Ralgapb (NM_001291138) Mouse Untagged Clone

CAT#: MC229603

Ralgapb (untagged) - Mouse Ral GTPase activating protein, beta subunit (non-catalytic) (Ralgapb), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ralgapb
• Synonyms: AI507211; B230339M05Rik; p170
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229603 representing NM_001291138
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACTCTGAGTGGCGGTCACTGCATTTGGTGATTCAGAGTGATCAAGGCCATACCAGTGTGCTACACA
  GCTATCCAGAGAGTGTTGGGCGAGAGGTGGCGAATGCAGTGGTCCGTCCTCTTGGGCAGGCATTAGGTAC
  TTCTTCAGTGGCTGGTAGTGAGAGTTTATTAAAAACTGACAAAGAAGTAAAATGGACCATGGAAGTAATT
  TGCTATGGACTGACCCTTCCATTAGATGGAGAGACTGTAAAATATTGTGTTGATGTATATACAGATTGGA
  TTATGGCTTTGGTGTTGCCAAAAGATTCTATTCCATTACCCATTATTAAAGAACCTAATCTATATGTTCA
  AAGTATATTAAAACATTTACAAAATCTTTTTGTACCAAGACAGGAACAGGGCTCCAGTCAAATCCGACTA
  TGCTTACAAGTTCTGAGAGCCATTCAGAAACTGGCCCGGGAGTCATCCATCATGGCTCGAGAAACCTGGG
  AAGTCTTACTGTTGTTTCTTCTGCAAATTAATGACATACTTCTGGCCCCACCAACTGTTCAAGGTGGCAT
  TGCTGAGAATCTAGCAGAGAAGCTGATTGGTGTTCTCTTTGAAGTTTGGTTACTAGCTTGCACCCGGTGC
  TTCCCAACACCTCCTTATTGGAAGACAGCCAAGGAGATGGTGGCTAACTGGAGGCACCACCCTGCAGTGG
  TGGAGCAGTGGAGCAAGGTTATCTGTGCACTGACCTCCAGATTGCTCCGCTTTACATACGGTCCTTCATT
  TCCTCCATTTAAAGTTCCTGATGAAGATGCCAATCTCATCCCTCCAGAAATGGACAATGAGTGTATTGCA
  CAAACATGGTTTCGATTTTTACACATGCTAAGTAACCCCGTGGATCTGAGTAACCCAGCTGTTATAAGCT
  CAACTCCCAAGTTTCAAGAACAGTTCCTGAATGTGAGCGGGATGCCACAGGAGCTGAGCCAGTACCCCTG
  CCTTAAACATCTGCCTCAGATATTCTTTCGTGCCATGCGTGGAATCAGCTGTCTGGTGGATGCATTCTTA
  GGCATTTCTCGACCTCGATCAGACAGTGCTCCCCCAACACCCGTGAATAGATTAAGTATGCCCCAGAGTG
  CTGCTGTGAATACCACACCACCACATAACCGGAGGCACCGGGCTGTTACTGTGAACAAGGCCACAATGAA
  GACGAGTACAGTTACTACTGCTCACACCTCAAAAGTCCAGCACCAGGCATCCTCCACCTCTCCTCTGTCC
  AGTCCAAATCAGACTAGTTCAGAACCCAGGCCGCTGCCTGCCCCCCGAAGACCAAAGGTTAACAGCATCT
  TGAATCTCTTTGGATCGTGGTTATTCGATGCAGCATTTGTTCACTGTAAACTCCATAATGGAATAAACAG
  AGACAACAGCATGACTGCCATTGCAACCCAAGCTAGCATGGAGTTTCGACGGAAAGGGTCCCAAATGTCA
  ACAGACACCATGGTTTCCAACCCTGTGTTTGATGCCAGTGAATTTCCTGATAACTATGAAGCAGGAAGAG
  CTGAGGCTTGTGGGACATTATGTAGGATTTTTTGTAGCAAGAAGACTGGAGAAGAGATTCTGCCAGCTTA
  TTTATCCAGATTTTACATGCTTTTAATTCAAGGTTTGCAGATAAATGATTATGTATGCCATCCTGTCTTG
  GCCAGCGTTATTCTAAACTCCCCTCCTTTGTTCTGCTGTGACTTGAAAGGGATTGATGTTGTGGTTCCTT
  ACTTTATTTCAGCTCTTGAAACCATTTTGCCTGACAGAGAACTCTCAAAATTCAAAAGCTATGTAAATCC
  AACAGAATTGAGAAGGTCATCCATTAATATCCTGCTTTCTTTGTTGCCCCTCCCTCATCATTTTGGCACA
  GTCAGATCTGAGGTGGTCCTAGAAGGAAAGTTTAGTAATGATGACAGCTCTTCTTATGATAAACCAATAA
  CTTTTCTGTCCCTGAAGCTGAGACTTGTGAATATACTAATAGGTGCCTTGCAAACAGAAACGGACCCTAA
  CAACACCCAAATGATACTAGGGGCAATGTTAAATATTGTTCAAGATTCTGCACTTTTAGAAGCCATTGGT
  TGCCAAATGGAAATGGGTGGTGGAGAAAACAACCTGAAGAGTCATAGTCGCACAAACAGTGGCATTAGCT
  CAGCAAGTGGAGGAAGCACAGAGCCTACAACCCCTGATAGCGAGAGACCTGCTCAAGCTCTTCTGAGGGA
  TTATGCTCTTAATACAGATTCAGCTGCTGGGCTCCTGATTCGCAGCATTCATCTCGTCACTCAACGACTC
  AACTCCCAGTGGCGGCAAGATATGAGCATATCCTTGGCAGCTCTAGAGCTCCTCTCTGGCCTGGCAAAGG
  TGAAGGTGATGGTTGACTCAGGAGACCGGAAGCGTGCCATCAGTTCTGTGTGCAGCTACATCGTATACCA
  ATGCAGTCGGCCAGCTCCTCTTCACTCCCGGGATCTGCACTCCATGATAGTGGCAGCATTTCAGTGTCTC
  TGTGTCTGGCTGACAGAGCACCCTGATATGCTGGATGAAAAGGATTGCCTTAAGGAAGTACTGGAGATTG
  TAGAACTGGGTATCTCAGGAAGCAAATCCAAGAACAGTGAGCAAGAAGTCAAGTACAAAGGTGATAAGGA
  GCCGAACCCTGCATCCATGAGGGTAAAGGACGCCGCCGAAGCCACTCTAACATGTATCATGCAGTTGCTT
  GGCGCATTTCCTTCACCCAGTGGTCCTGCTTCTCCTTGTAGTCTGGTGAATGAGACCACTCTGATCAAAT
  ACTCCAGGCTGCCAACCATAAACAAGCATAGTTTCCGGTACTTTGTTTTGGATAACAGTGTCATCCTGGC
  AATGCTGGAGCAACCGCTTGGAAATGAGCAGAATGACTTTTTCCCCTCTGTCACTGTGCTGGTCCGGGGA
  ATGTCTGGAAGACTTGCGTGGGCTCAGCAGCTTTGCCTTTTACCCAGAGGAGCAAAAGCAAATCAGAAGC
  TTTTTGTGCCTGAACCTCGCCCAGTTCCTAAAAATGATGTTGGATTTAAATATTCTGTGAAACATCGGCC
  ATTTCCTGAAGAGGTGGACAAGATTCCTTTTGTAAAAGCAGACTTGAGCATTCCAGATTTACATGAAATT
  GTCACTGAAGAATTAGAAGAGAGGCATGAAAAATTAAGAAGTGGTATGGCACAACAAATTGCTTATGAGA
  TGCACCTGGAACAGCAGAGCGAGGGGGAACTGCAGAAGCGAAGCTTCCCTGACCCTGTTACGGACTGTAA
  GCCTCCACCTCCTGCCCAGGAATTTCAGACAGCCCGCCTTTTCCTTTCTCACTTTGGATTTTTATCCTTA
  GAGGCATTAAAGGAACCTGCAAATAGTCGTCTACCTCCTCATCTTATTGCACTCGATTCTACCATACCTG
  GATTTTTTGATGACATTGGGTATCTGGATCTCCTGCCATGCCGGCCTTTTGACACTGTTTTTATTTTCTA
  CATGAAGCCAGGTCAGAAAACAAACCAAGAGATTTTAAAGAATGTGGAGTCTTCCAGAAATGTTCAGCCA
  CATTTCCTAGAATTTTTGCTCTCCCTGGGCTGGTCAGTAGATGTGGGGAAACACCCCGGTTGGACAGGAC
  ATGTGTCCACCAGTTGGTCAATCAATAGTTGTGATGATGGTGAAGGGTCTGAACCAGATGAAATAACTTC
  CTCTGAAGACGTTGGGGCTAGCATTTTCAACGGGCAGAAGAAGGTGCTGTATTATGCTGATGCCCTAACA
  GAAATAGCTTTTGTGGTTCCTTCTCCTGTGGAGTCACTGACTGATTCATTGGAAAGTAACATCTCAGACC
  AAGATAGTGACTCAAATATGGATCTTATGCCGGGAATTCTGAAGCAGCCACCCCTGACACTTGAGTTGGT
  CCCCAATCACACAGACAGTCTTAATTCCTCACAGAGGCTCAGTCCCAGTTCCAGAATGAAGAAGCTGCCT
  CAGGGTCGCCCTGTGCCCCCGCTTGGACCTGAGACAAGAGTATCTGTAGTCTGGGTGGAACGCTACGATG
  ATATAGAAAACTTTCCACTCTCAGATCTGATGACAGAGATCAGCACTGGTGTAGAGACTACTGCAAATAG
  TAGCACTTCTCTGAGATCTACAACTCTTGAAAAAGAAGTCCCAGTAATCTTCATTCACCCTTTAAACACT
  GGATTGTTCCGGATAAAAATCCAAGGAGCCACTGGAAAATTTAACATGGTCATACCTCTGGTTGATGGGA
  TGATAGTCAGCCGTCGAGCACTTGGCTTTCTGGTGAGGCAGACGGTAATAAACATTTGTAGAAGAAAGAG
  ACTGGAGAGTGATTCCTACAGTCCACCACATGTCCGTCGGAAACAGAAAATCACTGACATTGTCAACAAA
  TATCGGAATAAGCAGTTGGAGCCAGAGTTTTATACTGCCCTTTTCCAGGAGGTTGGACTCAAGAACTGCA
  GTTCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291138
• Insert Size: 4488 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291138.1, NP_001278067.1
• RefSeq Size: 8390 bp
• RefSeq ORF: 4488 bp
• Locus ID: 228850
• Cytogenetics: 2 H1