Cbx5 (NM_001076789) Mouse Tagged ORF Clone
CAT#: MG201823
- TrueORF®
Cbx5 (tGFP-tagged) - Mouse chromobox homolog 5 (Drosophila HP1a) (Cbx5), transcript variant 2
"NM_001076789" in other vectors (2)
Frequently bought together (4)
Other products for "Cbx5"
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Cbx5 |
Synonyms | 2610029O15Rik; C75991; HP1; Hp1a; Hp1alpha |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG201823 representing NM_001076789
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAAGAAGACCAAGAGGACAGCCGACAGCTCTTCTTCAGAGGATGAGGAGGAATATGTGGTGGAAA AGGTGTTGGACAGGCGCATGGTTAAGGGGCAAGTGGAATATCTGTTGAAGTGGAAAGGCTTTTCTGAGGA GCACAATACTTGGGAACCTGAGAAGAACTTGGATTGTCCTGAACTAATTTCTGAGTTTATGAAAAAGTAT AAGAAGATGAAGGAGGGTGAAAACAATAAGCCCAGGGAGAAATCAGAAGGAAACAAGAGGAAATCCAGTT TCTCCAACAGCGCTGATGATATTAAATCTAAAAAAAAGAGAGAGCAAAGCAATGATATCGCTCGGGGCTT TGAGAGAGGACTGGAACCAGAAAAGATCATCGGAGCAACAGATTCCTGCGGTGACTTAATGTTCTTAATG AAATGGAAAGACACAGATGAAGCTGACCTGGTTCTTGCAAAAGAAGCTAACGTGAAGTGTCCACAGATTG TGATAGCATTTTATGAAGAGAGACTGACGTGGCACGCATATCCAGAGGATGCGGAAAACAAAGAAAAAGA AAGCGCGAAGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201823 representing NM_001076789
Red=Cloning site Green=Tags(s) MGKKTKRTADSSSSEDEEEYVVEKVLDRRMVKGQVEYLLKWKGFSEEHNTWEPEKNLDCPELISEFMKKY KKMKEGENNKPREKSEGNKRKSSFSNSADDIKSKKKREQSNDIARGFERGLEPEKIIGATDSCGDLMFLM KWKDTDEADLVLAKEANVKCPQIVIAFYEERLTWHAYPEDAENKEKESAKS TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001076789 |
ORF Size | 576 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001076789.2 |
RefSeq Size | 8801 bp |
RefSeq ORF | 576 bp |
Locus ID | 12419 |
UniProt ID | Q61686 |
Cytogenetics | 15 F3 |
Gene Summary | Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins (By similarity).[UniProtKB/Swiss-Prot Function] |
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