Rhoq (NM_145491) Mouse Tagged ORF Clone

CAT#: MG202150

Rhoq (tGFP-tagged) - Mouse ras homolog gene family, member Q (Rhoq)

Product Images

Circular map for MG202150

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rhoq
• Synonyms: Arhq; Tc10; TC10A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202150 representing NM_145491
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCACGGGCCCGGCGCGCTGATGCTGAAGTGCGTGGTGGTCGGCGACGGGGCGGTGGGCAAGACGT
  GCCTGCTCATGAGCTATGCCAACGACGCCTTCCCCGAGGAGTACGTGCCCACTGTCTTCGACCACTACGC
  AGTCAGCGTCACCGTGGGGGGCAAGCAGTACCTCTTGGGACTCTATGACACAGCTGGACAGGAAGACTAT
  GATCGTCTGAGGCCTTTATCTTACCCCATGACTGACGTCTTCCTCATATGCTTCTCCGTGGTAAATCCAG
  CCTCGTTTCAAAATGTAAAGGAAGAGTGGGTACCAGAGCTAAAGGAATATGCGCCAAACGTCCCCTTCTT
  GTTAATAGGAACTCAGATTGATCTCCGAGATGACCCCAAAACTTTAGCAAGACTGAATGACATGAAAGAA
  AAACCTGTCTGTGTGGAACAAGGACAGAAACTAGCAAAAGAGATAGGTGCATGCTGCTATGTGGAGTGTT
  CAGCTTTAACCCAGAAGGGTTTGAAGACTGTGTTTGATGAGGCTATCATAGCCATTTTAACTCCAAAGAA
  ACACACAGTAAAAAAAAGAATAGGATCAAGATGTATAAACTGTTGTTTGATTACG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202150 representing NM_145491
  Red=Cloning site Green=Tags(s)
  
  MAHGPGALMLKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVSVTVGGKQYLLGLYDTAGQEDY
  DRLRPLSYPMTDVFLICFSVVNPASFQNVKEEWVPELKEYAPNVPFLLIGTQIDLRDDPKTLARLNDMKE
  KPVCVEQGQKLAKEIGACCYVECSALTQKGLKTVFDEAIIAILTPKKHTVKKRIGSRCINCCLIT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145491
• ORF Size: 615 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145491.2, NP_663466.2
• RefSeq Size: 4159 bp
• RefSeq ORF: 618 bp
• Locus ID: 104215
• UniProt ID: Q8R527
• Cytogenetics: 17 56.9 cM
• Gene Summary: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia (By similarity).[UniProtKB/Swiss-Prot Function]