Zswim8 (NM_001252081) Mouse Untagged Clone

CAT#: MC229671

Zswim8 (untagged) - Mouse zinc finger SWIM-type containing 8 (Zswim8), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zswim8
• Synonyms: 2310021P13Rik; 4832404P21Rik; Kiaa0913; mKIAA0913
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229671 representing NM_001252081
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGATGTTCGCGGAGTGGGAGGACGGCGAGCGCTTCTCGTTTGAGGATTCCGACCGCTTTGAGG
  AGGATTCACTCTGTTCGTTCATTTCCGAGGCCGAGAGCCTTTGCCAGAACTGGCGAGGATGGCGCAAACA
  GTCAGCGGGGCCCAATTCCCCCACTGGAGGCGGTGGCGGAGGTGGCAGTGGCGGTACCAGAACGCGAGAT
  GGATTGGTAATCCCATTGGTGGAGCTGTCAGCAAAGCAGGTGGCATTTCACATCCCATTTGAAGTGGTAG
  AGAAAGTTTATCCTCCAGTGCCAGAACAACTCCAACTCCGAATTGCTTTTTGGAGCTTCCCTGAGAATGA
  AGAGGACATTCGTCTGTATTCATGCCTAGCCAATGGCAGTGCGGATGAGTTTCAGCGAGGGGATCAGCTG
  TTCCGAATGAGGGCTGTGAAAGACCCGCTGCAGATAGGGTTCCATCTGAGCGCCACAGTGGTACCACCGC
  AAATGGTCCCACCCAAGGGGGCCTACAATGTAGCTGTGATGTTTGACCGCTGCCGGGTCACTTCTTGTAG
  CTGTACCTGTGGGGCCGGGGCCAAATGGTGCACCCATGTCGTGGCACTCTGCCTCTTCCGCATTCACAAC
  GCATCTGCAGTCTGCCTGCGGGCTCCAGTCTCAGAGTCCCTGTCTCGGCTACAAAGGGACCAGCTTCAAA
  AATTTGCTCAGTACCTTATCAGTGAGCTTCCTCAGCAGATTCTCCCCACAGCCCAGCGTCTTCTAGACGA
  GCTCCTTTCCTCCCAGTCCACAGCCATCAACACAGTGTGTGGGGCTCCGGACCCTACAGCAGGGCCCTCA
  GCTTCAGACCAGAGCACTTGGTATTTGGATGAGTCAACACTCACTGACAACATAAAGAAGACACTACATA
  AGTTCTGTGGCCCCTCCCCTGTGGTCTTCAGTGATGTAAACTCTATGTATCTCTCTTCCACGGAACCTCC
  TGCTGCTGCTGAGTGGGCATGTCTGCTGCGGCCTCTGAGGGGTCGAGAGCCTGAGGGTGTCTGGAACCTG
  CTTAGCATTGTTCGAGAGATGTTCAAGCGAAGGGACAGCAATGCTGCCCCCTTGCTGGAAATACTCACTG
  ACCAGTGCCTCACCTATGAACAGATAACAGGCTGGTGGTATAGTGTGCGCACCTCAGCTTCACACAGCAG
  TGCCAGTGGTCACACAGGCCGTAGCAATGGGCAGTCAGAGGTAGCAGCCCATGCATGTGCAAGTATGTGC
  GACGAGATGGTTACCCTCTGGAGGCTGGCTGTGCTGGACCCTGCCCTCAGCCCTCAGCGCCGCCGGGAAC
  TGTGTGCACAGCTGCGTCAGTGGCAACTGAAGGTGATTGAGAATGTCAAGCGGGGCCAGCACAAGAAGAC
  CCTAGAGAGGCTCTTCCCTGGCTTCCGGCCAGCTGTGGAGGCCTGCTACTTTAACTGGGAAGAGGCTTAT
  CCTCTTCCTGGTGTTACCTACAGTGGCACTGACCGGAAGCTAGCCCTGTGCTGGGCCCGGGCCCTGCCTG
  CCAGGCCAGGAGCCTCTAGATCTGGGGGCCTGGAAGAGTCCCGGCCCCGACCTCTTCCTACTGAGCCAGC
  TGTGAGGCCCAAGGAACCTGGGGCCAAACGCAAAGGATTGGGTGAGGGGATCTCCTCACAGCGGGGCCCC
  CGCCGCCTCTCTGCCGAAGGAGGAGATAAGGCTCTGCATAAGATGGGTCCAAGTGGGGGCAAAGCCAAGG
  TACTGGGTGGGACTGGCAGCGGGGGCAAGAGCTCAGCAGGCAGTGGGAGCAAACGGCGGCTAAGCAGTGA
  AGACAGCTCCCTGGAACCAGACCTGGCAGAGATGAGCCTGGATGACAGCAGCCTGGCCCTGGGTGCAGAG
  GCCAGCACCTTTGGTGGATTCCCTGAGAGCCCTCCACCCTGTCCTTCCTCGGTTGGCTCCAGAGGACCTT
  CCACCTTCCTTCCTGAGCCCCCAGATACTTATGAGGAAGATGCTGGTGTGTACTTTTCAGAAGGACCTGA
  GCCTCCCACAGCCTCTGCAGACCACCCTGGTCTGCTGCCTGGGGAGGTCTGTACCCGAGATGACCTCCCT
  TCCACAGACGACAGTGGCAGTGGGCTACACAAAACCAAAGAAGCAGCTCCTGCAGTTGGAGAGGAGGATG
  ACGACTACCAAGCATATTACCTGAATGCCCAGGATGGGGCTGGAGGCGAGGAGGAGAAGGCGGAGGGCGG
  GACTGGGGAGGAGCATGACCTGTTTGCTGGTTTGAAGCCACTGGAACAGGAGAGCCGAATGGAGGTGTTG
  TTTGCCTGCGCTGAGGCCCTGCATGCCCATGGCTACAGCAATGAGGCCTCCCGCCTCACTGTGGAGCTTG
  CCCAGGACCTGCTAGCCAACCCACCTGACCTCAAGGGCAAGAAAAACAAGGTGTCCACAAGCCGTCAGAC
  CTGGGTGGCTACCAACACTCTGACCAAAGCAGCCTTCCTGTTGACAGTGCTTAGTGAGCGCCCTGAGCAC
  CACAGCCTGGCCTTCCGAGTTGGCATGTTTGCGCTGGAGCTACAGCGGCCTCCTGCTTCAACCAAGGCCT
  TGGAGGTAAAATTGGCATATCAGGAATCTGAGGTAGCTGCTCTGCTCAAGAAGATCCCTCGGGGCCCCAG
  TGAAATGAGTACCATTCGATGCCGGGCAGAAGAACTTCGGGAGGGTACACTGTGTGACTATCGGCCTGTC
  TTGCCCCTCATGTTGGCCAGTTTCATCTTTGATGTTCTCTGTGCTCCAGTGGTTTCTCTCACGGGTTCCC
  GGCCCCCAAGTCGAAACTGGACTAACGAGATGCCTGGAGATGAGGAGCTAGGATTTGAAGCAGCAGTTGC
  TGCCTTGGGCATGAAGACAACAGTGAGTGAGGCAGAACATCCCCTCCTATGTGAAGGCACACGTCGGGAG
  AAGGGTGACCTGGCCTTAGCACTCATGATCACCTACAAGGATGACCAGGCCAAGCTCAAGAAGATCCTAG
  ACAAACTCTTGGACCGAGAAAGCCAGACACATAAGCCACAGACCCTGAGTTCTTTCTACTCATCCAGCCG
  GCCGGCCACAGCCAACCAGAGATCTCCTTCAAAGCACGGGGCCCCATCTGCTCCCGGGGCCCTGCAGCCA
  CTGACTTCAAGCTCTGCAGGGCCTGCTCAGCCAGGGAATGTGGCAGGGGCTGGGCCAGGTCCCACTGAGG
  GCTTCACAGAGAAGAATGTACCCGAAAGTTCCCCACATTCCCCCTGTGAAGGTCTCCCACCTGAGGCAGC
  TTTGACTCCACGGCCGGAGGGGAAGGTTCCTAGCCGCCTAGCACTTGGCAGTCGTGGAGGTTATAATGGT
  CGAGGTTGGGGCTCTCCAGGGCGGCCCAAAAAGAAACACACAGGCATGGCCAGCATTGACAGCAGTGCCC
  CTGAAACCACATCGGACAGCTCTCCTACCTTAAGCCGACGGCCACTTCGAGGGGGCTGGGCCCCTACTTC
  TTGGGGTCGAGGACAAGACAGTGACAGCATTAGCAGCTCTTCCTCAGACTCTCTGGGCTCTTCATCCTCC
  AGTGGAAGCCGCCGGGCTAGTGCCAGTGGAGGGGCCCGGGCAAAGACAGTTGACGTTGGCAGGTGTTACA
  AAGGCCGCCGCCCTGAGAGTCATGCCCCCCACGTACCCAATCAGCCGTCAGAGGCAGCTGCACACTTCTA
  CTTCGAATTGGCGAAGACAGTTCTGATCAAGGCAGGGGGCAACAGCAGCACCTCCATTTTCACACATCCA
  TCTTCCTCAGGAGGCCACCAGGGTCCTCACCGCAACCTGCACCTTTGCGCCTTTGAGATTGGGCTTTATG
  CCCTAGGCCTGCATAACTTTGTTTCTCCTAACTGGCTCTCTCGTACCTATTCTTCCCATGTATCCTGGAT
  TACAGGGCAGGCCATGGAGATTGGCAGTGCAGCCTTGACTATACTGGTAGAATGCTGGGATGGGCACCTG
  ACACCCCCTGAGGTTGCATCGCTGGCCGACAGAGCGTCACGGGCACGAGACTCCAACATGGTGAGGGCAG
  CGGCAGAGCTGGCTCTAAGCTGCCTGCCTCATGCCCACGCACTGAACCCCAATGAGATTCAGCGGGCCTT
  GGTGCAGTGCAAGGAACAGGATAACCTGATGTTGGAGAAGGCCTGCATGGCCGTGGAAGAGGCAGCCAAG
  GGTGGGGGCGTATACCCTGAAGTGCTGTTTGAGGTTGCTCATCAGTGGTTCTGGCTTTATGAGGAGACAG
  CAGGCGGCTCGTCCACAGCTCGTGAAGGGGCTACAAGCTGTAGTGGCAGTGGGATGAGGGCCGCTGGGGA
  GGCTGGGCGGGGACTCCCTGAGGGTAGGGGTGCCCCAGGGACTGAACCTGTTACAGTGGCTGCGGCAGCA
  GTGACAGCAGCAGCCACAGTGGTTCCGGTCATCTCAGTGGGGTCCAGTTTATATCCAGGTCCAGGACTGG
  GGCATGGCCACTCCCCTGGCCTGCACCCCTACACTGCTCTCCAGCCCCACTTGCCCTGCAGCCCTCAGTA
  CCTCACCCACCCAGCTCACCCTGCCCACCCTATGCCTCATATGCCCCGGCCTGCCGTCTTCCCTGTGCCC
  AGCTCTGCATACCCACAGGGTGTGCATCCTGCATTCCTGGGGGCGCAATACCCTTACTCAGTGACTCCTC
  CCTCGCTTGCTGCCACAGCTGTATCTTTCCCTGTCCCTTCCATGGCTCCCATCACAGTCCATCCTTACCA
  CACAGAACCAGGGCTCCCACTGCCCACCAGTGTGGCCTTGAGCAGTGTCCATCCAGCATCTACGTTTCCA
  GCCATCCAGGGTGCCTCACTGCCTGCTCTGACCACACAGCCCAGCCCTCTGGTAAGCGGGGGTTTTCCAC
  CACCCGAAGAGGAGACACACAGTCAACCGGTCAATCCACATAGCCTGCACCATCTGCATGCTGCTTACCG
  TGTTGGGATGCTGGCACTGGAGATGCTAGGTCGCCGGGCACACAACGATCACCCCAACAACTTTTCCCGC
  TCCCCCCCTTACACTGATGATGTCAAATGGTTGCTGGGGCTGGCAGCAAAGCTGGGAGTGAACTACGTGC
  ACCAGTTCTGTGTGGGGGCAGCCAAGGGGGTGCTGAGCCCGTTTGTGCTGCAGGAGATCGTCATGGAGAC
  GCTGCAGCGGCTGAACCCCATTCATGCCCACAACCACCTTCGAGCCCCGGCCTTCCACCAACTGGTGCAG
  CGCTGTCAGCAGGCATACATGCAGTACATCCATCACCGCTTAATTCACCTGACTCCTGCCGACTACGACG
  ACTTTGTGAATGCAATCCGCAGCGCTCGCAGCGCCTTCTGCCTGACACCCATGGGCATGATGCAGTTCAA
  CGACATCCTACAGAACCTCAAACGCAGCAAACAGACCAAGGAGTTGTGGCAGCGGGTCTCTCTGGAGATA
  ACCACCTTCTCCCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252081
• Insert Size: 5478 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252081.1, NP_001239010.1
• RefSeq Size: 6073 bp
• RefSeq ORF: 5478 bp
• Locus ID: 268721
• UniProt ID: Q3UHH1
• Cytogenetics: 14 A3
• Gene Summary: Substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex that promotes target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs) (PubMed:33184237). The SCF-like E3 ubiquitin-protein ligase complex acts by catalyzing ubiquitination and subsequent degradation of AGO proteins (AGO1, AGO2, AGO3 and/or AGO4), thereby exposing miRNAs for degradation (By similarity). Specifically recognizes and binds AGO proteins when they are engaged with a TDMD target (By similarity). May also acts as a regulator of axon guidance: specifically recognizes misfolded ROBO3 and promotes its ubiquitination and subsequent degradation (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.