Mis12 (NM_025993) Mouse Tagged ORF Clone
CAT#: MG202174
- TrueORF®
Mis12 (tGFP-tagged) - Mouse MIS12 homolog (yeast) (Mis12)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_025993" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Mis12 |
Synonyms | 2510025F08Rik; AU044651; Misc12 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG202174 representing NM_025993
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGTGGATCCGATGGCCTATGAGGCCCAGTTCTTTGGCTTCACACCACAGACTTGCCTGCTGAGGA TCTACGTAGCATTTCAAGACCACCTGTTTGAAGTGATGCAGGCTGTTGAACAGGTTATCCTAAAGAAGCT GGAGGACATTCCAAACTGTGAGATCACCCCTGTCCAGACTCGGAAATGCACAGAGAAGTTTCTTTGCTTC ATGAAAGGACGCTTCGATAACCTTTTTGGCAAAATGGAGCAGCTGATTTTGCAGTCGATTTTGTGTATTC CTCCAAACATCCTGCTTCCTGAAGACAAGTGTCAGGAGACGAATCCTTTCAGTGAAGAGAAACTCGAGCT TCTCCAACAGGAAATCAAAGAGTTACAGGAGAAATACAAGGTTGAGTTGTGCACTGAGCAGGCCCTTCTT GCAGAATTAGAGGAGCAGAAAACTGTTAAGGCCAAACTCAGAGAGACCTTGACTTTCTTTGATGAGCTTG AAAACATCGGCAGATATCAAGGAACTAGTAACTTTAGGGAGAGTTTGGCATCCCTGGTCCAGAGCTGCAG AAAACTTCAGAGCATTAGAGACAATGTAGAAAAAGAAAGCAGGAGACTGGAAACACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202174 representing NM_025993
Red=Cloning site Green=Tags(s) MSVDPMAYEAQFFGFTPQTCLLRIYVAFQDHLFEVMQAVEQVILKKLEDIPNCEITPVQTRKCTEKFLCF MKGRFDNLFGKMEQLILQSILCIPPNILLPEDKCQETNPFSEEKLELLQQEIKELQEKYKVELCTEQALL AELEEQKTVKAKLRETLTFFDELENIGRYQGTSNFRESLASLVQSCRKLQSIRDNVEKESRRLETQ TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_025993 |
ORF Size | 618 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025993.3 |
RefSeq Size | 2766 bp |
RefSeq ORF | 621 bp |
Locus ID | 67139 |
UniProt ID | Q9CY25 |
Cytogenetics | 11 B4 |
Gene Summary | Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. Essential for proper kinetochore microtubule attachments.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210495 | Mis12 (untagged) - Mouse MIS12 homolog (yeast) (Mis12), (10ug) |
USD 330.00 |
|
MR202174 | Mis12 (Myc-DDK-tagged) - Mouse MIS12 homolog (yeast) (Mis12) |
USD 300.00 |
|
MR202174L3 | Lenti ORF clone of Mis12 (Myc-DDK-tagged) - Mouse MIS12 homolog (yeast) (Mis12) |
USD 600.00 |
|
MR202174L4 | Lenti ORF clone of Mis12 (mGFP-tagged) - Mouse MIS12 homolog (yeast) (Mis12) |
USD 600.00 |
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