Edf1 (NM_021519) Mouse Tagged ORF Clone

CAT#: MG201022

Edf1 (tGFP-tagged) - Mouse endothelial differentiation-related factor 1 (Edf1)

Product Images

Circular map for MG201022

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Edf1
• Synonyms: 0610008L11Rik; AA409425
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201022 representing NM_021519
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGAGTGACTGGGACACCGTGACGGTGCTGCGCAAGAAGGGCCCCACGGCCGCACAGGCCAAGT
  CTAAGCAGGCCATCTTAGCGGCTCAGCGACGAGGAGAAGATGTGGAGACATCCAAGAAATGGGCTGCTGG
  CCAGAACAAGCAGCATTCCATTACTAAAAACACAGCCAAGCTCGACCGGGAGACCGAGGAGCTGCATCAC
  GACAGGGTGACCCTGGAGGTGGGCAAAGTGATCCAGCGGGGCCGGCAGAGCAAAGGCCTGACCCAGAAGG
  ACCTGGCCACGAAAATCAATGAGAAGCCGCAAGTCATCGCAGACTATGAAAGTGGACGGGCCATTCCGAA
  TAACCAGGTTTTGGGCAAAATTGAGAGAGCCATCGGCCTCAAGCTCCGGGGGAAGGACATCGGGAAGCCT
  ATCGAGAAGGGGCCGAAGGCGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201022 representing NM_021519
  Red=Cloning site Green=Tags(s)
  
  MAESDWDTVTVLRKKGPTAAQAKSKQAILAAQRRGEDVETSKKWAAGQNKQHSITKNTAKLDRETEELHH
  DRVTLEVGKVIQRGRQSKGLTQKDLATKINEKPQVIADYESGRAIPNNQVLGKIERAIGLKLRGKDIGKP
  IEKGPKAK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021519
• ORF Size: 444 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021519.2
• RefSeq Size: 678 bp
• RefSeq ORF: 447 bp
• Locus ID: 59022
• UniProt ID: Q9JMG1
• Cytogenetics: 2 A3
• Gene Summary: Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. Might function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism (By similarity).[UniProtKB/Swiss-Prot Function]