Mrap (NM_029844) Mouse Tagged ORF Clone

CAT#: MG200684

Mrap (tGFP-tagged) - Mouse melanocortin 2 receptor accessory protein (Mrap)

Product Images

Circular map for MG200684

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mrap
• Synonyms: 1110025G12Rik; C21ORF61; Falp; ORF61
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200684 representing NM_029844
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAACGGGACCGACGCCTCTGTCCCGCTCACCAGCTATGAGTATTACCTGGACTACATAGACCTCA
  TTCCTGTGGACGAGAAGAAGCTGAAAGCCAACAAGCATTCCATTGTCATCGCCCTGTGGTTGAGCCTGGC
  TACCTTCGTGGTGCTCCTCTTTCTCATCCTGCTCTACATGTCCTGGTCGGGCTCCCCACAGATGAGGCAC
  AGTCCCCAACCCCAGCCAATATGTTCATGGACTCACAGCTTCAACCTCCCTCTGTGCCTCCGGAGGGCCT
  CCCTGCAGACAACAGAGGAGCCAGGAAGGAGAGCTGGCACTGACCAGTGGTTAACGCAGCAGAGTCCTTC
  TGCCTCAGCCCCGGGGCCCCTGGCTCTCCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200684 representing NM_029844
  Red=Cloning site Green=Tags(s)
  
  MANGTDASVPLTSYEYYLDYIDLIPVDEKKLKANKHSIVIALWLSLATFVVLLFLILLYMSWSGSPQMRH
  SPQPQPICSWTHSFNLPLCLRRASLQTTEEPGRRAGTDQWLTQQSPSASAPGPLALP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029844
• ORF Size: 381 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029844.4
• RefSeq Size: 993 bp
• RefSeq ORF: 384 bp
• Locus ID: 77037
• UniProt ID: Q9D159
• Cytogenetics: 16 C3.3
• Gene Summary: Modulator of melanocortin receptors (MC1R, MC2R, MC3R, MC4R and MC5R). Acts by increasing ligand-sensitivity of melanocortin receptors and enhancing generation of cAMP by the receptors. Required both for MC2R trafficking to the cell surface of adrenal cells and for signaling in response to corticotropin (ACTH). May be involved in the intracellular trafficking pathways in adipocyte cells (By similarity).[UniProtKB/Swiss-Prot Function]