Tnp1 (NM_009407) Mouse Tagged ORF Clone
CAT#: MG200020
- TrueORF®
Tnp1 (tGFP-tagged) - Mouse transition protein 1 (Tnp1)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_009407" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Tnp1 |
Synonyms | Stp-1; Tp-1; TP1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG200020 representing NM_009407
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGACCAGCCGCAAGCTAAAGACTCATGGCATGAGGAGAGGCAAGAACCGAGCTCCTCACAAGGGCG TCAAGAGAGGTGGAAGCAAGAGAAAATACCGGAAGAGCGTCCTGAAAAGTAGGAAACGGGGCGATGATGC AAGTCGCAATTACCGATCCCACTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200020 representing NM_009407
Red=Cloning site Green=Tags(s) MSTSRKLKTHGMRRGKNRAPHKGVKRGGSKRKYRKSVLKSRKRGDDASRNYRSHL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_009407 |
ORF Size | 165 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009407.2, NP_033433.1 |
RefSeq Size | 431 bp |
RefSeq ORF | 168 bp |
Locus ID | 21958 |
UniProt ID | P10856 |
Cytogenetics | 1 36.94 cM |
Gene Summary | Plays a key role in the replacement of histones to protamine in the elongating spermatids of mammals (PubMed:10781074, PubMed:15163613, PubMed:15189834, PubMed:15083521, PubMed:28366643). In condensing spermatids, loaded onto the nucleosomes, where it promotes the recruitment and processing of protamines, which are responsible for histone eviction (PubMed:28366643). The histone H2AFB1-HIST1H2BA/TH2B dimer is required for loading of TNP1 onto chromatin (PubMed:28366643).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204986 | Tnp1 (untagged) - Mouse transition protein 1 (Tnp1), (10ug) |
USD 150.00 |
|
MR200020 | Tnp1 (Myc-DDK-tagged) - Mouse transition protein 1 (Tnp1) |
USD 150.00 |
|
MR200020L3 | Lenti ORF clone of Tnp1 (Myc-DDK-tagged) - Mouse transition protein 1 (Tnp1) |
USD 450.00 |
|
MR200020L4 | Lenti ORF clone of Tnp1 (mGFP-tagged) - Mouse transition protein 1 (Tnp1) |
USD 450.00 |
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