Myo7a (NM_001256082) Mouse Untagged Clone

CAT#: MC229715

Myo7a (untagged) - Mouse myosin VIIA (Myo7a), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myo7a
• Synonyms: Hdb; Myo7; nmf371; polka; sh-1; sh1; USH1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229715 representing NM_001256082
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTGAAGTCAGGCCAGGAGTTTGATGTGCCCATCGGGGCCGTGGTGAAGCTCTGCGACTCGGGCC
  AGATCCAGGTGGTGGATGATGAAGACAATGAACACTGGATATCCCCTCAGAATGCCACGCACATCAAGCC
  AATGCACCCCACATCGGTGCACGGCGTGGAGGACATGATCCGCCTGGGGGATCTCAACGAGGCAGGCATC
  CTTCGAAACCTTCTCATTCGCTACCGGGACCACCTCATCTATACCAGCTGTGGAGGACGGACGTACACAG
  GTTCCATCCTGGTGGCCGTGAACCCCTACCAGCTGCTCTCCATCTACTCGCCAGAGCACATCCGCCAGTA
  CACCAACAAGAAGATAGGGGAGATGCCCCCCCACATCTTCGCCATTGCTGACAACTGCTACTTCAACATG
  AAACGCAACAACCGGGACCAGTGCTGTATTATCAGCGGGGAGTCGGGAGCTGGCAAGACAGAGAGCACAA
  AGTTGATCCTGCAGTTCCTGGCAGCCATCAGTGGACAGCACTCATGGATCGAGCAGCAGGTGCTGGAGGC
  CACCCCGATCCTGGAAGCATTTGGGAACGCCAAGACCATCCGCAACGACAACTCTAGCCGCTTTGGCAAG
  TACATTGACATCCACTTTAACAAGCGTGGTGCCATCGAGGGCGCCAAAATAGAGCAATACCTGCTGGAGA
  AGTCACGTGTCTGCCGCCAGGCCCCTGACGAGAGGAACTATCACGTGTTCTACTGTATGCTGGAGGGCAT
  GAATGAGGAGGAGAAGAAGAAACTGGGCCTAGGCCAGGCCGCTGACTACAACTACTTGGCCATGGGTAAC
  TGCATCACCTGTGAGGGCCGCGTGGACAGTCAGGAGTATGCCAACATCCGCTCTGCCATGAAGGTTCTCA
  TGTTCACAGACACGGAGAACTGGGAGATCTCGAAGCTTCTGGCTGCCATCCTACACATGGGCAATCTGCA
  GTATGAGGCCCGGACATTTGAGAACTTGGATGCGTGTGAAGTCCTCTTCTCCCCATCGCTGGCCACGGCA
  GCTTCTCTGCTCGAGGTGAACCCCCCAGACCTGATGAGCTGCCTCACCAGCCGCACCCTCATCACCCGTG
  GGGAGACGGTGTCCACCCCTCTCAGCAGGGAACAGGCGCTGGATGTGCGAGATGCCTTTGTCAAGGGCAT
  CTATGGGCGGCTCTTTGTGTGGATTGTGGAGAAGATCAACGCAGCAATCTACAAGCCACCCCCCCTGGAA
  GTGAAGAACTCTCGCCGGTCCATCGGTCTCCTGGACATCTTTGGATTTGAGAACTTCACTGTGAACAGCT
  TCGAGCAGCTCTGCATTAACTTTGCCAATGAGCACCTGCAGCAATTCTTCGTGCGGCACGTGTTCAAGCT
  GGAGCAGGAGGAGTACGACCTGGAGAGCATCGACTGGTTGCACATTGAGTTCACTGACAACCAGGAAGCA
  CTGGACATGATTGCCAACCGGCCTATGAACGTCATCTCCCTCATCGATGAGGAGAGCAAGTTCCCCAAGG
  GCACGGATGCCACCATGCTGCATAAGCTGAACTCACAGCACAAGCTCAATGCCAACTACGTGCCACCCAA
  GAACAGCCACGAGACCCAGTTTGGAATCAACCACTTTGCGGGTGTTGTCTATTATGAGAGTCAAGGCTTC
  CTGGAGAAGAACCGAGACACCCTGCATGGGGACATCATCCAGCTGGTCCACTCTTCCCGGAACAAGTTCA
  TAAAGCAGATTTTCCAAGCTGACGTTGCCATGGGTGCCGAGACCAGGAAGCGCTCGCCTACACTCAGCAG
  CCAGTTCAAGCGGTCTCTGGAGCTGCTGATGCGCACACTGGGCGCCTGCCAGCCCTTCTTTGTGCGTTGT
  ATCAAACCCAATGAGTTCAAGAAGCCCATGCTCTTCGACCGGCACTTGTGTGTACGCCAGCTGCGATATT
  CGGGCATGATGGAGACAATCCGCATCCGCCACGCAGGCTACCCCATTCGCTACAGCTTTGTGGAGTTTGT
  GGAGCGCTACCGGGTACTGCTGCCTGGTGTGAAGCCAGCATACAAGCAGGGTGACCTCCGAGGGACATGC
  CAGCGCATGGCTGAGGCTGTGCTGGGCACGCACGATGACTGGCAGATTGGCAAAACCAAGATCTTTCTGA
  AGGACCACCATGACATGTTGCTGGAGGTGGAGCGGGACAAGGCCATCACAGACAGAGTCATTCTCCTCCA
  GAAGGTTATCCGGGGCTTCAAAGACAGGTCCAACTTCCTGAGACTGAAGAGTGCTGCCACACTGATCCAG
  AGGCACTGGCGGGGCCACCACTGTAGGAAAAACTATGAGCTGATTCGTCTTGGCTTCCTGCGGCTGCAGG
  CCCTGCACCGCTCCCGGAAGCTGCACAAGCAGTACCGCCTGGCCAGACAGCGCATAATAGAGTTCCAGGC
  CCGCTGCCGGGCCTATCTGGTGCGCAAGGCCTTCCGCCACCGCCTCTGGGCCGTGATCACCGTGCAGGCC
  TATGCCCGAGGCATGATTGCCCGCCGGCTACACCGCCGCCTCCGGGTTGAGTACCAGCGGCGCCTCGAGG
  CAGAGAGGATGCGTCTGGCAGAGGAGGAGAAACTCCGAAAGGAGATGAGTGCCAAGAAGGCCAAAGAGGA
  GGCTGAGCGCAAGCATCAGGAGCGCCTGGCTCAGCTAGCCCGCGAGGATGCGGAGCGGGAACTGAAGGAG
  AAGGAGGAGGCTCGGAGGAAGAAGGAACTGCTGGAGCAGATGGAGAAGGCCCGCCACGAACCCATCAACC
  ACTCAGATATGGTGGACAAGATGTTTGGCTTCCTGGGGACTTCAGGCAGCCTGCCAGGCCAGGAAGGCCA
  GGCGCCTAGTGGCTTTGAGGACCTAGAGCGCGGACGGAGGGAGATGGTGGAAGAGGATGTTGACGCTGCC
  CTGCCCCTGCCTGATGAAGACGAGGAGGACCTTTCTGAGTACAAATTCGCCAAGTTTGCTGCCACCTACT
  TCCAGGGCACAACCACACACTCCTACACCCGGAGGCCTCTCAAGCAGCCGCTGCTCTACCACGACGATGA
  GGGTGACCAGCTGGCGGCGCTGGCTGTCTGGATCACCATCCTCCGGTTCATGGGGGACCTCCCAGAGCCC
  AAGTACCACACAGCCATGAGCGACGGCAGTGAGAAGATCCCAGTGATGACTAAGATCTACGAGACCCTAG
  GCAAGAAGACATATAAGAGGGAGCTGCAGGCCTTGCAGGGCGAGGGCGAGACCCAGCTCCCTGAGGGGCA
  GAAGAAGACCAGTGTGAGACACAAGTTGGTACACTTGACACTGAAGAAAAAGTCCAAACTCACAGAAGAG
  GTGACCAAGAGGCTGAACGATGGGGAATCCACGGTACAGGGCAACAGCATGCTGGAGGATCGGCCCACCT
  CAAATCTAGAGAAGCTGCACTTCATCATCGGCAACGGCATCCTGCGGCCTGCGCTGCGGGACGAGATTTA
  CTGCCAGATCAGTAAGCAGCTCACACACAACCCATCCAAGAGCAGCTATGCCAGGGGCTGGATCCTCGTG
  TCGCTCTGTGTGGGCTGCTTCGCCCCCTCTGAGAAGTTCGTTAAGTACCTGCGGAACTTCATCCACGGAG
  GCCCACCTGGCTATGCTCCTTACTGTGAGGAGCGCCTGAGGAGGACCTTTGTCAACGGAACTCGGACACA
  GCCACCCAGCTGGCTGGAGCTGCAGGCCACCAAGTCCAAGAAGCCCATCATGTTGCCCGTGACCTTCATG
  GATGGGACCACCAAGACCCTGCTAACAGATTCAGCAACTACAGCCAGGGAGCTGTGCAATGCTCTGGCTG
  ACAAGATCTCACTCAAGGACCGCTTTGGCTTCTCCCTCTACATCGCTCTGTTCGATAAGGTGTCCTCCCT
  GGGCAGCGGCAGTGACCATGTCATGGATGCCATCTCTCAGTGTGAGCAGTACGCCAAGGAGCAGGGTGCT
  CAGGAGCGCAACGCCCCATGGAGGCTCTTCTTTAGAAAGGAGGTCTTCACACCCTGGCACAACCCCTCGG
  AGGACAACGTGGCCACGAACCTCATCTACCAGCAGGTGGTGCGAGGAGTCAAGTTTGGGGAGTACAGGTG
  TGAGAAGGAGGACGACCTGGCTGAGCTGGCTTCTCAGCAGTACTTTGTGGACTATGGTTCTGAGATGATT
  CTGGAGCGCCTGCTGAGCCTCGTGCCCACTTACATCCCTGACCGTGAGATCACACCGCTGAAGAATCTTG
  AGAAGTGGGCACAGCTGGCCATTGCTGCCCACAAGAAGGGAATTTATGCCCAGAGGAGAACTGACTCCCA
  GAAGGTCAAAGAGGATGTGGTCAATTATGCCCGTTTCAAGTGGCCCTTGCTCTTCTCCAGGTTTTACGAA
  GCTTACAAATTCTCAGGCCCTCCCCTCCCCAAGAGCGACGTCATCGTGGCTGTCAACTGGACGGGTGTGT
  ACTTCGTGGACGAGCAGGAGCAGGTGCTTCTGGAGCTGTCCTTCCCGGAGATCATGGCTGTGTCCAGCAG
  TAGGGGAACAAAGATGATGGCCCCCAGCTTTACCCTGGCCACCATCAAAGGAGATGAGTACACCTTCACA
  TCCAGCAATGCTGAGGACATCCGTGACCTGGTGGTCACCTTTCTGGAGGGGCTACGGAAGAGGTCTAAGT
  ATGTGGTGGCACTGCAGGACAATCCTAACCCTGCTGGTGAGGAGTCAGGCTTCCTCAGCTTCGCCAAGGG
  AGACCTCATCATCCTTGACCATGATACTGGTGAGCAGGTCATGAACTCAGGCTGGGCCAACGGCATCAAC
  GAGAGGACCAAGCAGCGCGGCGACTTCCCCACTGACTGTGTATACGTCATGCCCACTGTCACCTTGCCAC
  CAAGGGAGATTGTGGCCCTGGTCACTATGACCCCAGACCAGAGGCAGGATGTCGTCCGGCTCCTGCAGCT
  TCGCACAGCAGAGCCAGAGGTGCGCGCCAAGCCCTACACGCTAGAGGAGTTCTCCTACGACTACTTCAGG
  CCCCCACCCAAGCACACGCTGAGCCGTGTCATGGTGTCCAAGGCCCGCGGTAAGGACAGGCTGTGGAGCC
  ACACACGAGAGCCCCTCAAGCAGGCCCTGCTCAAGAAGATCCTGGGCAGTGAAGAACTCTCCCAGGAAGC
  CTGCATGGCCTTTGTAGCTGTGCTCAAGTACATGGGCGACTACCCATCCAAGAGGATGCGATCCGTCAAT
  GAGCTCACTGACCAGATCTTTGAGTGGGCACTCAAGGCTGAGCCCCTCAAGGATGAGGCCTACGTGCAGA
  TCCTGAAGCAGCTGACTGACAATCACATCAGGTACAGCGAAGAGAGGGGCTGGGAACTGCTGTGGCTGTG
  CACGGGCCTCTTCCCGCCCAGCAACATCCTCCTGCCTCATGTTCAGCGGTTTCTGCAGTCCCGCAAGCAC
  TGTCCTCTTGCCATTGACTGCCTGCAGAGGCTCCAGAAAGCCCTGAGAAATGGCTCCCGGAAGTACCCTC
  CGCACCTGGTGGAGGTGGAGGCCATCCAACATAAGACTACCCAGATCTTCCACAAGGTCTACTTCCCCGA
  TGACACGGACGAGGCTTTTGAGGTGGAGTCCAGCACCAAGGCCAAGGACTTCTGCCAGAACATCGCCAGC
  CGGCTGCTGCTCAAGTCTTCCGAGGGATTCAGCCTTTTTGTCAAAATCGCAGATAAGGTCATCAGCGTCC
  CAGAGAATGATTTCTTCTTTGACTTTGTCCGACACCTGACAGACTGGATAAAGAAAGCACGGCCCATCAA
  GGACGGAATCGTGCCCTCACTAACCTACCAGGTGTTCTTCATGAAGAAGCTGTGGACCACCACAGTGCCG
  GGCAAGGACCCCATGGCTGACTCCATCTTCCACTATTACCAGGAACTGCCCAAATATCTCCGAGGCTACC
  ACAAGTGCACCCGGGAGGAGGTGCTGCAGCTGGGCGCACTCATCTACAGGGTCAAGTTTGAGGAGGACAA
  ATCCTACTTCCCTAGCATCCCCAAGTTGCTGAGGGAGCTGGTACCCCAGGACCTAATCCGGCAGGTCTCA
  CCTGATGACTGGAAACGGTCTATTGTCGCCTACTTCAACAAACATGCGGGGAAGTCCAAGGAGGAAGCCA
  AGCTGGCCTTCCTCAAACTCATCTTCAAGTGGCCCACCTTTGGCTCAGCCTTCTTTGAGGTGAAGCAAAC
  TACAGAACCAAACTTCCCAGAGATTCTCTTAATTGCCATCAACAAGTACGGGGTCAGCCTCATCGATCCC
  AGAACCAAGGACATCCTGACTACTCACCCCTTCACCAAGATCTCCAACTGGAGTAGTGGCAACACCTACT
  TCCACATCACCATTGGGAACTTGGTCCGTGGGAGCAAACTGCTCTGTGAGACATCGCTGGGATACAAAAT
  GGATGATCTTCTGACTTCCTACATCAGCCAGATGCTCACAGCCATGAGCAAGCAGAGGAACTCCAGGAGT
  GGAAGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256082
• Insert Size: 6519 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256082.1, NP_001243011.1
• RefSeq Size: 7214 bp
• RefSeq ORF: 6519 bp
• Locus ID: 17921
• Cytogenetics: 7 53.57 cM
• Gene Summary: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. In the retina, plays an important role in the renewal of the outer photoreceptor disks. Plays an important role in the distribution and migration of retinal pigment epithelial (RPE) melanosomes and phagosomes, and in the regulation of opsin transport in retinal photoreceptors. Mediates intracellular transport of RPE65 in the retina pigment epithelium. In the inner ear, plays an important role in differentiation, morphogenesis and organization of cochlear hair cell bundles. Motor protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.