Camsap1 (NM_001276359) Mouse Untagged Clone

CAT#: MC229634

Camsap1 (untagged) - Mouse calmodulin regulated spectrin-associated protein 1 (Camsap1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Camsap1
• Synonyms: 9530003A05Rik; C77823; PRO2405
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229634 representing NM_001276359
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGACGCGGGCGGTCGCTGTGCCGCCGAAGGCTGGAGGAGGATGGAGGCGCCCCCGGAGGGCGCCG
  ACCTGGTGCCGCTGGACCGCTACGACGCGGCGCGGGCCAAGATCGCCGCCAACCTGCAGTGGATCTGTGC
  GAAGGCCTATGGCCTAGACAACATCCCTGAGGACCTCCGAGACCCGTTTTACATCGACCAGTATGAGCAG
  GAGCACATTAAGCCACCCGTTATCAAGCTTCTCCTGTCCAGTGAGCTGTATTGCCGTGTCTGCAGCCTCA
  TCCTAAAAGGGGACCAGGTGGCTACCTTGCAAGGACACCAGTCTGTCATCCAGGCCCTGTCCCGGAAGGG
  CATCTATGTGATGGAGAGTGATGATACCCCTGTGACAGATGCTGACCTCAGCCAGGCACCTATTAAGATG
  AGTGGCCACATGGCGATGGTGGATGCCCTGATGATGGCCTACACTGTGGAGATGATCAGCATTGAGAAGG
  TGGTGGCCAGTGTCAAGCGCTTTTCTACATTCAGCGCCTCAAAAGAACTTCCTTATGACCTCGAGGATGC
  CATGGTATTCTGGATCAATAAGGTAAATCTTAAAATGAGAGAGATAACAGAGAAAGAAGTTAAATTAAAA
  CAGCAGCCACTGGAAAGTCCCGCTCATCAAAAGGTCCGTTATCGGCGAGAGCACCTCTCTGCTAGGCAGT
  CACCCTACTTCCCGTTGTTGGAGGACTTGATGAGAGACGGCAGTGATGGCGCTGCTCTCTTAGCAGTGGT
  TCACTATTATTGCCCAGAGCAGATGAAACTGGATGATATCTGTCTGAAGGAGGTGCCATCAATGGCTGAC
  AGCCTCTATAACATCCGCCTTCTGAGGGAGTTTTCCAACGAGCATCTGAACAAGTGTTTCTATCTCACGC
  TGGAGGATATGCTGTATGCCCCCCTAGTGCTGAAGCCGAACGTTATGGTTTTTATCGCTGAGCTCTTCTG
  GTGGTTTGAGAACGTCAAACCAGATTTTGTTCAGCCCCGAGATATTCAAGAGCTGAAGGATGCTAAAACA
  GTGTTACAGCAGAAGAGCAGCCGGCCTCCTGTCCCTATCTCCAATGCAACCAAACGCAGCTTCCTGGGTA
  GCCCTGCTGCCATGAGCCCCGCTGACCAGCCACCATCCACCCAGCCTCTTGCTGAAGGCAGTCATCGGTA
  CCACCTCCACTCGGAAGAACCTGAGTGTCTTGGGAAAGGAGCTTCCACATTTAGTCCGTCCCATCCTTTG
  TTGCCACTGAGACAGAAACAGCAAAAAGTGTCACAGACAGAGGAAATCCCTGATCAGCGGCACAGGTCGA
  ATTCTTTAACTCGGGTTGATGGTCAGCCACGAGGTGCAATAGGAGCATGGCCAGATAAAAAAAACAGGCC
  TGTGTCACAGCCAACATCATTTGCTCTCCATCATGCTGCAAGTTGTGATGTGGATCCCAGTTCTGGTGAC
  AGTGTCAGCTTAGCTCGCTCTATCAGCAAAGATAGCTTGGCATCTAACATTATCCACCTGACCCCACAGA
  ACCAGCCCCATCCCTCCGCTGGAAAGAGCAATGGGAAAAGCCTCCTGAGCAATGTCAACATTGAGGATGA
  GGACGAGGAGCTTGTAGCCATCATCAGGACAGATGTGTCTCCTCCTTCCCCACAGATGCCCAGGACCTCA
  CCTCAGGCCCCAGGCCTGGTAGCAAGTATCAGGTCTCCCCAGAGACAGGCAGACACTTTGGAGAGTAAGC
  CTGACAGTTTTTACTTAGAACCCCTGATGCCAGCAGTACTCCGGCCAGCCAAAGAGAAGCAGATTACCAC
  TAAGGAAGATGAGCGTGGAGAAGGAAGGCCAAGGACCATCATGGCCAAGAGGCCTAGTGAGGGCTCCCAG
  CCTATGGTACGAAAGAAAGTAAGTGGTGGCCATGGCAGTCGTGACCTGAACAGAACTTTCACCCCAATTC
  CTTGTTCAGAATTTGCTGCGAGCATTGATCTTGCGGAGGTAGGACCACAGTCAGCAGAAGCTACAGGGGA
  AGGACAGCCTCTGGCCCTGGGTAGATTTGATACATTACCTCAGGGGCAGGCTGCTGATGGTTTCTTTCTC
  CATGTAGGCAGGGCTGAGGAAGATGAGGGGAGGTGGTACGTTGGTTCACAAAGTCCTAGCTCCCACGACT
  CAGAACCCTGGACCATTTTGAGGCAAGACTCGGACTCTGATGTAGTAGACGTAGAGGATACAGAGCAGGA
  TTTTATTGGTGAGGACCACCCTGTGGTTATCCCCAGATATGCTGGTGAGGAAGAGTCAGCCAAACTACAG
  GAGGATATGAAAGTGAAGGAACATGAAGACAAGGATGATGCCAGCGGCCGCTCAAGCCCGTGTCTGAGCA
  CCACTTCTCAGCTCAGCAGCATGTCCATGGCCAGTGGAAGTGTAAAGATGACCAGCTTTGCTGAAAGGAA
  GCTCCAGCGGCTCAACAGCTGTGAGACCAAGTCCAGCACCAGCAGCTCCCAGAAGACCACCCCTGATGCA
  TCTGAAAGCTGCCCAGCCCCTCTGACAACGTGGCGCCAGAAGAGGGAGCAGAGTCCAGGCAGGCACAGCA
  AGGACCCTGCCAGCCTGCTGGCTTCTGAGCTGGTGCAGCTGCACATGCAGCTGGAGGAAAAGCGCAGAGC
  TATTGAAGCCCAGAAGAAAAAGATGGAGGCGCTGTCCGCACGGCAGCGTCTGAAGCTGGGCAAGGCAGCC
  TTTCTGCATGTGGTAAAGAAAGGGAAGGCTGATGGTGCCCCACAGCCACTGAGACCCGAACACTTCACAA
  AGGAATTCACACAGCACAATGGGGAGGACTTGGATGATGGCACTTGTAAAACTGAAGGGTTCCTTGTCAA
  AGAAGAGCAGAGAGATCTCAGTGACGCTCAGGATGTGGCATTTGTCCAGCTACATAAGCCCAGGGACCCA
  GCTGCTCTGCATGATGGAGAGAAGCACAGAATGATTTCTACGGCCCTCCTCGAGGATAGTGTTGGTGAGG
  TAGATGTGAACGAGTGTGACCTGTCCATTGAGAAGCTCAATGAGACCATCAGTACATTGCAGCAGGCTAT
  ACTGAAGATCTCCCAGCAGCAAGAGCAGCTTCTCATGAAGTCCCCCACAGTGCCAACACCGGGCACTAAA
  AATAACTGCCAGGACCAAAAAATAAAGGCCCCAGTCCACTTTGTTGAGCCACTCTCTCCAACTGGCGTGC
  CTGGCCACCGCAAACCACCACGGCTTGGCCAGGGCCGGAATTCCCGCTCAGGAAGGCCGGCTGAACTGAA
  GGTTCCAAAAGACAGGCAGCAGGGTTGTTCTCGGAGCAAAACCCCAACACCCAGTGTGGAGACACTCCCA
  CAGTCAAGGTCTTTACCTCCAAGTACCCATCCACGGTCACCCTCCGACCCAGGCGGGGAACTCCCTGAGA
  AGTGTCTCTTCGACAGTTATAGGCTCCATGATGAGAGTAACCATCGGACATTTGTTCTGTCCTCTTGCAA
  AGATGCAAACATTGTATCAGAACAGGTGAACTTTAAAGAGGGTCTAGATACAAGTGTGAAAGAGGCAGGG
  CTGAGCTCCTCTACCATCACAGGCAAAGAACATACTCCAGTGGAAGAGCCGCTGAGGAGCAAGGCCAGCC
  TCATTGAGGTAGACCTCTCTGACCTGAAGGCCCCTGATGAGGATGGAGAGGTGGTTGGCCATGAGAGCTC
  GGTGGAGCTTGGTGGAGATAGTGACCAGAAGCCTGGGGTTGGTTTCTTCTTCAAGGATGAACAGAAAGCA
  GAAGATGAGCTTGCTAAGAAGCGGGCAGCCTTCCTTTTGAAACAGCAGCGCAAGGCTGAAGAGGCTCGTG
  CGCGCAAGCAGCAGCTGGAGGCAGAAGTGGAGCTCAAGCGAGATGAAGCCCGGCGTAAGGCTGAAGAGGA
  TCGACTACGGAAGGAGGAGGAGAAGGCAAGGCGAGAGCTCATTAAACAGGAATACTTACGGAGGAAGCAG
  CAGCAGGCCTTGGAGGAGCAAGGACTTGGCAAACCTAAATCAAAGCCTAAAAAGCCTCGGCCAAAGTCAG
  TTCACCGGGAAGAGTCTTACAGCGACTCTGGCACCAAGTGCTCTTCTACCCATAACTTGAGCCAAACTCA
  CTCTGGCTCCAGCCTATCCTTGGCATCTGCAGCAACAACAGAACCTGAGAGTGTTTATTCGGGGGGCACA
  CCTTCTCACCGAGTTGAATCACTGGAAGCTTTACCTATCCTGAGCCGCAACCCCAGTCGGAGCACAGACC
  GAGACTGGGAGACTGCATCAGCAGCTTCCTCTTTGGCCTCTGTGGCTGAGTACACAGGTCCTAAACTCTT
  TAAGGAGCCCAGTAGCAAATCAAACAAACCAATTATTCACAATGCCATCTCCCACTGCTGTCTGGCTGGA
  AAAGTGAATGAGCCTCACAAGAATTCAATATTGGAGGAGCTGGAGAAGTGTGATGCCAACCACTATATTA
  TTCTGTTCCGAGATGCGGGCTGCCAGTTCAGGGCACTTTACTGCTACCAACCCGACACTGAAGAAATCTA
  CAAACTCACTGGCACGGGGCCAAAAAGCATCACCAAGAAGATGATTGACAAACTATATAAATACAGCTCA
  GACCGAAAGCAGTTTAACCTGATCCCAGCCAAGACCATGTCAGTCAGCGTGGATGCACTCACGATCCATA
  ACCACTTGTGGCAGCCCAAGCGGCCCACGGTGCCAAAGAAGACCCAGACTCGTAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276359
• Insert Size: 4749 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276359.1, NP_001263288.1
• RefSeq Size: 7996 bp
• RefSeq ORF: 4749 bp
• Locus ID: 227634
• UniProt ID: A2AHC3
• Cytogenetics: 2 A3
• Gene Summary: Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization. Specifically recognizes growing microtubule minus-ends and stabilizes microtubules. Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization. In contrast to CAMSAP2 and CAMSAP3, tracks along the growing tips of minus-end microtubules without significantly affecting the polymerization rate: binds at the very tip of the microtubules minus-end and acts as a minus-end tracking protein (-TIP) that dissociates from microtubules after allowing tubulin incorporation. Through interaction with spectrin may regulate neurite outgrowth.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the shortest transcript and encodes the shortest protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data from different strains to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.