Fam162a (NM_027342) Mouse Tagged ORF Clone

CAT#: MG201149

Fam162a (tGFP-tagged) - Mouse RIKEN cDNA 2310056P07 gene (2310056P07Rik)

Product Images

Circular map for MG201149

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fam162a
• Synonyms: 2310056P07Rik; HGTD-P
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201149 representing NM_027342
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAGCCTCGGCGGCCTGCGCCTGGCGGCTGGACATTGTCTTAGGTTATATGAAAGAAATGCTTCCT
  CGTCTCTAAGGTTTACCAGAAACACTGACTTGAAGAGGATCAATGGATTCTGCACCAAGCCACAGGAAAG
  TCCCAAAACTCCAACCCAGTCTTACAGACACGGGGTGCCGCTGCACAAGCCCACAGATTTTGAGAAGAAG
  ATCCTGCTGTGGTCAGGCCGCTTCAAGAAGGAGGAAGAGATCCCAGAGACAATCTCATTTGAGATGCTTG
  ATGCTGCGAAGAACAAGCTCCGGGTGAAGGTCAGCTATCTAATGATTGCCCTGACCGTGGCAGGATGCAT
  CTATATGGTTATTGAGGGCAAGAAGGCTGCGAAAAGACATGAGTCTTTAACAAGCTTGAACCTGGAAAGG
  AAAGCCCGTCTGAGAGAGGAGGCAGCTATGAAGGCCAAAACAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201149 representing NM_027342
  Red=Cloning site Green=Tags(s)
  
  MWSLGGLRLAAGHCLRLYERNASSSLRFTRNTDLKRINGFCTKPQESPKTPTQSYRHGVPLHKPTDFEKK
  ILLWSGRFKKEEEIPETISFEMLDAAKNKLRVKVSYLMIALTVAGCIYMVIEGKKAAKRHESLTSLNLER
  KARLREEAAMKAKTD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027342
• ORF Size: 465 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027342.2
• RefSeq Size: 635 bp
• RefSeq ORF: 468 bp
• Locus ID: 70186
• UniProt ID: Q9D6U8
• Cytogenetics: 16 B3
• Gene Summary: Proposed to be involved in regulation of apoptosis; the exact mechanism may differ between cell types/tissues. May be involved in hypoxia-induced cell death of transformed cells implicating cytochrome C release and caspase activation (such as CASP9) and inducing mitochondrial permeability transition. May be involved in hypoxia-induced cell death of neuronal cells probably by promoting release of AIFM1 from mitochondria to cytoplasm and its translocation to the nucleus; however, the involvement of caspases has been reported conflictingly.[UniProtKB/Swiss-Prot Function]