Rbpms (BC030397) Mouse Tagged ORF Clone

CAT#: MG201952

Rbpms (tGFP-tagged) - Mouse RNA binding protein gene with multiple splicing (cDNA clone MGC:40656 IMAGE:4225078)

Product Images

Circular map for MG201952

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rbpms
• Synonyms: 2010300K22Rik; 2700019M19Rik; AU017537; hermes; RBP-MS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201952 representing BC030397
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGGCGGCGGCAAAGCCGAGAAGGAGAACACCCCGAGCGAGGCCAACCTTCAGGAGGAGGAGGTCC
  GGACCCTATTTGTCAGCGGTCTGCCTCTGGACATCAAGCCCCGAGAGCTGTACCTGCTCTTCAGACCATT
  CAAGGGCTATGAAGGTTCTCTCATAAAGCTCACATCTAAACAGCCCGTAGGCTTTGTCAGTTTTGACAGT
  CGCTCAGAAGCAGAGGCCGCAAAGAACGCTTTGAACGGCATCCGCTTCGATCCTGAAATCCCGCAAACGC
  TACGACTAGAGTTTGCTAAGGCAAACACGAAGATGGCCAAGAACAAACTCGTAGGGACTCCAAACCCCAG
  TACTCCTCTGCCCAACACTGTACCTCAGTTCATTGCCAGGGAGCCATATGAGCTCACAGTACCTGCACTT
  TACCCCAGTAGCCCTGAAGTTTGGGCCCCGTACCCTCTGTACCCAGCGGAGTTAGCGCCTGCTCTTCCTC
  CTCCTGCCGCTTTCACCTACCCCGCTTCACTGCATGCCCAGATGCGCTGGATCCCTCCCTCCGAGGCCAC
  TTCCCAGGGCTGGAAGTCCCGGCAGTTCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201952 representing BC030397
  Red=Cloning site Green=Tags(s)
  
  MNGGGKAEKENTPSEANLQEEEVRTLFVSGLPLDIKPRELYLLFRPFKGYEGSLIKLTSKQPVGFVSFDS
  RSEAEAAKNALNGIRFDPEIPQTLRLEFAKANTKMAKNKLVGTPNPSTPLPNTVPQFIAREPYELTVPAL
  YPSSPEVWAPYPLYPAELAPALPPPAAFTYPASLHAQMRWIPPSEATSQGWKSRQFC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC030397
• ORF Size: 593 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC030397, AAH30397
• RefSeq Size: 1124 bp
• RefSeq ORF: 593 bp
• Locus ID: 19663
• Cytogenetics: 8 A4
• Gene Summary: Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA.[UniProtKB/Swiss-Prot Function]