Cyb5a (NM_025797) Mouse Tagged ORF Clone

CAT#: MG200799

Cyb5 (tGFP-tagged) - Mouse cytochrome b-5 (Cyb5)

Product Images

Circular map for MG200799

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cyb5a
• Synonyms: 0610009N12Rik; Cyb5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200799 representing NM_025797
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGCAGTCAGACAAGGATGTGAAGTACTACACCCTGGAAGAGATTCAGAAGCACAAAGACAGCA
  AGAGCACCTGGGTGATCCTGCATCATAAGGTGTACGATCTGACCAAGTTTCTCGAAGAGCATCCTGGTGG
  AGAAGAAGTCCTAAGAGAGCAAGCTGGGGGTGATGCTACCGAGAATTTTGAGGACGTCGGGCACTCTACG
  GATGCACGAGAACTGTCCAAAACATACATCATCGGGGAACTCCATCCAGATGACAGATCAAAGATAGCCA
  AGCCTTCGGATACTCTTATCACTACTGTGGAGTCTAATTCCAGTTGGTGGACCAACTGGGTGATCCCAGC
  CATCTCAGCCCTGGCTGTAGCTCTGATGTATCGCCTCTACATGGCAGAAGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200799 representing NM_025797
  Red=Cloning site Green=Tags(s)
  
  MAGQSDKDVKYYTLEEIQKHKDSKSTWVILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHST
  DARELSKTYIIGELHPDDRSKIAKPSDTLITTVESNSSWWTNWVIPAISALAVALMYRLYMAED
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025797
• ORF Size: 402 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025797.4
• RefSeq Size: 750 bp
• RefSeq ORF: 405 bp
• Locus ID: 109672
• UniProt ID: P56395
• Cytogenetics: 18 57.53 cM
• Gene Summary: Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases. It is also involved in several steps of the sterol biosynthesis pathway, particularly in the C-5 double bond introduction during the C-5 desaturation.[UniProtKB/Swiss-Prot Function]