Bri3 (NM_018772) Mouse Tagged ORF Clone

CAT#: MG200643

Bri3 (tGFP-tagged) - Mouse brain protein I3 (Bri3)

Product Images

Circular map for MG200643

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bri3
• Synonyms: I3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200643 representing NM_018772
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCACAAGCCCCTGTTGCAGGAGCGCCCGCCCGCCTACAACCTGGAGGCCGGCCAGGGCGACTACG
  CGTGCGGCCCGCACGGCTACGGGGCCATCCCCACCGCGCCCCCGCCGCCGCCCTACCCCTACCTCGTCAC
  AGGGATCCCCACCAGCCACCCCCGGGTCTATAACATCCACAGTCGAACGGTCACCCGGTATCCTGCCAAC
  TCCATCGTCGTGGTCGGAGGCTGCCCGGTCTGCAGGGTTGGTGTCCTGGAGTACTGCTTCACCTGCCTGG
  GCATCTTCCTGGCCATCGTCCTGTTCCCTTTTGGGTTCCTCTGCTGCTTTGCACTGAGGAAGCGAAGATG
  CCCCAACTGTGGAGCGGTCTTTACT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200643 representing NM_018772
  Red=Cloning site Green=Tags(s)
  
  MDHKPLLQERPPAYNLEAGQGDYACGPHGYGAIPTAPPPPPYPYLVTGIPTSHPRVYNIHSRTVTRYPAN
  SIVVVGGCPVCRVGVLEYCFTCLGIFLAIVLFPFGFLCCFALRKRRCPNCGAVFT
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018772
• ORF Size: 375 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018772.4, NP_061242.3
• RefSeq Size: 954 bp
• RefSeq ORF: 378 bp
• Locus ID: 55950
• UniProt ID: P28662
• Cytogenetics: 5 G2
• Gene Summary: Participates in tumor necrosis factor-alpha (TNF)-induced cell death (Ref.4). May be a target of Wnt/beta-catenin signaling in the liver (By similarity).[UniProtKB/Swiss-Prot Function]