Polr3k (NM_025901) Mouse Tagged ORF Clone

CAT#: MG200406

Polr3k (tGFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide K (Polr3k)

Product Images

Circular map for MG200406

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Polr3k
• Synonyms: 12.3kDa; 1500004O14Rik; AI196849; AU014893; C11; RPC10; RPC11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG200406 representing NM_025901
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTTGTTCTGCCCGGGCTGCGGGAACGGACTCATTGTGGAGGAGGGGCAGCGTTGCCATCGCTTCG
  CCTGCAACACTTGCCCCTACGTCCATAATATCACCCGTAAGGTAACAAACCGAAAGTATCCAAAGCTAAA
  AGAAGTGGACGACGTGCTTGGTGGAGCAGCTGCATGGGAGAATGTGGACTCTACTGCAGAGCCATGTCCA
  AAATGCGAACATCCCCGTGCCTACTTCATGCAGCTTCAGACGCGTTCTGCAGACGAGCCAATGACCACCT
  TCTATAAGTGCTGCAATGCTCAGTGTGGACATCGCTGGAGGGAT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG200406 representing NM_025901
  Red=Cloning site Green=Tags(s)
  
  MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAEPCP
  KCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025901
• ORF Size: 324 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025901.3, NP_080177.1
• RefSeq Size: 2925 bp
• RefSeq ORF: 327 bp
• Locus ID: 67005
• UniProt ID: Q9CQZ7
• Cytogenetics: 2 H4
• Gene Summary: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).[UniProtKB/Swiss-Prot Function]