Lyz2 (NM_017372) Mouse Tagged ORF Clone

CAT#: MG201008

Lyz2 (tGFP-tagged) - Mouse lysozyme (Lyzs)

Product Images

Circular map for MG201008

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lyz2
• Synonyms: AI326280; Lys; Lysm; Lyzf2; Lyzs; Lzm; Lzm-s1; Lzp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201008 representing NM_017372
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACTCTCCTGACTCTGGGACTCCTCCTGCTTTCTGTCACTGCTCAGGCCAAGGTCTATGAACGTT
  GTGAGTTTGCCAGAACTCTGAAAAGGAATGGAATGGCTGGCTACTATGGAGTCAGCCTGGCCGACTGGGT
  GTGTTTAGCTCAGCACGAGAGCAATTATAACACACGAGCTACAAACTACAACCGTGGAGACCAAAGCACT
  GACTATGGGATATTTCAGATCAATAGCCGATACTGGTGTAATGATGGCAAAACCCCAAGAGCTGTGAATG
  CCTGTGGGATCAATTGCAGTGCTCTGCTGCAGGATGACATCACTGCAGCCATACAATGTGCAAAGAGGGT
  GGTGAGAGATCCCCAAGGCATTCGAGCATGGGTGGCATGGCGAGCACACTGTCAAAACCGAGATCTGTCC
  CAGTATATTCGGAACTGCGGAGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201008 representing NM_017372
  Red=Cloning site Green=Tags(s)
  
  MKTLLTLGLLLLSVTAQAKVYERCEFARTLKRNGMAGYYGVSLADWVCLAQHESNYNTRATNYNRGDQST
  DYGIFQINSRYWCNDGKTPRAVNACGINCSALLQDDITAAIQCAKRVVRDPQGIRAWVAWRAHCQNRDLS
  QYIRNCGV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017372
• ORF Size: 444 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017372.3
• RefSeq Size: 1271 bp
• RefSeq ORF: 447 bp
• Locus ID: 17105
• UniProt ID: P08905
• Cytogenetics: 10 65.34 cM
• Gene Summary: Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Lyz2 is active against a range of Gram-positive and Gram-negative bacteria. More effective than Lyz1 in killing Gram-negative bacteria. Lyz1 and Lyz2 are equally effective in killing Gram-positive bacteria.[UniProtKB/Swiss-Prot Function]