNX1 gene and by enhancing the translation activity of an internal ribosome entry site (IRES) element in the RUNX1 gene (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210296 | Pithd1 (untagged) - Mouse RIKEN cDNA 1110049F12 gene (1110049F12Rik), (10ug) |
USD 330.00 |
|
MR202570 | Pithd1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110049F12 gene (1110049F12Rik) |
USD 300.00 |
|
MR202570L3 | Lenti ORF clone of Pithd1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110049F12 gene (1110049F12Rik) |
USD 600.00 |
|
MR202570L4 | Lenti ORF clone of Pithd1 (mGFP-tagged) - Mouse RIKEN cDNA 1110049F12 gene (1110049F12Rik) |
USD 600.00 |
{0} Product Review(s)
Be the first one to submit a review